Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

[1]  Simon P. Shen,et al.  Engineered pegRNAs improve prime editing efficiency , 2021, Nature Biotechnology.

[2]  Jeffrey A. Hussmann,et al.  Mapping the genetic landscape of DNA double-strand break repair , 2021, Cell.

[3]  J. Seong,et al.  Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor , 2021, Genome biology.

[4]  Kunling Chen,et al.  Genome-wide specificity of prime editors in plants , 2021, Nature Biotechnology.

[5]  Jonathan Y. Hsu,et al.  CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells , 2021, Nature Biotechnology.

[6]  D. Lavery,et al.  Generation and characterization of human induced pluripotent stem cells (iPSCs) from three male and three female patients with CDKL5 Deficiency Disorder (CDD). , 2021, Stem cell research.

[7]  Katherine R. Smith,et al.  A systematic CRISPR screen defines mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage , 2021, Nature Cancer.

[8]  T. Flotte,et al.  Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice , 2020, Nature Communications.

[9]  Yong-Sam Kim,et al.  Unbiased investigation of specificities of prime editing systems in human cells , 2020, Nucleic acids research.

[10]  N. Perrimon,et al.  Precise genome engineering in Drosophila using prime editing , 2020, Proceedings of the National Academy of Sciences.

[11]  David R. Liu,et al.  Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors , 2020, Nature Biotechnology.

[12]  M. Mokry,et al.  Prime editing for functional repair in patient-derived disease models , 2020, Nature Communications.

[13]  F. Buchholz,et al.  Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors , 2020, Genes.

[14]  Yulin Chen,et al.  Efficient generation of mouse models with the prime editing system , 2020, Cell Discovery.

[15]  David R. Liu,et al.  Prime genome editing in rice and wheat , 2020, Nature Biotechnology.

[16]  David R. Liu,et al.  Search-and-replace genome editing without double-strand breaks or donor DNA , 2019, Nature.

[17]  E. Pestana-Knight,et al.  Cyclin-Dependent Kinase-Like 5 Deficiency Disorder: Clinical Review. , 2019, Pediatric neurology.

[18]  Matthew C. Canver,et al.  CRISPResso2 provides accurate and rapid genome editing sequence analysis , 2019, Nature Biotechnology.

[19]  D. Guo,et al.  HIV-1 inhibition in cells with CXCR4 mutant genome created by CRISPR-Cas9 and piggyBac recombinant technologies , 2018, Scientific Reports.

[20]  David Baker,et al.  Selective targeting of engineered T cells using orthogonal IL-2 cytokine-receptor complexes , 2018, Science.

[21]  Xiaoliu Zhang,et al.  Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains , 2017, Scientific Reports.

[22]  J. Joung,et al.  CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR-Cas9 nuclease off-targets , 2017, Nature Methods.

[23]  Sheena M. Scroggins,et al.  MSIplus for Integrated Colorectal Cancer Molecular Testing by Next-Generation Sequencing. , 2015, The Journal of molecular diagnostics : JMD.

[24]  Morten H. H. Nørholm,et al.  Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning. , 2015, ACS synthetic biology.

[25]  Sebastian Brandner,et al.  A naturally occurring variant of the human prion protein completely prevents prion disease , 2015, Nature.

[26]  David A. Scott,et al.  In vivo genome editing using Staphylococcus aureus Cas9 , 2015, Nature.

[27]  Ben Lehner,et al.  Differential DNA mismatch repair underlies mutation rate variation across the human genome , 2015, Nature.

[28]  T. Kunkel,et al.  Heterogeneous polymerase fidelity and mismatch repair bias genome variation and composition , 2014, Genome research.

[29]  Max A. Horlbeck,et al.  Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation , 2014, Cell.

[30]  S. Cantor,et al.  Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses , 2014, The EMBO journal.

[31]  P. Grisanti,et al.  Interpreting elevated fetal hemoglobin in pathology and health at the basic laboratory level: new and known γ‐ gene mutations associated with hereditary persistence of fetal hemoglobin , 2014, International journal of laboratory hematology.

[32]  Luke A. Gilbert,et al.  CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes , 2013, Cell.

[33]  B. Gilquin,et al.  Structure of the MutLα C-terminal domain reveals how Mlh1 contributes to Pms1 endonuclease site , 2013, Nature Structural &Molecular Biology.

[34]  Wei Yang,et al.  Mechanism of mismatch recognition revealed by human MutSβ bound to unpaired DNA loops , 2011, Nature Structural &Molecular Biology.

[35]  P. Hsieh,et al.  In vitro studies of DNA mismatch repair proteins. , 2011, Analytical biochemistry.

[36]  P. Modrich,et al.  PCNA function in the activation and strand direction of MutLα endonuclease in mismatch repair , 2010, Proceedings of the National Academy of Sciences.

[37]  Claudio J. Verzilli,et al.  A novel protective prion protein variant that colocalizes with kuru exposure. , 2009, The New England journal of medicine.

[38]  A. Corbett,et al.  The intracellular mobility of nuclear import receptors and NLS cargoes. , 2009, Biophysical journal.

[39]  Carola Engler,et al.  A One Pot, One Step, Precision Cloning Method with High Throughput Capability , 2008, PloS one.

