Basonuclins 1 and 2, whose genes share a common origin, are proteins with widely different properties and functions

Basonuclin (bn) 1 possesses three separated pairs of zinc fingers and a nuclear localization signal. It is largely confined to the basal cells of stratified squamous epithelia and to reproductive germ cells. bn1 can shuttle between the nucleus and the cytoplasm, and its location is correlated with the proliferative potential of the cell. The recently discovered bn2 also possesses three separated pairs of zinc fingers and a nuclear localization signal. Conservation of the zinc fingers and the nuclear localization signal by bn1 and bn2 indicates a common origin. However, in contrast to bn1, bn2 is found in virtually every cell type and is confined to the nucleus. Bn2 but not bn1 colocalizes with SC35 in nuclear speckles and, therefore, is likely to have a function in nuclear processing of mRNA.

[1]  Jane Loveland,et al.  Genomic anatomy of the Tyrp1 (brown) deletion complex. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[2]  D. Cazalla,et al.  A Novel SR-Related Protein Is Required for the Second Step of Pre-mRNA Splicing , 2005, Molecular and Cellular Biology.

[3]  N. Kuldell,et al.  Zinc finger proteins : from atomic contact to cellular function , 2005 .

[4]  A. F. Neuwald,et al.  Proteomic analysis of interchromatin granule clusters. , 2004, Molecular biology of the cell.

[5]  S. Sinha,et al.  Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes. , 2004, Genomics.

[6]  P. Djian,et al.  Serial cultivation of chicken keratinocytes, a composite cell type that accumulates lipids and synthesizes a novel beta-keratin. , 2004, Differentiation; research in biological diversity.

[7]  P. Djian,et al.  Basonuclin 2: an extremely conserved homolog of the zinc finger protein basonuclin. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[8]  David L. Spector,et al.  Nuclear speckles: a model for nuclear organelles , 2003, Nature Reviews Molecular Cell Biology.

[9]  Manqiu Cao,et al.  High-throughput generation and engineering of recombinant human antibodies. , 2001, Journal of immunological methods.

[10]  G. Kopf,et al.  Function of basonuclin in increasing transcription of the ribosomal RNA genes during mouse oogenesis. , 2001, Development.

[11]  E. Hurt,et al.  The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans , 2000, Nature.

[12]  J. Yong,et al.  Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm. , 2000, Molecular cell.

[13]  S. Iuchi,et al.  Alternative subcellular locations of keratinocyte basonuclin , 2000, Experimental dermatology.

[14]  R. Lavker,et al.  Basonuclin in murine corneal and lens epithelia correlates with cellular maturation and proliferative ability. , 1999, Differentiation; research in biological diversity.

[15]  H. Green,et al.  Basonuclin, a zinc finger protein of keratinocytes and reproductive germ cells, binds to the rRNA gene promoter. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[16]  A. F. Neuwald,et al.  Purification and biochemical characterization of interchromatin granule clusters , 1999, The EMBO journal.

[17]  W. Tang,et al.  Translocation of the zinc finger protein basonuclin from the mouse germ cell nucleus to the midpiece of the spermatozoon during spermiogenesis. , 1998, Biology of reproduction.

[18]  H. Green,et al.  Nuclear localization of basonuclin in human keratinocytes and the role of phosphorylation. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[19]  E. Fuchs,et al.  An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids , 1997, The Journal of cell biology.

[20]  E. Wahle,et al.  Immunodetection of poly(A) binding protein II in the cell nucleus. , 1994, Experimental cell research.

[21]  H. Green,et al.  Association of basonuclin with ability of keratinocytes to multiply and with absence of terminal differentiation , 1994, The Journal of cell biology.

[22]  A. Krainer,et al.  Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors , 1994, The Journal of cell biology.

[23]  H. Green,et al.  Basonuclin: a keratinocyte protein with multiple paired zinc fingers. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[24]  T. Maniatis,et al.  Isolation of a complementary DNA that encodes the mammalian splicing factor SC35. , 1992, Science.

[25]  T. Maniatis,et al.  Associations between distinct pre‐mRNA splicing components and the cell nucleus. , 1991, The EMBO journal.

[26]  T. Maniatis,et al.  Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus , 1990, Nature.

[27]  D. Spector,et al.  Immunoelectron microscopic localization of snRNPs , 1983, Biology of the cell.