A semester-long student-directed research project involving enzyme immunoassay: appropriate for immunology, endocrinology, or neuroscience courses.

The following project aimed at promoting integrated and long-lasting learning is described for an Immunology course, but it may be adapted to other disciplines. Students were asked to develop and carry out a research project to examine the relationship between immune function and stress. The experiments were required to include the assessment of salivary cortisol and salivary IgA (sIgA) with enzyme immunoassays. All other aspects of the experiments were developed by student groups with appropriate guidance from the instructor. Data are presented for one group project that assessed the effect of music on cortisol and sIgA. Overall levels of sIgA and cortisol were consistent with reported values. Students found a significant decrease in cortisol over time. Additionally, there was a trend that supported the overall student hypothesis regarding the effect of stress and immune function. Compared with the same Immunology course that included an instructor-designed experiment using enzyme immunoassays for cortisol and sIgA, several assessments (e.g., final grades and comments on student evaluations) show that overall learning seemed to be much better in the course with the student-directed research project.

[1]  J. Bransford.  How people learn , 2000 .

[2]  Robert M. Sapolsky,et al.  Why zebras don't get ulcers : an updated guide to stress, stress-related diseases, and coping , 1994 .

[3]  Shigeki Watanuki,et al.  Physiological responses induced by pleasant stimuli. , 2005, Journal of physiological anthropology and applied human science.

[4]  M. Sundberg,et al.  Assessing student learning. , 2002, Cell biology education.

[5]  L. Cahill,et al.  Enhanced human memory consolidation with post-learning stress: interaction with the degree of arousal at encoding. , 2003, Learning & memory.

[6]  Jann E. Freed,et al.  Learner-Centered Assessment on College Campuses: Shifting the Focus from Teaching to Learning , 1999 .

[7]  Sarah McClusky Walleck.  Thinking about Teaching and Learning: Developing Habits of Learning with First Year College and University Students , 2005 .

[8]  L. Strazdins,et al.  Impact of saliva collection methods on sIgA and cortisol assays and acceptability to participants. , 2005, Journal of immunological methods.

[9]  S. Watanuki,et al.  Specific physiological responses in women with severe primary dysmenorrhea during the menstrual cycle. , 2005, Journal of physiological anthropology and applied human science.

[10]  G. J. Walker,et al.  Salivary IgA responses to prolonged intensive exercise following caffeine ingestion. , 2006, Medicine and science in sports and exercise.

[11]  D. Koh,et al.  Salivary biomarkers associated with academic assessment stress among dental undergraduates. , 2003, Journal of dental education.

[12]  Ann L. Brown,et al.  How people learn: Brain, mind, experience, and school. , 1999 .

[13]  A. Hobson.  Teaching Relevant Science for Scientific Literacy: Adding Cultural Context to the Sciences. , 2001 .

[14]  F. Dhabhar,et al.  Stress-induced enhancement of antigen-specific cell-mediated immunity. , 1996, Journal of immunology.

[15]  E. Lynd-Balta.  Using literature and innovative assessments to ignite interest and cultivate critical thinking skills in an undergraduate neuroscience course. , 2006, CBE - Life Sciences Education.

[16]  Rudolph Mitchell,et al.  A small-scale concept-based laboratory component: the best of both worlds. , 2006, CBE life sciences education.

[17]  William B Wood,et al.  Teaching more by lecturing less. , 2005, Cell biology education.

[18]  Jennifer L. Chiu,et al.  Teaching and Assessing Knowledge Integration in Science , 2006, Science.

[19]  Diane Ebert-May,et al.  Scientific Teaching , 2004, Science.

[20]  G. Crabtree,et al.  Negative regulation of interleukin 2 transcription by the glucocorticoid receptor , 1992, The Journal of experimental medicine.

[21]  R. Felder,et al.  A Longitudinal Study of Engineering Student Performance and Retention , 1992, Proceedings. Twenty-Second Annual conference Frontiers in Education.

[22]  R. Felder,et al.  Navigating the Bumpy Road to Student-Centered Instruction , 1996 .

[23]  F. Dhabhar,et al.  Acute Stress Enhances while Chronic Stress Suppresses Cell-Mediated Immunityin Vivo:A Potential Role for Leukocyte Trafficking , 1997, Brain, Behavior, and Immunity.

[24]  L. Liotta,et al.  Organic Chemistry of the Cell: An Interdisciplinary Approach To Learning with a Focus on Reading, Analyzing, and Critiquing Primary Literature , 2005 .

[25]  Gary E. Jones,et al.  The Impact of Abbreviated Progressive Muscle Relaxation on Salivary Cortisol and Salivary Immunoglobulin A (sIgA) , 2005, Applied psychophysiology and biofeedback.

[26]  Richard M. Felder,et al.  Beating The Numbers Game: Effective Teaching In Large Classes , 1997 .

[27]  Toni,et al.  An End to Stress , 2006 .

[28]  M. Butovskaya,et al.  The hormonal basis of reconciliation in humans. , 2005, Journal of physiological anthropology and applied human science.

[29]  C. Hansel.  Scientific Teaching , 1972, Nature.

[30]  Robert N. Leamnson.  Thinking About Teaching and Learning: Developing Habits of Learning with First Year College and University Students , 1999 .

[31]  Philip M. Sadler,et al.  Factors Influencing College Science Success. , 2006 .

[32]  I. Screpanti,et al.  Glucocorticoid receptor-mediated suppression of the interleukin 2 gene expression through impairment of the cooperativity between nuclear factor of activated T cells and AP-1 enhancer elements , 1992, The Journal of experimental medicine.

[33]  Richard M. Felder,et al.  A Longitudinal Study of Engineering Student Performance and Retention. IV. Instructional Methods , 1995 .

[34]  A. Lawson.  Points of view: on the implications of neuroscience research for science teaching and learning: are there any? , 2006, CBE life sciences education.