Free radical scavenging activity in in vitro-derived tissues of Eruca sativa

Feasible regeneration protocol for economically important plant Eruca sativa was established and 1, 1-diphenyl-2-picrylhydrazyl scavenging activity of regenerated tissues was evaluated and compared with plant material collected from the wild. Leaf portions inoculated onto Murashige and Skoog (MS) medium responded to all plant growth regulators exploited. Optimum callus production was achieved on a combination of 2.0 mg l−1 6-benzyladenine (BA) + 1.0 mg l−1 α-naphthalene acetic acid (NAA) and the lowest response was recorded for 0.5 mg l−1 gibberellic acid (GA3) + 1.0 mg l−1 NAA. The callus was subcultured on similar composition/concentrations of plant growth regulators after 4 weeks of culture time. A 5.0 mg l−1 6-BA + 1.0 mg l−1 NAA produced optimum percentage shoot organogenesis after 4 weeks of subculturing. However, optimum number of shoots per explant was recorded for moderate concentrations (1.0 and 2.0 mg l−1) of kinetin. Incorporation of NAA into MS medium-containing GA3 also produced a feasible number of shoots/explant. Similar mean shoot length was recorded for 2.0 mg l−1 kinetin + 1.0 mg l−1 NAA and optimum concentrations (2.0, 5.0, and 10.0 mg l−1) of GA3 + 1.0 mg l−1 NAA. In vitro generated shoots were shifted to MS medium augmented with indole acetic acid (IAA) for rooting after 4 weeks of subculturing. Moderate concentrations (5.0 mg l−1) of IAA produced feasible rooting. Investigation of radical scavenging activity showed that callus possesses higher levels of radical scavengers than other plant tissues tested. Phenolics and glucosides are reported to be active components of Eruca sativa phytochemistry.

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