Rapid nucleic acid detection of Zaire ebolavirus on paper fluidics

We described a novel system of paper fluidics, in which isothermal nucleic acid amplification and detection are performed. This paper system made the nucleic acid test simpler, easier to operate, cheap, and provided the performance of point-of-care testing. This system was used to detect the ebolavirus rapidly with high specificity and sensitivity.

[1]  Yong-Jin Yoon,et al.  Rapid and label-free amplification and detection assay for genotyping of cancer biomarker. , 2015, Biosensors & bioelectronics.

[2]  Radoje Drmanac,et al.  Multiplexed SNP genotyping using nanobarcode particle technology , 2006, Analytical and bioanalytical chemistry.

[3]  Ali Kemal Yetisen,et al.  Paper-based microfluidic point-of-care diagnostic devices. , 2013, Lab on a chip.

[4]  Jeong-O Lee,et al.  Detection and titer estimation of Escherichia coli using aptamer-functionalized single-walled carbon-nanotube field-effect transistors. , 2008, Small.

[5]  Y. Mori,et al.  Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. , 2001, Biochemical and biophysical research communications.

[6]  M. Aman,et al.  Determination of Specific Antibody Responses to the Six Species of Ebola and Marburg Viruses by Multiplexed Protein Microarrays , 2014, Clinical and Vaccine Immunology.

[7]  Y. Mori,et al.  Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases , 2009, Journal of Infection and Chemotherapy.

[8]  Y. Kawaoka,et al.  Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification. , 2007, Journal of virological methods.

[9]  Randy L. Gollub,et al.  Associations between DNA methylation and schizophrenia-related intermediate phenotypes — A gene set enrichment analysis , 2015, Progress in Neuro-Psychopharmacology and Biological Psychiatry.

[10]  P. Gill,et al.  Nucleic Acid Isothermal Amplification Technologies—A Review , 2008, Nucleosides, nucleotides & nucleic acids.

[11]  R S Foote,et al.  Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing. , 1998, Analytical chemistry.

[12]  G. Whitesides,et al.  Three-dimensional microfluidic devices fabricated in layered paper and tape , 2008, Proceedings of the National Academy of Sciences.

[13]  Yi Guan,et al.  Detection of Human Influenza A Viruses by Loop-Mediated Isothermal Amplification , 2005, Journal of Clinical Microbiology.

[14]  Chantal Reusken,et al.  Identification of essential outstanding questions for an adequate European laboratory response to Ebolavirus Zaire West Africa 2014. , 2015, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.

[15]  Mohammadali Safavieh,et al.  A simple cassette as point-of-care diagnostic device for naked-eye colorimetric bacteria detection. , 2014, The Analyst.

[16]  Shinji Watanabe,et al.  Characterization of Ebolavirus regulatory genomic regions. , 2009, Virus research.

[17]  M. Tabrizian,et al.  Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics. , 2012, Lab on a chip.

[18]  Shasha Wei,et al.  Paper-based microfluidics with high resolution, cut on a glass fiber membrane for bioassays. , 2014, Lab on a chip.

[19]  Jim Carney,et al.  Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Point-of-Care Device for Detection of Herpes Simplex Virus Type 2-Specific Immunoglobulin G Antibodies in Serum and Whole Blood , 2007, Clinical and Vaccine Immunology.

[20]  I. Wilson,et al.  Ebolavirus VP35 Coats the Backbone of Double-Stranded RNA for Interferon Antagonism , 2013, Journal of Virology.

[21]  Christopher L. Cassano,et al.  Laminated paper-based analytical devices (LPAD): fabrication, characterization, and assays , 2013 .