[40]  Guo-Min Li,et al.  Mechanisms and functions of DNA mismatch repair , 2008, Cell Research.

[41]  L. Beese,et al.  Structure of the Human MutSα DNA Lesion Recognition Complex , 2007 .

[42]  P. Modrich,et al.  Endonucleolytic Function of MutLα in Human Mismatch Repair , 2006, Cell.

[43]  P. Modrich,et al.  DNA mismatch repair: functions and mechanisms. , 2006, Chemical reviews.

[44]  Mark E. Davis,et al.  Insights into the kinetics of siRNA-mediated gene silencing from live-cell and live-animal bioluminescent imaging , 2006, Nucleic acids research.

[45]  A. Tomkinson,et al.  Reconstitution of 5′-Directed Human Mismatch Repair in a Purified System , 2005, Cell.

[46]  T. Kunkel,et al.  DNA mismatch repair. , 2005, Annual review of biochemistry.

[47]  A. Tomkinson,et al.  Human DNA ligase I completely encircles and partially unwinds nicked DNA , 2004, Nature.

[48]  Lawrence J. Burgart,et al.  Development of a Fluorescent Multiplex Assay for Detection of MSI-High Tumors , 2004, Disease markers.

[49]  James E Haber,et al.  Heteroduplex rejection during single-strand annealing requires Sgs1 helicase and mismatch repair proteins Msh2 and Msh6 but not Pms1. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[50]  R. Bambara,et al.  Flap endonuclease 1: a central component of DNA metabolism. , 2004, Annual review of biochemistry.

[51]  Sudhir Srivastava,et al.  Revised Bethesda Guidelines for hereditary nonpolyposis colorectal cancer (Lynch syndrome) and microsatellite instability. , 2004, Journal of the National Cancer Institute.

[52]  J. Trojan,et al.  N‐terminus of hMLH1 confers interaction of hMutLα and hMutLβ with hMutSα , 2003 .

[53]  A. Hall,et al.  Assessment of mismatch repair function in leukaemic cell lines and blasts from children with acute lymphoblastic leukaemia. , 2003, Carcinogenesis.

[54]  R. Liskay,et al.  Contribution of Human Mlh1 and Pms2 ATPase Activities to DNA Mismatch Repair* , 2002, The Journal of Biological Chemistry.

[55]  J. Jiricny,et al.  Mutations within the hMLH1 and hPMS2 Subunits of the Human MutLα Mismatch Repair Factor Affect Its ATPase Activity, but Not Its Ability to Interact with hMutSα* , 2002, The Journal of Biological Chemistry.

[56]  Paul Modrich,et al.  Human Exonuclease I Is Required for 5′ and 3′ Mismatch Repair* , 2002, The Journal of Biological Chemistry.

[57]  J. Jiricny,et al.  Functional analysis of hMLH1 variants and HNPCC-related mutations using a human expression system. , 2002, Gastroenterology.

[58]  M. Hung,et al.  Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells , 2001, Nature Cell Biology.

[59]  R. Fishel,et al.  The Interaction of the Human MutL Homologues in Hereditary Nonpolyposis Colon Cancer* , 1999, The Journal of Biological Chemistry.

[60]  Paul Modrich,et al.  Isolation of MutSβ from Human Cells and Comparison of the Mismatch Repair Specificities of MutSβ and MutSα* , 1998, The Journal of Biological Chemistry.

[61]  J. Jiricny,et al.  hMSH2 and hMSH6 play distinct roles in mismatch binding and contribute differently to the ATPase activity of hMutSα , 1998, The EMBO journal.

[62]  D. Gordenin,et al.  Hypermutability of homonucleotide runs in mismatch repair and DNA polymerase proofreading yeast mutants , 1997, Molecular and cellular biology.

[63]  G. Marsischky,et al.  hMSH2 forms specific mispair-binding complexes with hMSH3 and hMSH6. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[64]  T. Kunkel,et al.  DNA loop repair by human cell extracts. , 1994, Science.

[65]  Bert Vogelstein,et al.  Hypermutability and mismatch repair deficiency in RER+ tumor cells , 1993, Cell.

[66]  Tomas A. Prolla,et al.  Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair , 1993, Nature.

[67]  W. Fang,et al.  Human strand-specific mismatch repair occurs by a bidirectional mechanism similar to that of the bacterial reaction. , 1993, The Journal of biological chemistry.

[68]  T. Kunkel,et al.  Heteroduplex repair in extracts of human HeLa cells. , 1991, The Journal of biological chemistry.

[69]  P. Modrich,et al.  Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[70]  P. Modrich,et al.  DNA mismatch correction in a defined system. , 1989, Science.

[71]  C. Dang,et al.  Identification of the human c-myc protein nuclear translocation signal , 1988, Molecular and cellular biology.

[72]  P. Modrich,et al.  Mispair specificity of methyl-directed DNA mismatch correction in vitro. , 1988, The Journal of biological chemistry.

[73]  V. Ingram,et al.  A Specific Chemical Difference Between the Globins of Normal Human and Sickle-Cell Anæmia Hæmoglobin , 1956, Nature.