Utilization of photoinduced charge-separated state of donor-acceptor-linked molecules for regulation of cell membrane potential and ion transport.

The control of ion transport across cell membranes by light is an attractive strategy that allows targeted, fast control of precisely defined events in the biological membrane. Here we report a novel general strategy for the control of membrane potential and ion transport by using charge-separation molecules and light. Delivery of charge-separation molecules to the plasma membrane of PC12 cells by a membranous nanocarrier and subsequent light irradiation led to depolarization of the membrane potential as well as inhibition of the potassium ion flow across the membrane. Photoregulation of the cell membrane potential and ion transport by using charge-separation molecules is highly promising for control of cell functions.

[1]  I. Yamazaki,et al.  Substituent effects of porphyrin monolayers on the structure and photoelectrochemical properties of self-assembled monolayers of porphyrin on indium-tin oxide electrode , 2004 .

[2]  A. Fiala,et al.  Optogenetic Approaches in Neuroscience , 2010, Current Biology.

[3]  Cees Dekker,et al.  Motor Proteins at Work for Nanotechnology , 2007, Science.

[4]  E. Isacoff,et al.  Light-activated ion channels for remote control of neuronal firing , 2004, Nature Neuroscience.

[5]  Ehud Y Isacoff,et al.  Optical control of neuronal activity. , 2010, Annual review of biophysics.

[6]  Ying Yu,et al.  Anorexic effect of K+ channel blockade in mesenteric arterial smooth muscle and intestinal epithelial cells. , 2001, Journal of applied physiology.

[7]  Jochen Feldmann,et al.  Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding. , 2010, Nano letters.

[8]  Susana Q. Lima,et al.  Remote Control of Behavior through Genetically Targeted Photostimulation of Neurons , 2005, Cell.

[9]  Ben L Feringa,et al.  A Light-Actuated Nanovalve Derived from a Channel Protein , 2005, Science.

[10]  H. Pass,et al.  Photodynamic therapy in oncology: mechanisms and clinical use. , 1993, Journal of the National Cancer Institute.

[11]  L. Conforti,et al.  Selective inhibition of a slow‐inactivating voltage‐dependent K+ channel in rat PC 12 cells by hypoxia , 1997, The Journal of physiology.

[12]  M. Wasielewski,et al.  Electric Field Effects of Photogenerated Ion Pairs on Nearby Molecules: A Model for the Carotenoid Band Shift in Photosynthesis , 1995 .

[13]  I. Yamazaki,et al.  Vectorial Multistep Electron Transfer at the Gold Electrodes Modified with Self-Assembled Monolayers of Ferrocene−Porphyrin−Fullerene Triads , 2000 .

[14]  S. Fukuzumi,et al.  Modulating charge separation and charge recombination dynamics in porphyrin-fullerene linked dyads and triads: Marcus-normal versus inverted region. , 2001, Journal of the American Chemical Society.

[15]  E. Bamberg,et al.  Channelrhodopsin-1: A Light-Gated Proton Channel in Green Algae , 2002, Science.

[16]  Thomas A. Moore,et al.  Light-driven production of ATP catalysed by F0F1-ATP synthase in an artificial photosynthetic membrane , 1998, Nature.

[17]  P. Mullineaux,et al.  Imaging the production of singlet oxygen in vivo using a new fluorescent sensor, Singlet Oxygen Sensor Green. , 2006, Journal of experimental botany.

[18]  Sang Woong Park,et al.  Serotonin depolarizes the membrane potential in rat mesenteric artery myocytes by decreasing voltage-gated K+ currents. , 2006, Biochemical and biophysical research communications.

[19]  M. Hashida,et al.  Size control of lipid-based drug carrier by drug loading. , 2010, Molecular bioSystems.

[20]  Oded Shoseyov,et al.  Nanobiotechnology : bioinspired devices and materials of the future , 2008 .

[21]  M. Hashida,et al.  Intracellular drug delivery by genetically engineered high-density lipoprotein nanoparticles. , 2010, Nanomedicine.

[22]  G. Stark,et al.  Functional Consequences of Oxidative Membrane Damage , 2005, The Journal of Membrane Biology.