Performances and Biosensing Mechanisms of Interdigitated Capacitive Sensors Based on the Hetero-mixture of SnO2 and In2O3

This study aims to discuss the synthesis and fabrication of SnO2-In2O3-based thick-films and their biosensing applications. The structural characterization of SnO2-In2O3 nanocomposites was performed using X-ray diffraction, Raman spectroscopy and transmission electron microscopy. Furthermore, the screen-printing technology was used in the fabrication of conductive electrodes to form an interdigitated capacitive structure, and the sensor layer based on the mixture of SnO2 and In2O3. Moreover, the sensing performance of the developed structure was tested using Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) bacteria. In addition, the validation of sensing characteristics was performed by electrochemical impedance spectroscopic and self-resonant frequency analysis. Finally, the sensing properties were analyzed for two consecutive days, and changes in both P. aeruginosa and S. aureus pathogens growing media were also studied.

[1]  K. Yoo,et al.  Nano-grained thin-film indium tin oxide gas sensors for H2 detection , 2005 .

[2]  L. Francioso,et al.  Chemical Synthesis, Characterization and Gas-Sensing Properties of Thin Films in the In 2 O 3 -SnO 2 System , 2004 .

[3]  N. Uria,et al.  Impedimetric Sensors for Bacteria Detection , 2015 .

[4]  G. Korotcenkov,et al.  The Role of Grain Size in Response of SnO2- and In2O3-Based Conductometric Gas Sensors , 2012 .

[5]  B. Buszewski,et al.  The effect of growth medium on an Escherichia coli pathway mirrored into GC/MS profiles , 2017, Journal of breath research.

[6]  B. D. Malhotra,et al.  An impedimetric biosensor based on electrophoretically assembled ZnO nanorods and carboxylated graphene nanoflakes on an indium tin oxide electrode for detection of the DNA of Escherichia coli O157:H7 , 2019, Microchimica Acta.

[7]  B. Buszewski,et al.  The effect of biosilver nanoparticles on different bacterial strains’ metabolism reflected in their VOCs profiles , 2018, Journal of breath research.

[8]  J. Ying,et al.  SnO2−In2O3 Nanocomposites as Semiconductor Gas Sensors for CO and NOx Detection , 2007 .

[9]  O. Ilegbusi,et al.  Effect of interaction between components of In2O3-CeO2 and SnO2-CeO2 nanocomposites on structure and sensing properties , 2019, Sensors and Actuators B: Chemical.

[10]  A. Driessen,et al.  Translocation of proteins across the cell envelope of Gram-positive bacteria. , 2001, FEMS microbiology reviews.

[11]  Zhenyu Feng,et al.  In2O3-SnO2 hybrid porous nanostructures delivering enhanced formaldehyde sensing performance , 2018 .

[12]  Shouli Bai,et al.  Preparation, characterization and gas-sensing properties of SnO2-In2O3 nanocomposite oxides , 2006 .

[13]  David T. Limmer,et al.  Concentration Fluctuations and Capacitive Response in Dense Ionic Solutions. , 2016, The journal of physical chemistry letters.

[14]  N. Jaffrezic‐Renault,et al.  Electrochemical impedance immunosensor for rapid detection of stressed pathogenic Staphylococcus aureus bacteria , 2015, Environmental Science and Pollution Research.

[15]  Kuang He,et al.  Gas-Sensing Performances of Metal Oxide Nanostructures for Detecting Dissolved Gases: A Mini Review , 2020, Frontiers in Chemistry.

[16]  Krzysztof Zaraska,et al.  Electrochemical Impedance Spectroscopic Analysis of RuO2 Based Thick Film pH Sensors , 2015 .

[17]  Ghenadii Korotcenkov,et al.  In2O3- and SnO2-based Ozone Sensors: Design and Characterization , 2018 .

[18]  Ghenadii Korotcenkov,et al.  In2O3- and SnO2-Based Thin Film Ozone Sensors: Fundamentals , 2016, J. Sensors.

[19]  I. Ratiu,et al.  Sensors' array of aspiration ion mobility spectrometer as a tool for bacteria discrimination. , 2020, Talanta.

[20]  A. Patrut,et al.  Discrimination of bacteria by rapid sensing their metabolic volatiles using an aspiration-type ion mobility spectrometer (a-IMS) and gas chromatography-mass spectrometry GC-MS. , 2017, Analytica chimica acta.

[21]  A. Lasia Electrochemical Impedance Spectroscopy and its Applications , 2014 .

[22]  T. Silhavy,et al.  The bacterial cell envelope. , 2010, Cold Spring Harbor perspectives in biology.

[23]  Samantha E. McBirney,et al.  Wavelength-normalized spectroscopic analysis of Staphylococcus aureus and Pseudomonas aeruginosa growth rates. , 2016, Biomedical optics express.

[24]  Ghenadii Korotcenkov,et al.  Grain Size Effects in Sensor Response of Nanostructured SnO2- and In2O3-Based Conductometric Thin Film Gas Sensor , 2009 .

[25]  Juewen Liu,et al.  Sensors and biosensors based on metal oxide nanomaterials , 2019 .

[26]  D. E. Yates,et al.  Site-binding model of the electrical double layer at the oxide/water interface , 1974 .

[27]  Ludovic S. Live,et al.  Solution-based circuits enable rapid and multiplexed pathogen detection , 2013, Nature Communications.

[28]  Jiangjiang Zhu,et al.  Fast Detection of Volatile Organic Compounds from Bacterial Cultures by Secondary Electrospray Ionization-Mass Spectrometry , 2010, Journal of Clinical Microbiology.

[29]  G. Gomila,et al.  Electric polarization properties of single bacteria measured with electrostatic force microscopy. , 2014, ACS nano.

[30]  R. Compton,et al.  Electrochemical Detection of Pathogenic Bacteria-Recent Strategies, Advances and Challenges. , 2018, Chemistry, an Asian journal.

[31]  I. Ratiu,et al.  Discrimination of Chemical Profiles of Some Bacterial Species by Analyzing Culture Headspace Air Samples Using TD-GC/MS , 2014 .

[32]  N. S. Goloborodko,et al.  Optical and Electrophysical Properties of 95% In2O3 + 5% SnO2/ns-Si Heterostructure , 2016 .

[33]  T. V. Belysheva,et al.  The sensor properties of SnO2 · In2O3 nanocomposite oxides in the detection of hydrogen in air , 2010 .

[34]  Yude Wang,et al.  Nonaqueous synthesis of Pd-functionalized SnO2/In2O3 nanocomposites for excellent butane sensing properties , 2018 .

[35]  Hossam Haick,et al.  Advanced Materials for Health Monitoring with Skin‐Based Wearable Devices , 2017, Advanced healthcare materials.

[36]  R. Firstenberg-Eden,et al.  Electrochemical changes in media due to microbial , 1984 .

[37]  P. Kurzweil,et al.  Metal Oxides and Ion-Exchanging Surfaces as pH Sensors in Liquids: State-of-the-Art and Outlook , 2009, Sensors.

[38]  Dapeng Xu,et al.  Preparation of one-dimensional SnO2–In2O3 nano-heterostructures and their gas-sensing property , 2017 .

[39]  Liang Kaiming,et al.  Gas-Sensing Performances of Metal Oxide Nanostructures for Detecting Dissolved Gases: A Mini Review. , 2020 .

[40]  J. V. Van Impe,et al.  Protein secretion biotechnology in Gram-positive bacteria with special emphasis on Streptomyces lividans. , 2014, Biochimica et biophysica acta.