Binding of the Helicobacter pyloriVacuolating Cytotoxin to Target Cells

ABSTRACT The vacuolating cytotoxin of Helicobacter pylori, VacA, enters the cytoplasm of target cells and causes vacuolar degeneration by interfering with late stages of endocytosis. By using indirect immunofluorescence and flow cytometry, we have demonstrated that VacA binds to specific high-affinity cell surface receptors and that this interaction is necessary for cell intoxication.

[1]  DONALD C. Williams,et al.  Guidebook to protein toxins and their use in cell biology , 1998 .

[2]  P L Bellon,et al.  Three-dimensional reconstruction of metal replicas of the Helicobacter pylori vacuolating cytotoxin. , 1998, Journal of structural biology.

[3]  R. Rappuoli,et al.  Helicobacter pylori toxin VacA induces vacuole formation by acting in the cell cytosol , 1997, Molecular microbiology.

[4]  R. Rappuoli,et al.  Detoxification of the Helicobacter pylori cytotoxin , 1997, Infection and immunity.

[5]  T. Hirayama,et al.  Helicobacter pylori vacuolating cytotoxin binds to the 140-kDa protein in human gastric cancer cell lines, AZ-521 and AGS. , 1997, Biochemical and biophysical research communications.

[6]  T. Cover,et al.  Acid-induced Dissociation of VacA, the Helicobacter pylori Vacuolating Cytotoxin, Reveals Its Pattern of Assembly , 1997, The Journal of cell biology.

[7]  T. Cover,et al.  Binding and internalization of the Helicobacter pylori vacuolating cytotoxin by epithelial cells , 1996, Infection and immunity.

[8]  P. Rautenberg,et al.  Molecular mechanisms of action of bacterial exotoxins. , 1996, Zentralblatt fur Bakteriologie : international journal of medical microbiology.

[9]  R. Rappuoli,et al.  Oligomeric and subunit structure of the Helicobacter pylori vacuolating cytotoxin , 1996, The Journal of cell biology.

[10]  R. Rappuoli,et al.  Helicobacter pylori cytotoxin: importance of native conformation for induction of neutralizing antibodies , 1995, Infection and immunity.

[11]  R. Rappuoli,et al.  Low pH Activates the Vacuolating Toxin of Helicobacter pylori, Which Becomes Acid and Pepsin Resistant (*) , 1995, The Journal of Biological Chemistry.

[12]  Joel Moss,et al.  Bacterial toxins and virulence factors in disease , 1995 .

[13]  E. Mekada.  [Diphtheria toxin receptor]. , 1993, Nihon saikingaku zasshi. Japanese journal of bacteriology.

[14]  E. Papini,et al.  Bafilomycin A1 inhibits Helicobacter pylori‐induced vacuolization of HeLa cells , 1993, Molecular microbiology.

[15]  J. Alouf,et al.  Sourcebook of bacterial protein toxins , 1991 .