The antioxidant activity and hypolipidemic activity of the total flavonoids from the fruit of Rosa laevigata Michx.

In the present work, the antioxidant activity in vitro and hypolipidemic activity of the total fla-vonoids (TFs) from the Rosa laevigata Michx fruit was evaluated, and the antioxidant effect in vivo was also discussed. The TFs exhibited a high scavenging effect on 2, 2-diphenyl-1- picrylhydrazyl (?DPPH) with IC50 values 0.01 mg/mL, and a stong reduce power in the test. Hyperli-pemic mice were intragastric administrated with TFs (25, 50 mg/kg/day) for 4 weeks, and fenofi-brate was used as the positive reference sub-stance. After the experiment, the levels of TC (total cholesterol), TG (triglyceride), LDL- C (low density lipoprotein-cholesterol) of the mice ad-ministrated with high-dose of TFs were mark-edly declined by 45.02%, 33.86% and 73.68%, re-spectively, while HDL-C (high density lipopro-tein-cholesterol) was significantly increased com-pared with model group. To investigate the hepatoprotective effect, histopathological assay, ALT (alanine aminotransferase), AST (aspartate aminotransefrase) and ALP (alkaline phosphatase) were also studied, and the results showed that TFs exhibited a favorable effect on liver protec-tion, of which the levels of ALT, AST and ALP were elevated by 55.85%, 29.15% and 25.68%, respectively. Furthermore, the TFs could sig-nificantly decrease the MDA (malondialdehyde) level and improve the levels of CAT (Catalas), SOD (superoxide dismutase), GSH (reduced glutathione), and GPX (glutathione peroxidase) compared with hyperlipemia mice. Our results suggested that TFs has a high antioxidant ac-tivity and hypolipidemic activity, which can be used as a potential medicine for cardiovascular diseases.

[1]  P. Diouf,et al.  Study on chemical composition, antioxidant and anti-inflammatory activities of hot water extract from Picea mariana bark and its proanthocyanidin-rich fractions , 2009 .

[2]  Chen Guang,et al.  Effect of the Lycium barbarum polysaccharides administration on blood lipid metabolism and oxidative stress of mice fed high-fat diet in vivo , 2009 .

[3]  G. Tenore,et al.  Saponins in Ipomoea batatas tubers: Isolation, characterization, quantification and antioxidant properties , 2009 .

[4]  Cheng Huang,et al.  Preventive effects of total flavonoids of Litsea coreana leve on hepatic steatosis in rats fed with high fat diet. , 2009, Journal of ethnopharmacology.

[5]  Bhavna Sharma,et al.  Hypoglycemic and hypolipidemic effects of flavonoid rich extract from Eugenia jambolana seeds on streptozotocin induced diabetic rats. , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[6]  M. S. Yang,et al.  Benzo[a]pyrene-induced elevation of GSH level protects against oxidative stress and enhances xenobiotic detoxification in human HepG2 cells. , 2007, Toxicology.

[7]  D. Sakthisekaran,et al.  Therapeutic effect of paclitaxel and propolis on lipid peroxidation and antioxidant system in 7,12 dimethyl benz(a)anthracene-induced breast cancer in female Sprague Dawley rats. , 2006, Life sciences.

[8]  Shu-Yao Tsai,et al.  Antioxidant properties of hot water extracts from Agrocybe cylindracea , 2006 .

[9]  K. Ng,et al.  Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica , 2006 .

[10]  Yikai Zhou,et al.  Fatty acid-mediated intracellular iron translocation: a synergistic mechanism of oxidative injury. , 2005, Free radical biology & medicine.

[11]  Jinyong Peng,et al.  Preparative isolation and separation of a novel and two known flavonoids from Patrinia villosa Juss by high-speed counter-current chromatography. , 2005, Journal of chromatography. A.

[12]  Xiao-ru Wang,et al.  The application of macroporous resins in the separation of licorice flavonoids and glycyrrhizic acid. , 2005, Journal of chromatography. A.

[13]  C. Lieber,et al.  Depressed hepatic glutathione and increased diene conjugates in alcoholic liver disease , 1983, Digestive Diseases and Sciences.

[14]  F A Kummerow,et al.  Cholesterol-rich diets have different effects on lipid peroxidation, cholesterol oxides, and antioxidant enzymes in rats and rabbits. , 2000, The Journal of nutritional biochemistry.

[15]  I. Fridovich.  Fundamental Aspects of Reactive Oxygen Species, or What's the Matter with Oxygen? , 1999, Annals of the New York Academy of Sciences.

[16]  B. Rao,et al.  Antidiabetic and hypolipidemic effects of Momordica cymbalaria Hook. fruit powder in alloxan-diabetic rats. , 1999, Journal of ethnopharmacology.

[17]  W. Frishman,et al.  Biologic markers as predictors of cardiovascular disease. , 1998, The American journal of medicine.

[18]  J. Fruchart,et al.  High density lipoproteins and coronary heart disease. Future prospects in gene therapy. , 1998, Biochimie.

[19]  T. Dinis,et al.  Antioxidant activities of some extracts of Thymus zygis. , 1997, Free radical research.

[20]  C. Anuradha,et al.  Effect of oral methionine on blood lipid peroxidation and antioxidants in alloxan-induced diabetic rats. , 1993, The Journal of nutritional biochemistry.

[21]  A. Welton,et al.  Flavonoids and arachidonic acid metabolism. , 1988, Progress in clinical and biological research.

[22]  J. Anderson,et al.  Dietary fiber: hyperlipidemia, hypertension, and coronary heart disease. , 1986, The American journal of gastroenterology.

[23]  S. Orrenius,et al.  Role of thiols in protection against biological reactive intermediates. , 1986, Advances in experimental medicine and biology.

[24]  B Chance,et al.  Hydroperoxide metabolism in mammalian organs. , 1979, Physiological reviews.

[25]  R. Levy,et al.  Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. , 1972, Clinical chemistry.

[26]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.