论文信息 - APPLICATION OF IN-SITU-MICROSCOPY AND DIGITAL IMAGE PROCESSING IN YEAST CULTIVATIONS

APPLICATION OF IN-SITU-MICROSCOPY AND DIGITAL IMAGE PROCESSING IN YEAST CULTIVATIONS

Abstract In-situ-microscopy is an efficient method to measure cell concentration during cultivations. Furthermore the size distribution of the cells as well as their morphology can be determined on-line. To operate the microscope and acquire sequences of cell images a software system has been developed. This software also allows the on-line analysis of the image data to determine the cell concentration. The software, the image analysis algorithm as well as the analysis results of a yeast cell cultivation are presented. It is demonstrated, that the automated determination of the cell numbers agree very well with its manual count.

Bernd Hitzmann | Patrick Lindner | C. Krabichler | G. Rudolph | Th. Scheper

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