Low-angle neutron scattering from chromatin subunit particles.

Monomer chromatin particles containing 140 base pairs of DNA and eight histone molecules have been studied by neutron scattering. From measurements in various H2O/D2O mixtures, radii of gyration and the average scattering density of the particle were determined. The radius of gyration under conditions when scattering from the DNA dominates is 50A, and when scattering from the protein dominates, 30A. Consequently the core of the particle is largely occupied by the histones while the outer shell consists of DNA together with some of the histone.

[1]  F. Young.  Biochemistry , 1955, The Indian Medical Gazette.

[2]  Masao Kakudo,et al.  Small Angle Scattering of X-Rays , 1968 .

[3]  G. E. BACON,et al.  Neutron Diffraction , 1972, Nature.

[4]  K. V. van Holde,et al.  Chromatographic separation of chromatin subunits. , 1974, Biochemical and biophysical research communications.

[5]  良二 上田.  J. Appl. Cryst.の発刊に際して , 1970 .