3D silicon neural probe with integrated optical fibers for optogenetic modulation.

Optogenetics is a powerful modality for neural modulation that can be useful for a wide array of biomedical studies. Penetrating microelectrode arrays provide a means of recording neural signals with high spatial resolution. It is highly desirable to integrate optics with neural probes to allow for functional study of neural tissue by optogenetics. In this paper, we report the development of a novel 3D neural probe coupled simply and robustly to optical fibers using a hollow parylene tube structure. The device shanks are hollow tubes with rigid silicon tips, allowing the insertion and encasement of optical fibers within the shanks. The position of the fiber tip can be precisely controlled relative to the electrodes on the shank by inherent design features. Preliminary in vivo rat studies indicate that these devices are capable of optogenetic modulation simultaneously with 3D neural signal recording.

[1]  Yu-Chong Tai,et al.  Underwater flexible shear-stress sensor skins , 2004, 17th IEEE International Conference on Micro Electro Mechanical Systems. Maastricht MEMS 2004 Technical Digest.

[2]  M. Sidor,et al.  Timing matters: using optogenetics to chronically manipulate neural circuitry and rhythms , 2014, Front. Behav. Neurosci..

[3]  Patrick Degenaar,et al.  Multi-site optical excitation using ChR2 and micro-LED array , 2010, Journal of neural engineering.

[4]  Wen Li,et al.  Opto-μECoG Array: A Hybrid Neural Interface With Transparent μECoG Electrode Array and Integrated LEDs for Optogenetics , 2013, IEEE Transactions on Biomedical Circuits and Systems.

[5]  Feng Zhang,et al.  Channelrhodopsin-2 and optical control of excitable cells , 2006, Nature Methods.

[6]  G. Buzsáki,et al.  An implantable neural probe with monolithically integrated dielectric waveguide and recording electrodes for optogenetics applications , 2013, Journal of neural engineering.

[7]  Jonathan Bradley,et al.  Spatially Selective Holographic Photoactivation and Functional Fluorescence Imaging in Freely Behaving Mice with a Fiberscope , 2014, Neuron.

[8]  Anita Mahadevan-Jansen,et al.  Application of infrared light for in vivo neural stimulation. , 2005, Journal of biomedical optics.

[9]  I. Cyrus.  Ian A , 2008 .

[10]  W. Marsden.  I and J , 2012 .

[11]  E. Bamberg,et al.  Optogenetic stimulation of the auditory pathway. , 2014, The Journal of clinical investigation.

[12]  Edward S Boyden,et al.  Three-dimensional multiwaveguide probe array for light delivery to distributed brain circuits. , 2012, Optics letters.

[13]  Nathan C. Klapoetke,et al.  A High-Light Sensitivity Optical Neural Silencer: Development and Application to Optogenetic Control of Non-Human Primate Cortex , 2010, Front. Syst. Neurosci..

[14]  Murtaza Z Mogri,et al.  Targeting and Readout Strategies for Fast Optical Neural Control In Vitro and In Vivo , 2007, The Journal of Neuroscience.

[15]  R. Stephenson.  A and V , 1962, The British journal of ophthalmology.

[16]  J. Assad,et al.  Multipoint-Emitting Optical Fibers for Spatially Addressable In Vivo Optogenetics , 2014, Neuron.

[17]  Weihua Pei,et al.  A fiber-based implantable multi-optrode array with contiguous optical and electrical sites , 2013, Journal of neural engineering.

[18]  Lief E. Fenno,et al.  Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins , 2011, Nature Methods.

[19]  Hung Cao,et al.  An Integrated μLED Optrode for Optogenetic Stimulation and Electrical Recording , 2013, IEEE Transactions on Biomedical Engineering.

[20]  I. Goshen.  The optogenetic revolution in memory research , 2014, Trends in Neurosciences.

[21]  K. Deisseroth,et al.  Millisecond-timescale, genetically targeted optical control of neural activity , 2005, Nature Neuroscience.

[22]  Michael A. Henninger,et al.  High-Performance Genetically Targetable Optical Neural Silencing via Light-Driven Proton Pumps , 2010 .

[23]  O. Paul,et al.  Ultracompact optrode with integrated laser diode chips and SU-8 waveguides for optogenetic applications , 2013, 2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS).

[24]  Feng Zhang,et al.  Multimodal fast optical interrogation of neural circuitry , 2007, Nature.

[25]  Hajime Hirase,et al.  A simple head-mountable LED device for chronic stimulation of optogenetic molecules in freely moving mice , 2011, Neuroscience Research.

[26]  Adam Huang,et al.  Flexible shear-stress sensor skin and its application to unmanned aerial vehicles , 2003 .

[27]  B. Connors,et al.  Integrated device for optical stimulation and spatiotemporal electrical recording of neural activity in light-sensitized brain tissue , 2009, Journal of neural engineering.

[28]  Wen Li,et al.  Integrated slanted microneedle-LED array for optogenetics , 2013, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC).

[29]  Jing Wang,et al.  A coaxial optrode as multifunction write-read probe for optogenetic studies in non-human primates , 2013, Journal of Neuroscience Methods.

[30]  Yong Xu,et al.  IC-integrated flexible shear-stress sensor skin , 2003 .

[31]  Jeffrey A. Loeb,et al.  A hybrid silicon-parylene neural probe with locally flexible regions , 2014 .

[32]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[33]  Tommaso Fellin,et al.  Optical dissection of brain circuits with patterned illumination through the phase modulation of light , 2015, Journal of Neuroscience Methods.

[34]  Karl Deisseroth,et al.  Optogenetic Activation of an Inhibitory Network Enhances Feedforward Functional Connectivity in Auditory Cortex , 2013, Neuron.

[35]  Lief E. Fenno,et al.  The Microbial Opsin Family of Optogenetic Tools , 2011, Cell.

[36]  Zach DeVito,et al.  Opt , 2017 .

[37]  Stephen A. Allsop,et al.  Optogenetic insights on the relationship between anxiety-related behaviors and social deficits , 2014, Front. Behav. Neurosci..

[38]  E. Bamberg,et al.  Channelrhodopsin-2, a directly light-gated cation-selective membrane channel , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Garret D Stuber,et al.  Construction of implantable optical fibers for long-term optogenetic manipulation of neural circuits , 2011, Nature Protocols.

[40]  Anita Mahadevan-Jansen,et al.  Pulsed laser versus electrical energy for peripheral nerve stimulation , 2007, Journal of Neuroscience Methods.

[41]  V. Sohal,et al.  Optogenetic approaches for investigating neural pathways implicated in schizophrenia and related disorders. , 2014, Human molecular genetics.

[42]  Susana Q. Lima,et al.  PINP: A New Method of Tagging Neuronal Populations for Identification during In Vivo Electrophysiological Recording , 2009, PloS one.

[43]  Feng Zhang,et al.  An optical neural interface: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology , 2007, Journal of neural engineering.

[44]  Karl Deisseroth,et al.  Optogenetics in Neural Systems , 2011, Neuron.