Study on the expression and mechanism of plasma microRNA-21 in patients with ischemic cardiomyopathy.

OBJECTIVE To investigate the expression and mechanism of plasma microRNA-21 in patients with ischemic cardiomyopathy (ICM). PATIENTS AND METHODS 56 cases of ICM patients were selected in our hospital from February 2010 to March 2016 as the observation group, and 60 cases of healthy patients were selected as control group. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of microRNA-21 in two groups. Then, differences of the total cholesterol (TC), triglycerides (TG), left ventricular ejection fraction (LVEF), high and low density lipoprotein cholesterol (HDL-C, LDL-C), left ventricular end-diastolic volume (LVEDV), N terminal B type brain natriuretic peptide (NT-proBNP) and other clinical indicators of the two groups, were compared. The correlation between the plasma microRNA-21 level and the clinical indices was analyzed, and the value of microRNA-21 in the diagnosis and treatment of ICM was evaluated. RESULTS The levels of LDL-C, HDL-C and LVEF in the observation group were lower than those in the control group (p<0.05). Plasma microRNA-21, TG, NT-proBNP and LVEDV were higher than those in the control group; the difference was statistically significant (p<0.05). Logistic regression analysis showed that the plasma microRNA-21 level was positively correlated with NT-proBNP and LVEDV (p<0.05). CONCLUSIONS The expression of microRNA-21 in plasma of patients with ICM was significantly increased. And the expression of micro RNA-21 in plasma was positively correlated with NT-proBNP and LVEDV. Through the ventricular remodeling in ICM patients, it can be used as a new target for the diagnosis and treatment of ICM and a new biomarker for risk assessment.

[1]  A. Boening,et al.  Differential Expression of MicroRNAs in Endarterectomy Specimens Taken from Patients with Asymptomatic and Symptomatic Carotid Plaques , 2016, PloS one.

[2]  L. Nombela‐Franco,et al.  Ischemic Cardiomyopathy and Chronic Total Occlusions: Poor Prognosis, Worse in Combination. , 2016, JACC. Cardiovascular interventions.

[3]  V. Bollati,et al.  Titanium and Zirconium Levels Are Associated with Changes in MicroRNAs Expression: Results from a Human Cross-Sectional Study on Obese Population , 2016, PloS one.

[4]  Jui-Pin Wang,et al.  microRNA-21 mediates the TGF-β1-induced migration of keratinocytes via targeting PTEN. , 2016, European review for medical and pharmacological sciences.

[5]  D. Lai,et al.  Association between High Endocardial Unipolar Voltage and Improved Left Ventricular Function in Patients with Ischemic Cardiomyopathy. , 2016, Texas Heart Institute journal.

[6]  Shuangli Wang,et al.  Inhibition of microRNA-21 decreases the invasiveness of fibroblast-like synoviocytes in rheumatoid arthritis via TGFβ/Smads signaling pathway , 2016, Iranian journal of basic medical sciences.

[7]  Bing Liu,et al.  Identification of nondiabetic heart failure-associated genes by bioinformatics approaches in patients with dilated ischemic cardiomyopathy. , 2016, Experimental and therapeutic medicine.

[8]  Qiang-sun Zheng,et al.  Correlating blood levels of 8‐hydroxydeoxyguanosine to hOGG1 genotypes and the incidence of ischemic cardiomyopathy , 2016, The Kaohsiung journal of medical sciences.

[9]  X. Li,et al.  Deep RNA sequencing elucidates microRNA-regulated molecular pathways in ischemic cardiomyopathy and nonischemic cardiomyopathy. , 2016, Genetics and molecular research : GMR.

[10]  M. Clauss,et al.  Increased cardiovascular disease risk in the HIV-positive population on ART: potential role of HIV-Nef and Tat. , 2015, Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology.

[11]  Luke H. Hoeppner,et al.  Cardiomyopathy and Worsened Ischemic Heart Failure in SM22-&agr; Cre-Mediated Neuropilin-1 Null Mice: Dysregulation of PGC1&agr; and Mitochondrial Homeostasis , 2015, Arteriosclerosis, thrombosis, and vascular biology.

[12]  S. Griffiths-Jones,et al.  Fast-evolving microRNAs are highly expressed in the early embryo of Drosophila virilis , 2014, RNA.

[13]  U. Frey,et al.  Decreased Expression of miR-133a but Not of miR-1 is Associated with Signs of Heart Failure in Patients Undergoing Coronary Bypass Surgery , 2013, Cardiology.

[14]  Stewart T. Chang,et al.  Next-Generation Sequencing of Small RNAs from HIV-Infected Cells Identifies Phased microRNA Expression Patterns and Candidate Novel microRNAs Differentially Expressed upon Infection , 2013, mBio.

[15]  A. Morguet,et al.  Long-Term Prognostic Value of Restitution Slope in Patients with Ischemic and Dilated Cardiomyopathies , 2013, PloS one.

[16]  John O. Prior,et al.  Blood flow, flow reserve, and glucose utilization in viable and nonviable myocardium in patients with ischemic cardiomyopathy , 2013, European Journal of Nuclear Medicine and Molecular Imaging.

[17]  M. Rudin,et al.  Loss of AngiomiR-126 and 130a in Angiogenic Early Outgrowth Cells From Patients With Chronic Heart Failure: Role for Impaired In Vivo Neovascularization and Cardiac Repair Capacity , 2012, Circulation.

[18]  Yan-jun Qin,et al.  MicroRNA 21 Inhibits Left Ventricular Remodeling in the Early Phase of Rat Model with Ischemia-reperfusion Injury by Suppressing Cell Apoptosis , 2012, International journal of medical sciences.

[19]  Danish Sayed,et al.  MicroRNA-21 Is a Downstream Effector of AKT That Mediates Its Antiapoptotic Effects via Suppression of Fas Ligand* , 2010, The Journal of Biological Chemistry.

[20]  Chunxiang Zhang,et al.  MicroRNA Expression Signature and the Role of MicroRNA-21 in the Early Phase of Acute Myocardial Infarction* , 2009, The Journal of Biological Chemistry.

[21]  R. McKelvie,et al.  Canadian Cardiovascular Society Consensus Conference guidelines on heart failure, update 2009: diagnosis and management of right-sided heart failure, myocarditis, device therapy and recent important clinical trials. , 2009, The Canadian journal of cardiology.

[22]  Jeffrey E. Thatcher,et al.  Dysregulation of microRNAs after myocardial infarction reveals a role of miR-29 in cardiac fibrosis , 2008, Proceedings of the National Academy of Sciences.