Increased Formation of the Isoprostanes IPF2α-I and 8-Epi-Prostaglandin F2α in Acute Coronary Angioplasty

Background The role of oxidant stress in cardiac ischemia/reperfusion injury in humans remains controversial. This is due, in part, to the limitations of available indices of oxidant stress in vivo. Isoprostanes are stable, free radical–catalyzed products of arachidonic acid. We assessed their formation in patients undergoing coronary reperfusion via percutaneous transluminal coronary angioplasty (PTCA). Methods and Results We developed specific, mass spectrometry assays for two structurally distinct F2 isoprostanes, 8-epi-PGF2α and IPF2α-I. Urine samples for isoprostane determination were collected in patients undergoing coronary arteriography (n=11), elective PTCA (n=15), and angiography after thrombolysis for acute myocardial infarction (MI) (n=10). Urinary levels (pmol/mmol creatinine) of both isoprostanes were markedly increased from baseline in the first 6 hours after PTCA for acute MI (105±17.8 versus 230±66 for 8-epi-PGF2α [P=.009] and 466±91 versus 833±153 for IPF2α-I [P=.001]) and returned towar...

[1]  H. Esterbauer,et al.  Continuous Monitoring of in Vztro Oxidation of Human Low Density Lipoprotein , 2009 .

[2]  G. FitzGerald,et al.  Localization of distinct F2-isoprostanes in human atherosclerotic lesions. , 1997, The Journal of clinical investigation.

[3]  D. Praticò,et al.  8-Epi PGF2α Generation During Coronary Reperfusion , 1997 .

[4]  F. Hsu,et al.  Mass Spectrometric Quantification of Markers for Protein Oxidation by Tyrosyl Radical, Copper, and Hydroxyl Radical in Low Density Lipoprotein Isolated from Human Atherosclerotic Plaques* , 1997, The Journal of Biological Chemistry.

[5]  G. FitzGerald,et al.  Total synthesis of a novel isoprostane IPF2α-I and its identification in biological fluids , 1996 .

[6]  M. Reilly,et al.  Modulation of oxidant stress in vivo in chronic cigarette smokers. , 1996, Circulation.

[7]  G. FitzGerald,et al.  Local Amplification of Platelet Function by 8-Epi Prostaglandin F2α Is Not Mediated by Thromboxane Receptor Isoforms* , 1996, The Journal of Biological Chemistry.

[8]  J. Manson,et al.  Lack of effect of long-term supplementation with beta carotene on the incidence of malignant neoplasms and cardiovascular disease. , 1996, The New England journal of medicine.

[9]  R. J. Waugh,et al.  Mass spectrometric analysis of four regioisomers of F2-isoprostanes formed by free radical oxidation of arachidonic acid , 1996, Journal of the American Society for Mass Spectrometry.

[10]  G. FitzGerald,et al.  Generation of 8-epiprostaglandin F2alpha by human monocytes. Discriminate production by reactive oxygen species and prostaglandin endoperoxide synthase-2. , 1996, The Journal of biological chemistry.

[11]  F. Kelly,et al.  Randomised controlled trial of vitamin E in patients with coronary disease: Cambridge Heart Antioxidant Study (CHAOS) , 1996, The Lancet.

[12]  J. Morrow,et al.  The isoprostanes. Current knowledge and directions for future research. , 1996, Biochemical pharmacology.

[13]  W. H. Betts,et al.  N-acetylcysteine in combination with nitroglycerin and streptokinase for the treatment of evolving acute myocardial infarction. Safety and biochemical effects. , 1995, Circulation.

[14]  G. FitzGerald,et al.  Immunological characterization of urinary 8-epi-prostaglandin F2 alpha excretion in man. , 1995, The Journal of pharmacology and experimental therapeutics.

[15]  R. Murphy,et al.  Isoleukotrienes Are Biologically Active Free Radical Products of Lipid Peroxidation (*) , 1995, The Journal of Biological Chemistry.

[16]  J. Morrow,et al.  Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. Smoking as a cause of oxidative damage. , 1995, The New England journal of medicine.

[17]  G. FitzGerald,et al.  Cylooxygenase-dependent Formation of the Isoprostane, 8-Epi Prostaglandin F2α(*) , 1995, The Journal of Biological Chemistry.

[18]  G. FitzGerald,et al.  Cyclooxygenase‐Dependent Formation of the Isoprostane 8‐EPI Prostaglandin F2α a , 1994, The Journal of biological chemistry.

[19]  B. Halliwell,et al.  Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? , 1994, The Lancet.

[20]  L. Tavazzi,et al.  Spontaneous Delayed Recovery of Perfusion and Contraction After the First 5 Weeks After Anterior Infarction: Evidence for the Presence of Hibernating Myocardium in the Infarcted Area , 1994, Circulation.

[21]  P. Halushka,et al.  Antiaggregatory activity of 8-epi-prostaglandin F2 alpha and other F-series prostanoids and their binding to thromboxane A2/prostaglandin H2 receptors in human platelets. , 1994, The Journal of pharmacology and experimental therapeutics.

[22]  A. Astrup,et al.  Oxidative DNA damage correlates with oxygen consumption in humans , 1994, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[23]  E. Topol,et al.  Recombinant human superoxide dismutase (h-SOD) fails to improve recovery of ventricular function in patients undergoing coronary angioplasty for acute myocardial infarction. , 1994, Circulation.

