G-banding patterns of the chromosomes of amphioxus Branchiostoma belcheri tsingtauense.

Chromosome banding study remains lacking in amphioxus heretofore. This paper reports the G-band patterns of the metaphase chromosomes of amphioxus Branchiostoma belcheri tsingtauense, the first description and numbering of the karyotyped bands in a cephalochordate. G-banding results show that four out of the ten second pair chromosomes examined are heteromorphic, while the remaining ones (6/10) are homomorphic. In contrast, the other 17 pair chromosomes are all homomorphic. These broadly support our previous suggestion that the chromosomes No. 2 are a pair of sex-chromosomes. G-banding method stains positively 65.5% of the chromosome surface, and a total of 149 G-bands (77 positive, 65 negative and 7 variable) has been recorded in the 17 autosomes and the dimorphic chromosomes 2A' and 2A.

[1]  P. Holland,et al.  Fluorescent in situ Hybridisation to Amphioxus Chromosomes , 2002, Zoological science.

[2]  P. C. Blaxhall Chromosome karyotyping of fish using conventional and G‐banding methods , 1983 .

[3]  H. Boschung,et al.  Chromosomes of the lancelet,Branchiostoma floridae (order amphioxi) , 1971, Experientia.

[4]  M. Schmid Chromosome banding in amphibia , 2002, Cytogenetic and Genome Research.

[5]  M. Seabright A rapid banding technique for human chromosomes. , 1971, Lancet.

[6]  M. Rodrigues,et al.  Chromosomal banding patterns in the eyelid-less microteiid radiation: Procellosaurinus and Vanzosaura (Squamata, Gymnophthalmidae). , 1996, Cytogenetics and cell genetics.

[7]  Iscn International System for Human Cytogenetic Nomenclature , 1978 .

[8]  Chromosome banding in Amphibia , 2002, Cytogenetic and Genome Research.

[9]  M. Schmid,et al.  Evolutionary diversity of reverse (R) fluorescent chromosome bands in vertebrates , 2004, Chromosoma.

[10]  Zhang Shi Amphioxus-model animal for insights into the origin and evolution of the vertebrates , 2001 .

[11]  K. Sekiya,et al.  Cytogenetics ofXenopus laevis. I. G-banding pattern ofXenopus laevis chromosomes , 1983, Experientia.

[12]  B. Czepulkowski,et al.  Human cytogenetics : a practical approach , 1986 .

[13]  H. Wada,et al.  Details of the evolutionary history from invertebrates to vertebrates, as deduced from the sequences of 18S rDNA. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[14]  D. Colombera Male chromosomes in two populations ofBranchiostoma lanceolatum , 1974, Experientia.

[15]  Temporal and Spatial Profiles of Alkaline Phosphatase Activity During Embryogenesis of Amphioxus Branchiostoma belcheri tsingtaunese , 2001 .

[16]  P. Holland,et al.  Hox genes and chordate evolution. , 1996, Developmental biology.

[17]  A. Amores,et al.  Banded chromosomes and the zebrafish karyotype. , 1999, Methods in cell biology.

[18]  R. Drouin,et al.  High-resolution R-banding at the 1250-band level. III. Comparative analysis of morphologic and dynamic R-band patterns (RHG and RBG). , 2008, Hereditas.