论文信息 - COMPARISONS OF TWO MEDIA PROPOSED FOR THE ISOLATION OF BACTERIA FROM THE RUMEN

COMPARISONS OF TWO MEDIA PROPOSED FOR THE ISOLATION OF BACTERIA FROM THE RUMEN

Strikingly different procedures have been used recently in attempting isolation of rumen bacteria. Hungate (1947) used an inorganic saltssugar-rumen fluid medium without preliminary enrichment and incubated in roll tubes. Procedures similar to those of Hungate were followed by Sijpesteijn (1951), Bryant and Burkey (1953), and Underkofler et al. (1953). Huhtanen et al. (1951) isolated rumen bacteria from enrichment cultures using buffered, poised dilution blanks and a highly organic medium in Brewer anaerobic plates. The medium contained no rumen fluid. Bryant and Burkey (1953), in comparing the dilution procedures, found the buffered, poised dilution blanks to yield higher viable counts than physiological saline dilution blanks. In addition they reported higher counts and larger colonies from Hungates's (1947) inorganic salts-ugarrumen fluid medium than from Gall's (1947) organic medium, but no experimental details of the comparisons were given. The present report concerns direct comparisons of Hungate-type and Gall-type media with respect to: (a) total counts, (b) cellulolytic counts, (c) morphological diversity of isolates, and (d) the ability of organisms isolated in one medium to grow in the other.

Paul H. Smith | K. W. King

[1] M. P. Bryant,et al. Factors necessary for the growth of Bacteroides succinogenes in the volatile acid fraction of rumen fluid. , 1954, Science.

[2] W. D. Kitts,et al. Digestion by Rumen Microorganisms, Hydrolytic Products of Cellulose and the Cellulolytic Enzymes , 1954 .

[3] W. D. Kitts,et al. Soluble cellulose derivatives as substrate for ruminal microorganisms. , 1953, Archives of biochemistry and biophysics.

[4] M. P. Bryant,et al. Cultural Methods and Some Characteristics of Some of the More Numerous Groups of Bacteria in the Bovine Rumen , 1953 .

[5] K. El-Shazly. Degradation of protein in the rumen of the sheep. I. Some volatile fatty acids, including branched-chain isomers, found in vivo. , 1952, Biochemical Journal.

[6] C. N. Huhtanen,et al. IMPROVED TECHNIQUES FOR ISOLATING AND PURIFYING RUMEN ORGANISMS , 1952, Journal of bacteriology.

[7] R. E. Hungate,et al. The anaerobic mesophilic cellulolytic bacteria. , 1950, Bacteriological reviews.

[8] C. N. Stark,et al. The Isolation and Preliminary Study of Some Physiological Characteristics of the Predominating Flora from the Rumen of Cattle and Sheep , 1947 .

[9] A. Sijpesteijn. On Ruminococcus flavefaciens, a cellulose-decomposing bacterium from the rumen of sheep and cattle. , 1951, Journal of general microbiology.