Effect of methacholine challenge on cellular composition of sputum induction

BACKGROUND Induced sputum and methacholine inhalation challenge are routinely used for the assessment of airway inflammation and airway hyperresponsiveness, respectively. This study investigates whether a methacholine challenge performed one hour before sputum induction alters the cellular and biochemical constituents of sputum. METHODS Sixteen stable asthmatic patients with lung function within the normal range underwent two sputum inductions within one week. One hour before one of the sputum inductions a methacholine challenge was performed. RESULTS There were no significant differences in total cell count, macrophages, neutrophils, eosinophils, lymphocytes, epithelial cells, ECP, and albumin between the two challenges. The repeatability of cell counts was good for all cells, ECP and albumin, but poor for total cells. CONCLUSIONS In patients with stable asthma a methacholine challenge carried out one hour before sputum induction does not significantly alter the cellular and biochemical constituents of sputum.

[1]  V. Brusasco,et al.  Dissociation between airway inflammation and airway hyperresponsiveness in allergic asthma. , 1998, American journal of respiratory and critical care medicine.

[2]  A. Spanevello,et al.  Induced sputum to assess airway inflammation: a study of reproducibility , 1997, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[3]  J. Fahy,et al.  Safety of one method of sputum induction in asthmatic subjects. , 1997, American journal of respiratory and critical care medicine.

[4]  P. Sterk,et al.  Repeatability of cellular and soluble markers of inflammation in induced sputum from patients with asthma. , 1996, The European respiratory journal.

[5]  F. Hargreave,et al.  Indices of airway inflammation in induced sputum: reproducibility and validity of cell and fluid-phase measurements. , 1996, American journal of respiratory and critical care medicine.

[6]  H. Boushey,et al.  Cellular and biochemical analysis of induced sputum from asthmatic and from healthy subjects. , 1993, The American review of respiratory disease.

[7]  R. Lundgren,et al.  Inhaled methacholine does not influence the cellular composition of bronchoalveolar lavage fluid , 1993, Allergy.

[8]  S. Fitzgerald,et al.  National Asthma Education Program Expert Panel Report , 1992 .

[9]  J. Denburg,et al.  Use of induced sputum cell counts to investigate airway inflammation in asthma. , 1992, Thorax.

[10]  M. Chan-yeung,et al.  Differences in mediator release between allergic rhinitis and asthma. , 1991, The Journal of allergy and clinical immunology.

[11]  S. Holgate,et al.  Cellular events in the bronchi in mild asthma and after bronchial provocation. , 1989, The American review of respiratory disease.

[12]  G. Benchetrit,et al.  Effect of acute hypoxia on lung transvascular filtration in anaesthetized dogs. , 1983, Bulletin europeen de physiopathologie respiratoire.

[13]  N. Thomson,et al.  Bronchial responsiveness to histamine or methacholine in asthma: measurement and clinical significance. , 1981, The Journal of allergy and clinical immunology.