A difference gel electrophoresis study on thylakoids isolated from poplar leaves reveals a negative impact of ozone exposure on membrane proteins.

Populus tremula L. x P. alba L. (Populus x canescens (Aiton) Smith), clone INRA 717-1-B4, saplings were subjected to 120 ppb ozone exposure for 28 days. Chloroplasts were isolated, and the membrane proteins, solubilized using the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC), were analyzed in a difference gel electrophoresis (DiGE) experiment comparing control versus ozone-exposed plants. Extrinsic photosystem (PS) proteins and adenosine triphosphatase (ATPase) subunits were detected to vary in abundance. The general trend was a decrease in abundance, except for ferredoxin-NADP(+) oxidoreductase (FNR), which increased after the first 7 days of exposure. The up-regulation of FNR would increase NAPDH production for reducing power and detoxification inside and outside of the chloroplast. Later on, FNR and a number of PS and ATPase subunits decrease in abundance. This could be the result of oxidative processes on chloroplast proteins but could also be a way to down-regulate photochemical reactions in response to an inhibition in Calvin cycle activity.

[1]  Wendong Guo,et al.  A systemic proteomic analysis of Populus chloroplast by using shotgun method , 2011, Molecular Biology Reports.

[2]  L. Hoffmann,et al.  Differential impact of chronic ozone exposure on expanding and fully expanded poplar leaves. , 2010, Tree physiology.

[3]  R. Rakwal,et al.  Investigating the impact of elevated levels of ozone on tropical wheat using integrated phenotypical, physiological, biochemical, and proteomics approaches. , 2010, Journal of proteome research.

[4]  S. Komatsu,et al.  Ozone stress‐induced proteomic changes in leaf total soluble and chloroplast proteins of soybean reveal that carbon allocation is involved in adaptation in the early developmental stage , 2010, Proteomics.

[5]  G. Wieser,et al.  Advances in understanding ozone impact on forest trees: messages from novel phytotron and free-air fumigation studies. , 2010, Environmental pollution.

[6]  M. Tamoi,et al.  Arabidopsis chloroplastic ascorbate peroxidase isoenzymes play a dual role in photoprotection and gene regulation under photooxidative stress. , 2010, Plant & cell physiology.

[7]  D. Le Thiec,et al.  Metabolic-dependent changes in plant cell redox power after ozone exposure. , 2009, Plant biology.

[8]  Jenny Renaut,et al.  The impact of atmospheric composition on plants: a case study of ozone and poplar. , 2009, Mass spectrometry reviews.

[9]  L. Hoffmann,et al.  Proteomic and enzymatic response of poplar to cadmium stress. , 2009, Journal of proteomics.

[10]  J. Fuhrer.  Ozone risk for crops and pastures in present and future climates , 2009, Naturwissenschaften.

[11]  D. Le Thiec,et al.  Ozone-induced changes in photosynthesis and photorespiration of hybrid poplar in relation to the developmental stage of the leaves. , 2008, Physiologia plantarum.

[12]  R. Heath.  Modification of the biochemical pathways of plants induced by ozone: what are the varied routes to change? , 2008, Environmental pollution.

[13]  X. Yi,et al.  The effects of simultaneous RNAi suppression of PsbO and PsbP protein expression in photosystem II of Arabidopsis , 2008, Photosynthesis Research.

[14]  Kristie L. Ebi,et al.  Climate Change, Tropospheric Ozone and Particulate Matter, and Health Impacts , 2008, Environmental health perspectives.

[15]  Norio Murata,et al.  How do environmental stresses accelerate photoinhibition? , 2008, Trends in plant science.

[16]  H. Pakrasi,et al.  The Psb27 Protein Facilitates Manganese Cluster Assembly in Photosystem II* , 2008, Journal of Biological Chemistry.

[17]  Panel Intergubernamental sobre Cambio Climático.  Climate change 2007: Synthesis report , 2007 .

[18]  R. Matyssek,et al.  Effects of ozone impact on the gas exchange and chlorophyll fluorescence of juvenile birch stems (Betula pendula Roth.). , 2007, Environmental pollution.

[19]  L. Hoffmann,et al.  A DIGE analysis of developing poplar leaves subjected to ozone reveals major changes in carbon metabolism , 2007, Proteomics.

[20]  Chung-Jui Tsai,et al.  The glycine decarboxylase complex multienzyme family in Populus. , 2007, Journal of experimental botany.

[21]  H. Kaminaka,et al.  Overexpression of monodehydroascorbate reductase in transgenic tobacco confers enhanced tolerance to ozone, salt and polyethylene glycol stresses , 2007, Planta.

[22]  X. Yi,et al.  The PsbQ Protein Is Required in Arabidopsis for Photosystem II Assembly/Stability and Photoautotrophy under Low Light Conditions* , 2006, Journal of Biological Chemistry.

[23]  T. Kieselbach,et al.  The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short- and long-term exposure to low temperature. , 2006, The Plant journal : for cell and molecular biology.

[24]  J. Kangasjärvi,et al.  Signalling and cell death in ozone‐exposed plants , 2005 .

[25]  R. Vingarzan.  A review of surface ozone background levels and trends , 2004 .

