Expression of the FGFR1 gene in human breast‐carcinoma cells

Fibroblast growth factors (FGF) constitute a family of at least 9 members which act through high‐affinity tyrosine‐kinase receptors encoded by 4 distinct genes. In humans, the FGFRI gene is located in chromosomal region 8pl2. Its amplification and expression were examined in a panel of 110 breast carcinoma samples by Southern‐ and Northern‐blot analyses. FGFRI was amplified in 9% and overexpressed in about 15% of the tumors studied. In situ hybridization experiments were performed on tissue sections of normal breast and tumors with a high level of FGFRI expression. In both normal and tumoral tissues, FGFRI RNA was detected in the epithelial cells. Overexpression of FGFRI seems to be associated with small, well‐differentiated diploid tumors. © 1994 Wiley‐Liss, Inc.

[1]  D. Birnbaum,et al.  Optimization of immunohistochemical detection of ERBB2 in human breast cancer: Impact of fixation , 1994, The Journal of pathology.

[2]  D. Birnbaum,et al.  p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results. , 1994, British Journal of Cancer.

[3]  S. Leinster,et al.  Comparative expression of fibroblast growth factor mRNAs in benign and malignant breast disease. , 1994, British Journal of Cancer.

[4]  H. Friess,et al.  Aberrant expression of type I fibroblast growth factor receptor in human pancreatic adenocarcinomas. , 1993, Cancer research.

[5]  P. Sismondi,et al.  FGFRI and PLAT genes and DNA amplification at 8p 12 in breast and ovarian cancers , 1993, Genes, chromosomes & cancer.

[6]  P. Hall,et al.  Immunolocalization of fibroblast growth factor receptor 1 and its ligands in human tissues. , 1993, Laboratory investigation; a journal of technical methods and pathology.

[7]  W. Mckeehan,et al.  Exon switching and activation of stromal and embryonic fibroblast growth factor (FGF)-FGF receptor genes in prostate epithelial cells accompany stromal independence and malignancy , 1993, Molecular and cellular biology.

[8]  P. Hall,et al.  The fibroblast growth factor and receptor multigene families , 1993, The Journal of pathology.

[9]  P. Rudland,et al.  Immunocytochemical identification of basic fibroblast growth factor in the developing rat mammary gland: variations in location are dependent on glandular structure and differentiation. , 1993, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  K. Horgan,et al.  Fibroblast stimulation of breast cancer cell growth in a serum-free system. , 1993, British Journal of Cancer.

[11]  J. Partanen,et al.  Amplification of fgfr4 gene in human breast and gynecological cancers , 1993, International journal of cancer.

[12]  M. Herlyn,et al.  Inhibition of the fibroblast growth factor receptor 1 (FGFR-1) gene in human melanocytes and malignant melanomas leads to inhibition of proliferation and signs indicative of differentiation. , 1992, Oncogene.

[13]  J. Bonneterre,et al.  Basic fibroblast growth factor (bFGF): Mitogenic activity and binding sites in human breast cancer , 1992, The Journal of Steroid Biochemistry and Molecular Biology.

[14]  R. Coombes,et al.  Expression of basic fibroblast growth factor, FGFR1 and FGFR2 in normal and malignant human breast, and comparison with other normal tissues. , 1992, British Journal of Cancer.

[15]  D. Birnbaum,et al.  Fusion and amplification of two originally non‐syntenic chromosomal regions in a mammary carcinoma cell line , 1992, Genes, chromosomes & cancer.

[16]  A. Saxena,et al.  Increased expression of genes from growth factor signaling pathways in glioblastoma cell lines. , 1992, Oncogene.

[17]  J. Foekens,et al.  Stromal influences on breast cancer cell growth. , 1992, British Journal of Cancer.

[18]  R. Coombes,et al.  Localization of basic fibroblast growth factor and transforming growth factor beta 1 in the human mammary gland. , 1991, Cancer research.

[19]  D. Birnbaum,et al.  BEK and FLG, two receptors to members of the FGF family, are amplified in subsets of human breast cancers. , 1991, Oncogene.

[20]  S. Li,et al.  Expression of multiple species of basic fibroblast growth factor mRNA and protein in normal and tumor-derived mammary epithelial cells in culture. , 1991, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[21]  F. Kern,et al.  Growth factor receptors and the progression of breast cancer. , 1990, Seminars in cancer biology.

[22]  P. Rudland,et al.  Synthesis of basic fibroblast growth factor upon differentiation of rat mammary epithelial to myoepithelial‐like cells in culture , 1990, Journal of cellular physiology.

[23]  P. Rudland,et al.  Appearance of basic fibroblast growth factor receptors upon differentiation of rat mammary epithelial to myoepithelial‐like cells in culture , 1990, Journal of cellular physiology.

[24]  D. Birnbaum,et al.  Amplification of FGF-related genes in human tumors: possible involvement of HST in breast carcinomas. , 1989, Oncogene.

[25]  K. Takahashi,et al.  Growth stimulation of human breast epithelial cells by basic fibroblast growth factor in serum‐free medium , 1989, International journal of cancer.

[26]  M. Reed,et al.  Paracrine influence of human breast stromal fibroblasts on breast epithelial cells: Secretion of a polypeptide which stimulates reductive 17β‐oestradiol dehydrogenase activity , 1988, International Journal of Cancer.

[27]  D G Wilkinson,et al.  A molecular analysis of mouse development from 8 to 10 days post coitum detects changes only in embryonic globin expression. , 1987, Development.

[28]  William H. Hartmann,et al.  The World Health Organization Histological Typing of Breast Tumors—Second Edition , 1982 .

[29]  R. Coombes,et al.  The effect of endocrine therapy on fibroblast growth factor-like activity in nitrosomethylurea-induced rat mammary tumours. , 1993, European journal of cancer.

[30]  D. Birnbaum,et al.  Complexité de la famille des facteurs de croissance : la preuve par 9 , 1993 .

[31]  M. Vijver,et al.  Molecular genetic changes in human breast cancer. , 1993 .

[32]  D. Moscatelli,et al.  The FGF family of growth factors and oncogenes. , 1992, Advances in cancer research.

[33]  D. Yee,et al.  Regulation of human breast cancer by secreted growth factors. , 1989, Acta oncologica.