Abstract A study on algae and bacteria population changes, as a function of time, was carried out in a pilot scale oxidation channel bioreactor using a carrousel system. Total Coliforms, Pseudomonas aeruginosa, and Streptococcus faecalis, the most common bacteria found in sewage, Scenedesmus obliquus and Chlorella vulgaris were the microalgae considered in this work. Physicochemical parameters such as COD, BOD, Chlorophyll a, nitrogen, and phosphorous compounds were studied and determined during the experiments. It was demonstrated that the level of wastewater contamination could be predicted based on the bacterial and algae composition. The relationships between the algae and bacteria population, and the variation of these microorganism populations as a measurement of the level of purification were established. The oxidation channel was able to remove a considerable amount of organic matter and pathogenic microorganisms in a relatively short time. The nitrification process could not be measured. The increase in the relative concentration of microalgae contributed toward improving the global efficiency of the system as well as reducing the pathogenic bacteria population.