Rapid determination of safranal in the quality control of saffron spice (Crocus sativus L.)

Abstract A method to determine safranal content based on non-polar solvent extraction followed by UV–Vis analysis available in the industry for quality control of saffron spice has been studied. Ultrasound-assisted extraction of safranal was carried out and optimised with respect to the solvent: diethyl ether, hexane and chloroform; the time of extraction; and the concentration of saffron in each organic solvent. Best extraction conditions were obtained when 20 g L −1 of saffron was extracted with chloroform for 15 min. Intra-laboratory validation of the optimised conditions and analysis by UV–Vis spectrophotometry showed satisfactory results in linearity, repeatability, intermediate precision and recovery. The limit of detection was 1 mg safranal kg −1 saffron and the limit of quantification was 3 mg safranal kg −1 saffron.

[1]  P. Tarantilis,et al.  Qualitative determination of volatile compounds and quantitative evaluation of safranal and 4-hydroxy-2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde (HTCC) in Greek saffron. , 2004, Journal of agricultural and food chemistry.

[2]  G. A. Ravishankar,et al.  Methods for the analysis of the saffron metabolites crocin, crocetins, picrocrocin and safranal for the determination of the quality of the spice using thin-layer chromatography, high-performance liquid chromatography and gas chromatography , 1992 .

[3]  Amaya Zalacain,et al.  Near-infrared spectroscopy in saffron quality control: determination of chemical composition and geographical origin. , 2005, Journal of agricultural and food chemistry.

[4]  C. Pappas,et al.  Geographical differentiation of saffron by GC–MS/FID and chemometrics , 2009 .

[5]  M Carmona,et al.  A New Approach to Saffron Aroma , 2007, Critical reviews in food science and nutrition.

[6]  P. Tarantilis,et al.  Determination of saffron (Crocus sativus L.) components in crude plant extract using high-performance liquid chromatography-UV-visible photodiode-array detection-mass spectrometry. , 1995, Journal of chromatography. A.

[7]  M. Manfait,et al.  FT-IR, FT-Raman spectroscopic study of carotenoids from saffron (Crocus sativus L.) and some derivatives , 1998 .

[8]  Gonzalo L. Alonso,et al.  Worldwide market screening of saffron volatile composition , 2009 .

[9]  M. Negbi.  Saffron: Crocus sativus L. , 2003 .

[10]  Mohammad Mahdavi,et al.  EVALUATION OF ISO METHOD IN SAFFRON QUALIFICATION , 2006 .

[11]  Amaya Zalacain,et al.  Rapid determination of crocetin esters and picrocrocin from saffron spice (Crocus sativus L.) using UV-visible spectrophotometry for quality control. , 2008, Journal of agricultural and food chemistry.

[12]  P. Tarantilis,et al.  Comparison of the volatile composition in thyme honeys from several origins in Greece. , 2007, Journal of agricultural and food chemistry.

[13]  M Carmona,et al.  Screening method for the detection of artificial colours in saffron using derivative UV-Vis spectrometry after precipitation of crocetin , 2005, Food additives and contaminants.

[14]  G. Alonso,et al.  Determination of Safranal from Saffron (Crocus sativus L.) by Thermal Desorption−Gas Chromatography , 1996 .

[15]  M. Manfait,et al.  Separation of picrocrocin, cis-trans-crocins and safranal of saffron using high-performance liquid chromatography with photodiode-array detection. , 1994, Journal of chromatography. A.

[16]  G. Alonso,et al.  Solid-phase extraction for picrocrocin determination in the quality control of saffron spice (Crocus sativus L.) , 2009 .

[17]  Moschos G. Polissiou,et al.  Isolation and Identification of the Aroma Components from Saffron (Crocus sativus) , 1997 .

[18]  J. Iborra,et al.  A non-destructive method to determine the safranal content of saffron (Crocus sativus L.) by supercritical carbon dioxide extraction combined with high-performance liquid chromatography and gas chromatography. , 2000, Journal of biochemical and biophysical methods.

[19]  Amaya Zalacain,et al.  Analysis of saffron volatile fraction by TD–GC–MS and e-nose , 2006 .

[20]  K. Sink,et al.  Development of an improved procedure for extraction and quantitation of safranal in stigmas of Crocus sativus L. using high performance liquid chromatography , 2000 .