MOSQUITO HOSTS OF ARBOVIRUSES FROM INDIAN RIVER COUNTY, FLORIDA, DURING 1998

Adult mosquitoes were collected for virus isolation from two sites in Indian River County, FL, from May 5 through August 13, 1998 using dry ice-baited CDC light traps (81 trap-nights) and CDC gravid traps (254 trap-nights). A total of 46,150 female mosquitoes (923 mosquito-pools, 50 females/pool) were processed for virus isolation. These females represented 18 species of mosquitoes, with Culex nigripalpus comprising 77.4% of all mosquitoes collected, followed by Aedes infirmatus (4.9%), Ae. vexans (4.0%) and Cx. erraticus (2.4%). No St. Louis encephalitis (SLE) and eastern equine encephalitis (EEE) virus isolates were obtained. Keystone (KEY) and Tensaw (TEN) viruses were isolated from Ae. albopictus (one isolate of KEY); Anopheles crucians (two isolates of TEN); Cx. nigripalpus (one isolate of TEN and 2 isolates of KEY); Coquilletidia perturbans (two isolates of TEN); and Wyeomyia vanduzeei (one isolate of TEN). All isolates were obtained from mosquitoes collected in CDC light traps, except for the KEY virus isolate from Ae. albopictus, which was collected in a CDC gravid trap. The isolation of TEN virus from Wy. vanduzeei is a first record for Florida.

[1]  L. Stark,et al.  Frequency of Saint Louis Encephalitis Virus in Humans from Florida, USA: 1990–1999 , 2000, Journal of medical entomology.

[2]  N. Karabatsos,et al.  Arboviruses associated with mosquitoes from nine Florida counties during 1993. , 1996, Journal of the American Mosquito Control Association.

[3]  G. Kuno,et al.  Detecting bunyaviruses of the Bunyamwera and California serogroups by a PCR technique , 1996, Journal of clinical microbiology.

[4]  G. Chang,et al.  An integrated target sequence and signal amplification assay, reverse transcriptase-PCR-enzyme-linked immunosorbent assay, to detect and characterize flaviviruses , 1994, Journal of clinical microbiology.

[5]  T. Monath,et al.  St. Louis encephalitis: lessons from the last decade. , 1987, The American journal of tropical medicine and hygiene.

[6]  N. Karabatsos,et al.  Distribution of Bunyamwera serogroup viruses in North America, 1956-1984. , 1986, The American journal of tropical medicine and hygiene.

[7]  F. M. Wellings,et al.  Agents encountered during arboviral ecological studies: Tampa Bay area, Florida, 1963 to 1970. , 1972, The American journal of tropical medicine and hygiene.

[8]  W. D. Sudia,et al.  VECTOR STUDIES IN THE ST. LOUIS ENCEPHALITIS EPIDEMIC, TAMPA BAY AREA, FLORIDA, 1962. , 1964, The American journal of tropical medicine and hygiene.

[9]  K. E. Meadows,et al.  ISOLATION OF ST. LOUIS ENCEPHALITIS VIRUSES FROM MOSQUITOES IN THE TAMPA BAY AREA OF FLORIDA DURING THE EPIDEMIC OF 1962. , 1964, The American journal of tropical medicine and hygiene.

[10]  P. Reiter.  A portable battery-powered trap for collecting gravid Culex mosquitoes. , 1983 .

[11]  J. V. Lange,et al.  Indirect immunofluorescence for the diagnosis of Lassa fever infection. , 1975, Bulletin of the World Health Organization.

[12]  J. Edman,et al.  California group arboviruses in Florida. Host-vector relations. , 1971, The American journal of tropical medicine and hygiene.