论文信息 - Maintenance of the in vitro storage properties of apheresis platelets suspended in PAS‐F after a 24‐hour interruption of agitation

Maintenance of the in vitro storage properties of apheresis platelets suspended in PAS‐F after a 24‐hour interruption of agitation

During shipment of platelets (PLTs), the continuous agitation required to maintain PLT pH and other storage properties is not possible. In vitro studies can be useful in evaluating the maintenance of PLT quality variables after an interruption of agitation designed to mimic PLT shipment. A previous study demonstrated that Amicus (Fenwal, Inc., Lake Zurich, IL) and Trima apheresis (Terumo BCT, Lakewood, CO) PLTs suspended in 100% plasma can maintain pH values higher than 6.2 during 5-day storage with either 24or 30-hour periods of interrupted agitation. A subsequent study reported that PLTs collected on the Amicus separator and resuspended in 65% PAS-C (PAS-III) with 35% plasma can maintain pH higher than 6.2 during 5-day storage with a 24-hour interruption of agitation. The in vitro properties and in vivo recovery and survival of PLTs collected on the Trima Accel containing 65% of PAS-F (Isoplate) and 35% plasma have been reported; this product was cleared by the Food and Drug Administration on March 5, 2013. This study evaluates the in vitro properties of Trima PLTs containing 65% PAS-F and 35% plasma subjected to a 24-hr period without agitation during 5 days of storage. A hyperconcentrated double PLT Trima apheresis product was collected (n 5 12) with a targeted total content of 7.0 3 10 PLTs; the volume was divided to contain two identical units. To one unit (control) concurrent plasma was added to yield a product in 100% plasma. To the other unit (test) PAS-F was added to yield a suspension containing 65% PAS-F and 35% plasma. The two units were stored at 20 to 24 C with reciprocal agitation. On the morning of Day 2, both units were removed from the agitator and placed in a stationary shipping container with temperature stabilizing packs at 20 to 24 C for 24 6 2 hours. After the interruption of agitation, both units were stored at 20 to 24 C with agitation. Test and control PLTs were sampled on Day 2 before interruption of agitation, Day 3 after an interruption of agitation, and on the morning of Day 6

S. Wagner | S. Seetharaman | Tanya Cook

[1] S. Wagner,et al. An Inhibition of p38 Mitogen Activated Protein Kinase Delays the Platelet Storage Lesion , 2013, PLoS ONE.

[2] J. Cancelas,et al. In vitro and in vivo quality of leukoreduced apheresis platelets stored in a new platelet additive solution , 2013, Transfusion.

[3] S. Wagner,et al. Comparative in vitro evaluation of apheresis platelets stored with 100% plasma or 65% platelet additive solution III/35% plasma and including periods without agitation under simulated shipping conditions , 2012, Transfusion.

[4] S. Wagner,et al. Comparison of the in vitro properties of apheresis platelets during 7‐day storage after interrupting agitation for one or three periods , 2008, Transfusion.