The development of skin immersion clearing method for increasing of laser exposure efficiency on subcutaneous objects

In this paper we have studied effect of a hyperosmotic optical clearing agent (OCA), such as polyethylene glycol, on the fluorescence intensity from a target located in subcutaneous area in the model experiments. As a fluorescence agent the nanocomposite including gold nanorods with hematophorphyrin was used. The remitted fluorescent signal traveling to the tissue surface was monitored over time as the tissue was treated with the OCA. The detected fluorescent signal increased as the scattering in tissue samples was substantially reduced. The study has shown how OCA can be used to improve the detected signal at localization of subcutaneous target tissue at the photothermal or photodynamic therapy. Immersion clearing of skin can be also useful for improvement of laser exposure efficiency due to the increasing of light penetration depth.

[1]  E Duco Jansen,et al.  Effect of optical tissue clearing on spatial resolution and sensitivity of bioluminescence imaging. , 2006, Journal of biomedical optics.

[2]  I. Meglinski,et al.  Amending of fluorescence sensor signal localization in human skin by matching of the refractive index. , 2004, Journal of biomedical optics.

[3]  P ? ? ? ? ? ? ? % ? ? ? ? , 1991 .

[4]  Enkeleda Dervishi,et al.  Nanophotothermolysis of multiple scattered cancer cells with carbon nanotubes guided by time-resolved infrared thermal imaging. , 2009, Journal of biomedical optics.

[5]  Gracie Vargas,et al.  Morphological Changes in Blood Vessels Produced by Hyperosmotic Agents and Measured by Optical Coherence Tomography¶ , 2003, Photochemistry and photobiology.

[6]  Duco Jansen,et al.  Use of glycerol as an optical clearing agent for enhancing photonic transference and detection of Salmonella typhimurium through porcine skin. , 2006, Journal of biomedical optics.

[7]  M Landthaler,et al.  Simulations on the selectivity of 5-aminolaevulinic acid-induced fluorescence in vivo. , 1998, Journal of photochemistry and photobiology. B, Biology.

[9]  Christoph Abels,et al.  Indocyanine green (ICG) and laser irradiation induce photooxidation , 2000, Archives of Dermatological Research.

[10]  Valery V. Tuchin,et al.  ALTERATIONS IN AUTOFLUORESCENCE SIGNAL FROM RAT SKIN EX VIVO UNDER OPTICAL IMMERSION CLEARING , 2010 .

[11]  W. Hall,et al.  Selective Photothermolysis : Precise Microsurgery by Selective Absorption of Pulsed Radiation , 2005 .

[12]  Jay D. Humphrey,et al.  Influence of glycerol on the mechanical reversibility and thermal damage susceptibility of collagenous tissues , 2006, IEEE Transactions on Biomedical Engineering.

[13]  T. Dougherty.  HEMATOPORPHYRIN AS A PHOTOSENSITIZER OF TUMORS * , 1983, Photochemistry and photobiology.

[14]  Hidetake Imasato,et al.  A combination of techniques to evaluate photodynamic efficiency of photosensitizers , 2008 .

[15]  Valery V. Tuchin,et al.  Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues , 2008 .

[16]  J. Stuart Nelson,et al.  Measuring the effects of topically applied skin optical clearing agents and modeling the effects and consequences for laser therapies , 2005, SPIE BiOS.

[17]  Valery V. Tuchin,et al.  Optical amplification of photothermal therapy with gold nanoparticles and nanoclusters , 2006 .

[18]  Abdul W. Basit,et al.  Concentration-Dependent Effects of Polyethylene Glycol 400 on Gastrointestinal Transit and Drug Absorption , 2003, Pharmaceutical Research.

[19]  Valery V. Tuchin,et al.  Optical Clearing of Tissues and Blood , 2005 .

[20]  Sol Kimel,et al.  Influence of wavelength on response to laser photothermolysis of blood vessels: Implications for port wine stain laser therapy , 2003, Lasers in surgery and medicine.

[21]  Qingming Luo,et al.  IMPROVE OPTICAL CLEARING OF SKIN IN VITRO WITH PROPYLENE GLYCOL AS A PENETRATION ENHANCER , 2009 .

[22]  Yonghong He,et al.  Enhanced sensitivity and spatial resolution for in vivo imaging with low-level light-emitting probes by use of biocompatible chemical agents. , 2003, Optics letters.

[23]  Fridrun Podczeck,et al.  Influence of Polyethylene Glycol 400 on the Gastrointestinal Absorption of Ranitidine , 2002, Pharmaceutical Research.

[24]  Valery V. Tuchin,et al.  Light propagation in tissues with controlled optical properties , 1996, European Conference on Biomedical Optics.

[25]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[26]  A J Welch,et al.  Use of osmotically active agents to alter optical properties of tissue: Effects on the detected fluorescence signal measured through skin , 2001, Lasers in surgery and medicine.

[27]  Michael D Morris,et al.  Transcutaneous fiber optic Raman spectroscopy of bone using annular illumination and a circular array of collection fibers. , 2006, Journal of biomedical optics.

[28]  Valery V Tuchin,et al.  A pilot study of ICG laser therapy of acne vulgaris: Photodynamic and photothermolysis treatment , 2003, Lasers in surgery and medicine.