Flash microbiocide: A Rapid and Economic Method for Determination of MBC and MFC

Although nowadays there are methods for determining the Minimal Bactericidal Concentration and Minimal Fungicidal Concentration, it is indispensable that the establishment of innovative methodologies could be more practical and cheaper. The new methodology Flash microbiocide is an assay in which one aliquot from 96 well plate of Minimal Inhibitory Concentration test is transferred to another plate containing different culture medium. The correspondence with the reference methods described in the National Committee for Clinical Laboratory Standards (NCCLS-CLSI) document M26-A was achieved, denoting the efficiency of this fast and simple method.

[1]  Clinical,et al.  Reference method for broth dilution antifungal susceptibility testing of filamentous fungi : Approved standard , 2008 .

[2]  Clinical,et al.  Reference method for broth dilution antifungal susceptibility testing of yeasts : Approved standard , 2008 .

[3]  L. Sabath,et al.  Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections. , 2004, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[4]  Mary Jane Ferraro,et al.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically : approved standard , 2000 .

[5]  J. Waitz.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically , 1990 .

[6]  A. Tomasz,et al.  Antibiotic tolerance among clinical isolates of bacteria , 1986, Antimicrobial Agents and Chemotherapy.

[7]  J. Sculier,et al.  Significance of serum bactericidal activity in gram-negative bacillary bacteremia in patients with and without granulocytopenia. , 1984, The American journal of medicine.

[8]  J. Dankert,et al.  Importance of minimizing carry-over effect at subculture in the detection of penicillin-tolerant viridans group streptococci , 1983, Antimicrobial Agents and Chemotherapy.

[9]  M. Sande,et al.  Bactericidal versus bacteriostatic antibiotic therapy of experimental pneumococcal meningitis in rabbits. , 1983, The Journal of clinical investigation.

[10]  A. Barry,et al.  In-vitro methods for determining minimal lethal concentrations of antimicrobial agents. , 1979, American journal of clinical pathology.