Monolithic integration of poly(dimethylsiloxane) waveguides and microfluidics for on-chip absorbance measurements

Abstract A monolithic integration of poly(dimethylsiloxane) (PDMS) waveguides and microfluidics is demonstrated by implementing a two-step lithographic moulding. Silicone oil is added in PDMS precursor to tune the refractive index of waveguides with air as the side and top cladding layer. Curved and straight waveguides are characterized for their insertion loss mainly due to propagation, radiation, and transition. Transition and radiation losses are identified dominating along the curves whereas propagation loss increases at a steady rate of 0.249 ± 0.015 dB/cm. With the integrated system, real-time absorbance measurement of fluorescein streams yields a nearly linear response up to 250 μM and the limit of detection

[1]  Hans Peter Herzig,et al.  Performance of an integrated microoptical system for fluorescence detection in microfluidic systems. , 2002, Analytical chemistry.

[2]  A. Llobera,et al.  Simple estimation of transition losses in bends of wide optical waveguides by a ray tracing method , 2004, IEEE Photonics Technology Letters.

[3]  K. Mogensen,et al.  Performance of an in‐plane detection cell with integrated waveguides for UV/Vis absorbance measurements on microfluidic separation devices , 2002, Electrophoresis.

[4]  Bryan G. Splawn,et al.  On-chip absorption measurements using an integrated waveguide , 2002, Analytical and bioanalytical chemistry.

[5]  Ioannis Papakonstantinou,et al.  Transition, radiation and propagation loss in polymer multimode waveguide bends. , 2007, Optics express.

[6]  K. Mayora,et al.  A new SU-8 process to integrate buried waveguides and sealed microchannels for a Lab-on-a-Chip , 2006 .

[7]  J. Eijkel,et al.  A microfluidic device with an integrated waveguide beam splitter for velocity measurements of flowing particles by Fourier transformation. , 2003, Analytical chemistry.

[8]  Joseph T. Boyd,et al.  Measurement of mode field profiles and bending and transition losses in curved optical channel waveguides , 1997 .

[9]  Detlef Snakenborg,et al.  Direct milling and casting of polymer-based optical waveguides for improved transparency in the visible range , 2006 .

[10]  Joseba Zubia,et al.  Mode coupling contribution to radiation losses in curvatures for high and low numerical aperture plastic optical fibers , 2002 .

[11]  K. Mogensen,et al.  Integration of polymer waveguides for optical detection in microfabricated chemical analysis systems. , 2003, Applied optics.

[12]  D. J. Harrison,et al.  Microfabrication of a Planar Absorbance and Fluorescence Cell for Integrated Capillary Electrophoresis Devices , 1996 .

[13]  B. Gale,et al.  A monolithic PDMS waveguide system fabricated using soft-lithography techniques , 2005, Journal of Lightwave Technology.

[14]  D. Bornhop,et al.  Detection in capillary electrophoresis , 2000, Electrophoresis.

[15]  H Fujita,et al.  PDMS 2D optical lens integrated with microfluidic channels: principle and characterization. , 2003, Lab on a chip.

[16]  E. Verpoorte.  Chip vision-optics for microchips. , 2003, Lab on a chip.

[17]  K. Mogensen,et al.  Recent developments in detection for microfluidic systems , 2004, Electrophoresis.

[18]  Jörg P Kutter,et al.  Pure‐silica optical waveguides, fiber couplers, and high‐aspect ratio submicrometer channels for electrokinetic separation devices , 2004, Electrophoresis.

[19]  Edmond Cambril,et al.  On-chip optical components and microfluidic systems , 2004 .

[20]  Yi Zhang,et al.  An integrated fluorescence detection system for lab-on-a-chip applications. , 2007, Lab on a chip.

[22]  K. Mogensen,et al.  Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements. , 2004, Lab on a chip.

[23]  G. Whitesides,et al.  Dynamic control of liquid-core/liquid-cladding optical waveguides , 2004, (CLEO). Conference on Lasers and Electro-Optics, 2005..

[24]  S Büttgenbach,et al.  Poly(dimethylsiloxane) hollow Abbe prism with microlenses for detection based on absorption and refractive index shift. , 2004, Lab on a chip.

[25]  G. Whitesides,et al.  Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies , 2003, Electrophoresis.

[26]  A. Driessen,et al.  Low-cost and low-loss multimode waveguides of photodefinable epoxy , 2006, IEEE Photonics Technology Letters.

[27]  Paul Lambeck,et al.  Highly-sensitive passive integrated optical spiral-shaped waveguide refractometer , 1997 .

[28]  D. Bradley,et al.  Monolithically integrated dye-doped PDMS long-pass filters for disposable on-chip fluorescence detection. , 2006, Lab on a chip.

[29]  Stefan Kopetz,et al.  PDMS-based optical waveguide layer for integration in electrical–optical circuit boards , 2007 .

[30]  Nickolaj J. Petersen,et al.  Monolithic integration of optical waveguides for absorbance detection in microfabricated electrophoresis devices , 2001, Electrophoresis.

[31]  W. Chang,et al.  Fabrication of polymer waveguides by a replication method. , 2006, Applied optics.

[32]  Bruce K Gale,et al.  An integrated optical oxygen sensor fabricated using rapid-prototyping techniques. , 2003, Lab on a chip.