[24]  R. Peto,et al.  Antioxidant vitamins--benefits not yet proved. , 1994, The New England journal of medicine.

[25]  J. Morrow,et al.  Free radical-induced generation of isoprostanes in vivo. Evidence for the formation of D-ring and E-ring isoprostanes. , 1994, The Journal of biological chemistry.

[26]  J. Thornby,et al.  Direct evidence that the hydroxyl radical plays a pathogenetic role in myocardial "stunning" in the conscious dog and demonstration that stunning can be markedly attenuated without subsequent adverse effects. , 1993, Circulation research.

[27]  R. Bolli,et al.  Demonstration of free radical generation in the "stunned" myocardium in the conscious dog and identification of major differences between conscious and open-chest dogs. , 1993, The Journal of clinical investigation.

[28]  J. Morrow,et al.  Evidence for the existence of F2-isoprostane receptors on rat vascular smooth muscle cells. , 1993, The American journal of physiology.

[29]  B. Halliwell,et al.  Lipid peroxidation: its mechanism, measurement, and significance. , 1993, The American journal of clinical nutrition.

[30]  D. Ramsdale,et al.  Free-radical generation during angioplasty reperfusion for acute myocardial infarction , 1993, The Lancet.

[31]  V. Mišík,et al.  Detection of alkoxyl and carbon-centered free radicals in coronary sinus blood from patients undergoing elective cardioplegia. , 1993, Free radical biology & medicine.

[32]  J. Morrow,et al.  Formation of novel non-cyclooxygenase-derived prostanoids (F2-isoprostanes) in carbon tetrachloride hepatotoxicity. An animal model of lipid peroxidation. , 1992, The Journal of clinical investigation.

[33]  R. Bolli.  Myocardial 'stunning' in man. , 1992, Circulation.

[34]  J. Morrow,et al.  Non-cyclooxygenase-derived prostanoids (F2-isoprostanes) are formed in situ on phospholipids. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[35]  J. Morrow,et al.  Glomerular actions of a free radical-generated novel prostaglandin, 8-epi-prostaglandin F2 alpha, in the rat. Evidence for interaction with thromboxane A2 receptors. , 1992, The Journal of clinical investigation.

[36]  J. Anderson,et al.  Effects of early thrombolytic therapy (anistreplase versus streptokinase) on enzymatic and electrocardiographic infarct size in acute myocardial infarction. TEAM-2 Investigators. , 1991, The American journal of cardiology.

[37]  C. Wolfe,et al.  Acute myocardial dysfunction and recovery: a common occurrence after coronary bypass surgery. , 1990, Journal of the American College of Cardiology.

[38]  S. Davies,et al.  Lipid peroxidation associated with successful thrombolysis , 1990, The Lancet.

[39]  G. FitzGerald,et al.  Platelet and vascular function during coronary thrombolysis with tissue-type plasminogen activator. , 1989, Circulation.

[40]  H. Gruchow,et al.  The influence of smoking on vitamin C status in adults. , 1989, American journal of public health.

[41]  G. FitzGerald,et al.  Estimated rate of thromboxane secretion into the circulation of normal humans. , 1986, The Journal of clinical investigation.

[42]  G. FitzGerald,et al.  Analysis of prostacyclin and thromboxane biosynthesis in cardiovascular disease. , 1983, Circulation.

[43]  D. Praticò,et al.  Increased oxidant stress in liver cirrhosis , 1996 .

[44]  T. Slater,et al.  Allopurinol pretreatment improves postoperative recovery and reduces lipid peroxidation in patients undergoing coronary artery bypass grafting. , 1994, The Journal of thoracic and cardiovascular surgery.

[45]  K. Cheeseman,et al.  Measuring free radical reactions in vivo. , 1993, British medical bulletin.

[46]  T. Slater,et al.  Detection of free radicals and cholesterol hydroperoxides in blood taken from the coronary sinus of man during percutaneous transluminal coronary angioplasty. , 1991, Free radical research communications.

[47]  O. Alfieri,et al.  Occurrence of oxidative stress during reperfusion of the human heart. , 1990, Circulation.

[48]  B. Ames,et al.  Assay of lipid hydroperoxides using high-performance liquid chromatography with isoluminal chemiluminescence detection. , 1990, Methods in enzymology.

[49]  J. Morrow,et al.  Noncyclooxygenase oxidative formation of a series of novel prostaglandins: analytical ramifications for measurement of eicosanoids. , 1990, Analytical biochemistry.

[50]  P. Campíns-Falcó,et al.  Kinetic and chemometric studies of the determination of creatinine using the Jaffé reaction. Part I. Kinetics of the reaction: analytical conclusions. , 1989, The Analyst.

[51]  A. V. Van rij,et al.  Plasma thiobarbituric acid reactivity: reaction conditions and the role of iron, antioxidants and lipid peroxy radicals on the quantitation of plasma lipid peroxides. , 1988, Life sciences.

[52]  G. FitzGerald,et al.  Marked platelet activation in vivo after intravenous streptokinase in patients with acute myocardial infarction. , 1988, Circulation.