Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody

summary A solid phase radioimmunoassay (RIA) has been successfully developed to measure both African swine fever virus (ASFV) antigen and antibody. Studies show that the assay is reproducible and will detect limiting antigen concentrations equivalent to 50–500 HAD50/ml. Both direct and indirect antibody RIA have been developed and have proved to be approximately 100 times more sensitive than the complement fixation test at present available and 1000 times more sensitive than the immuno-electro-osmophoresis test for the detection of ASFV antibody.

[1]  J. Docherty,et al.  Detection of antibodies specific for herpes simplex virus in human sera by the enzyme-linked immunosorbent assay. , 1977, The Journal of infectious diseases.

[2]  S. Baron,et al.  Successful prophylaxis against rabies in mice and Rhesus monkeys: the interferon system and vaccine. , 1977, The Journal of infectious diseases.

[3]  A. Ordas,et al.  African swine fever: comparison of four serotests on porcine serums in Spain. , 1974, American Journal of Veterinary Research.

[4]  A. Notkins,et al.  Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and antiviral antibody. , 1973, Applied microbiology.

[5]  Hess Wr,et al.  African swine fever: application of immunoelectroosmophoresis for the detection of antibody. , 1972 .

[6]  C. Williams.  Methods in Immunology and Immunochemistry , 1967 .

[7]  S. Stone,et al.  Fluorescent antibody studies on African swine fever virus. , 1966, American journal of veterinary research.

[8]  Stone Ss,et al.  PROPAGATION AND MODIFICATION OF AFRICAN SWINE FEVER VIRUS IN CELL CULTURES. , 1965 .

[9]  S. Stone,et al.  PROPAGATION AND MODIFICATION OF AFRICAN SWINE FEVER VIRUS IN CELL CULTURES. , 1965, American journal of veterinary research.

[10]  A. Sugiura,et al.  Procomplementary activity of swine serum and its elimination with periodate treatment. , 1962, Japanese journal of medical science & biology.

[11]  W. Malmquist,et al.  Hemadsorption and cytopathic effect produced by African Swine Fever virus in swine bone marrow and buffy coat cultures. , 1960, American journal of veterinary research.

[12]  R. E. Montgomery.  On A Form of Swine Fever Occurring in British East Africa (Kenya Colony) , 1921 .