Lab-on-CMOS capacitance sensor array for real-time cell viability measurements with I2C readout

Capacitance sensing is an emerging technology for monitoring cell viability. This work extends a previously developed sensor that measured capacitive loading by cells on the oscillation frequency of a current-starved ring oscillator and converted the frequency to a digital value by counting oscillation cycles. The new sensor array has been developed into a one-chip lab-on-CMOS system with integrated temperature sensors, serial readout to an external microcontroller using an Inter-Integrated Circuit (I2C) bus, and automatic scanning to allow for autonomous data collection. To allow sensing at the required aF levels, the system was realized on single chip to reduce the baseline capacitance, and long counting times were employed. The I2C module was moved to the edge of the chip prevent exposing cells to unacceptably high temperatures during viability studies.

[1]  Denis Flandre,et al.  A 16 $\times$ 16 CMOS Capacitive Biosensor Array Towards Detection of Single Bacterial Cell , 2016, IEEE Transactions on Biomedical Circuits and Systems.

[2]  Pamela Abshire,et al.  High resolution capacitance sensor array for real-time monitoring of cell viability , 2014, 2014 IEEE International Symposium on Circuits and Systems (ISCAS).

[3]  Pamela Abshire,et al.  Tracking cancer cell proliferation on a CMOS capacitance sensor chip. , 2008, Biosensors & bioelectronics.

[4]  David Blaauw,et al.  An ultra low power 1V, 220nW temperature sensor for passive wireless applications , 2008, 2008 IEEE Custom Integrated Circuits Conference.

[5]  Elisabeth Smela,et al.  Multiple frequency dielectrophoresis , 2007, Electrophoresis.

[6]  P. Abshire,et al.  On-Chip Capacitance Sensing for Cell Monitoring Applications , 2007, IEEE Sensors Journal.

[7]  Nobuo Fujii,et al.  Tuning Analysis of a CMOS Current Controlled Ring Oscillator , 2006 .

[8]  Marco Crepaldi,et al.  A 130 nm Event-Driven Voltage and Temperature Insensitive Capacitive ROC , 2014, 2014 17th Euromicro Conference on Digital System Design.

[9]  Pamela Abshire,et al.  Packaging commercial CMOS chips for lab on a chip integration. , 2014, Lab on a chip.

[10]  A.A. Abidi,et al.  Phase Noise and Jitter in CMOS Ring Oscillators , 2006, IEEE Journal of Solid-State Circuits.

[11]  Pamela Abshire,et al.  A Fully Differential Rail-to-Rail CMOS Capacitance Sensor With Floating-Gate Trimming for Mismatch Compensation , 2009, IEEE Transactions on Circuits and Systems I: Regular Papers.

[12]  J. O'Dowd,et al.  Capacitive sensor interfacing using sigma-delta techniques , 2005, IEEE Sensors, 2005..

[13]  Eby G. Friedman,et al.  Sizing CMOS inverters with Miller Effect and Threshold voltage Variations , 2006, J. Circuits Syst. Comput..