[26]  M. Periasamy,et al.  Solubilization of membrane proteins for two-dimensional gel electrophoresis: identification of sarcoplasmic reticulum membrane proteins. , 2004, Analytical biochemistry.

[27]  A. Jönsson,et al.  The use of light and electron microscopy to assess the impact of ozone on Norway spruce needles. , 2004, Environmental pollution.

[28]  R. Scheibe.  Malate valves to balance cellular energy supply. , 2004, Physiologia plantarum.

[29]  Jian-Ren Shen,et al.  Extrinsic proteins of photosystem II: an intermediate member of PsbQ protein family in red algal PS II. , 2003, European journal of biochemistry.

[30]  M. Rousi,et al.  Interactive effect of springtime frost and elevated ozone on early growth, foliar injuries and leaf structure of birch (Betula pendula). , 2003, The New phytologist.

[31]  J. Pushnik,et al.  Chloroplastic responses of ponderosa pine (Pinus ponderosa) seedlings to ozone exposure. , 2003, Environment international.

[32]  W. Chow,et al.  Structural and functional dynamics of plant photosystem II. , 2002, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[33]  M. Knowles,et al.  Alterations of stress related proteins in genetically altered mice revealed by two‐dimensional differential in‐gel electrophoresis analysis , 2002, Proteomics.

[34]  J. Sõber,et al.  Impacts of elevated CO2 and/or O3 on leaf ultrastructure of aspen (Populus tremuloides) and birch (Betula papyrifera) in the aspen FACE experiment. , 2001, Environmental pollution.

[35]  A. Ranieri,et al.  Chronic ozone fumigation induces alterations in thylakoid functionality and composition in two poplar clones , 2001 .

[36]  P. Sétif,et al.  Ferredoxin and flavodoxin reduction by photosystem I. , 2001, Biochimica et biophysica acta.

[37]  P. Fromme,et al.  Structure of photosystem I. , 2001, Biochimica et biophysica acta.

[38]  P. Dizengremel.  Effects of ozone on the carbon metabolism of forest trees , 2001 .

[39]  V. Fontaine,et al.  Changes in Rubisco and Rubisco activase gene expression and polypeptide content in Pinus halepensis M. subjected to ozone and drought , 2001 .

[40]  A. Ranieri,et al.  Differential sensitivity to ozone in two poplar clones. Analysis of thylakoid pigment-protein complexes. , 2000 .

[41]  B. Frey,et al.  Visible and microscopic injury in leaves of five deciduous tree species related to current critical ozone levels. , 2000, Environmental pollution.

[42]  T. Heitz,et al.  Soluble phospholipase A2 activity is induced before oxylipin accumulation in tobacco mosaic virus-infected tobacco leaves and is contributed by patatin-like enzymes. , 2000, The Plant journal : for cell and molecular biology.

[43]  P. Dizengremel,et al.  Beech trees exposed to high CO2 and to simulated summer ozone levels: effects on photosynthesis, chloroplast components and leaf enzyme activity. , 2000 .

[44]  Mary F. Lopez,et al.  Better approaches to finding the needle in a haystack: Optimizing proteome analysis through automation , 2000, Electrophoresis.

[45]  G. Soldatini,et al.  Quenching analysis in poplar clones exposed to ozone. , 1999, Tree physiology.

[46]  K. Asada,et al.  THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons. , 1999, Annual review of plant physiology and plant molecular biology.

[47]  H. Scheller,et al.  The interaction between plastocyanin and photosystem I is inefficient in transgenic Arabidopsis plants lacking the PSI-N subunit of photosystem I. , 1999, The Plant journal : for cell and molecular biology.

[48]  G. Brudvig,et al.  Cytochrome b559 of photosystem II. , 1998, Biochimica et biophysica acta.

[49]  M. S. J. Broadmeadow.  Ozone and forest trees , 1998 .

[50]  E. Pell,et al.  Ozone-induced changes in biosynthesis of Rubisco and associated compensation to stress in foliage of hybrid poplar. , 1998, Tree physiology.

[51]  Richard N. Arteca,et al.  Ozone‐induced oxidative stress: Mechanisms of action and reaction , 1997 .

[52]  J. Barber,et al.  University of Groningen Structure and membrane organization of photosystem II in green plants , 2006 .

[53]  L. Eichacker,et al.  Stabilization of Chlorophyll a-binding Apoproteins P700, CP47, CP43, D2, and D1 by Chlorophyll a or Zn-pheophytin a* , 1996, The Journal of Biological Chemistry.

[54]  H. Neuhaus,et al.  Starch degradation in chloroplasts isolated from C3 or CAM (crassulacean acid metabolism)-induced Mesembryanthemum crystallinum L. , 1996, The Biochemical journal.

[55]  H. Sandermann,et al.  Ozone and plant health. , 1996, Annual review of phytopathology.

[56]  J. Vahala,et al.  Growth responses and related biochemical and ultrastructural changes of the photosynthetic apparatus in birch (Betula pendula) saplings exposed to low concentrations of ozone. , 1996, Tree physiology.

[57]  M. Petrini,et al.  Effects of air pollutants on the pathways of carbohydrate breakdown , 1994 .