Immunoassay Techniques Highlighting Biomarkers in Immunogenetic Diseases

Diagnosis of autoimmune diseases is crucial for the clinician and the patient alike. The immunoassay techniques most commonly used for this purpose are immunohistochemistry, ELISA, and Western blotting. For the detection of more specific biomarkers or the discovery of new ones for diagnostic purposes and as therapeutic targets, microarray techniques are increasingly used, for example, protein microarray, Luminex, and in recent years, surface plasmon resonance imaging. All of these technologies have undergone changes over time, making them easier to use. Similar technologies have been invented but responding to specific requirements for both diagnostic and research purposes. The goals are to study more analytes in the same sample, in a shorter time, and with increased accuracy. The reproducibility and reliability of the results are also a target pursued by manufacturers. In this chapter, we present these technologies and their utility in the diagnosis of immunogenetic diseases.

[1]  L. Sternberger,et al.  THE UNLABELED ANTIBODY ENZYME METHOD OF IMMUNOHISTOCHEMISTRY PREPARATION AND PROPERTIES OF SOLUBLE ANTIGEN-ANTIBODY COMPLEX (HORSERADISH PEROXIDASE-ANTIHORSERADISH PEROXIDASE) AND ITS USE IN IDENTIFICATION OF SPIROCHETES , 1970, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[2]  E. Engvall,et al.  ELISA (Enzyme‐Linked Immunosorbent Assay) , 2002 .

[3]  I. Salamunić,et al.  Comparative analysis of multiplex AtheNA Multi-Lyte ANA test system and conventional laboratory methods to detect autoantibodies , 2008 .

[4]  B. Mayer,et al.  Detection of protein-protein interactions by far-western blotting. , 2009, Methods in molecular biology.

[5]  A. Rasooly,et al.  ELISA-LOC: lab-on-a-chip for enzyme-linked immunodetection. , 2010, Lab on a chip.

[6]  G. Borm,et al.  Diagnostic value of MHC class I staining in idiopathic inflammatory myopathies. , 2004, Journal of neurology, neurosurgery, and psychiatry.

[7]  P. Lindström,et al.  IgG Autoantibody to Human Serum Albumin Studied by the ELISA‐Technique , 1978, Scandinavian journal of immunology.

[8]  O Ouchterlony,et al.  A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. , 1983, Journal of immunological methods.

[9]  M. Uppin,et al.  Major histocompatibility complex class I expression can be used as a diagnostic tool to differentiate idiopathic inflammatory myopathies from dystrophies. , 2008, Neurology India.

[10]  Polyacrylamide gel photopatterning enables automated protein immunoblotting in a two-dimensional microdevice. , 2010, Journal of the American Chemical Society.

[11]  Erik Avaniss-Aghajani,et al.  Clinical Value of Multiplexed Bead-Based Immunoassays for Detection of Autoantibodies to Nuclear Antigens , 2007, Clinical and Vaccine Immunology.

[12]  E. Hoffman,et al.  Activation of the endoplasmic reticulum stress response in autoimmune myositis: potential role in muscle fiber damage and dysfunction. , 2005, Arthritis and rheumatism.

[13]  J. Reveille,et al.  Role of Protein Phosphatase Magnesium‐Dependent 1A and Anti–Protein Phosphatase Magnesium‐Dependent 1A Autoantibodies in Ankylosing Spondylitis , 2014, Arthritis & rheumatology.

[14]  R. Corn,et al.  Fabrication of DNA microarrays with poly(L-glutamic acid) monolayers on gold substrates for SPR imaging measurements. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[15]  E. Sasse,et al.  Competitive enzyme-liked immunoassay with use of soluble enzyme/antibody immune complexes for labeling. I. Measurement of human choriogonadotropin. , 1976, Clinical chemistry.

[16]  C. Sarkar,et al.  Major histocompatibility complex class I and II detection as a diagnostic tool in idiopathic inflammatory myopathies. , 2009, Archives of pathology & laboratory medicine.

[17]  T. Livache,et al.  Effects of formamide on the thermal stability of DNA duplexes on biochips. , 2010, Analytical biochemistry.

[18]  M. Mann.  Can proteomics retire the western blot? , 2008, Journal of proteome research.

[19]  S. Luo,et al.  Antibody arrays in biomarker discovery. , 2015, Advances in clinical chemistry.

[20]  S. Kondo,et al.  Detection of antinuclear antibodies by use of an enzyme immunoassay with nuclear HEp-2 cell extract and recombinant antigens: comparison with immunofluorescence assay in 307 patients. , 2001, Clinical chemistry.

[21]  Zhiguo Zhou,et al.  Single-Multiplex Detection of Organ Injury Biomarkers using SPRi based Nano-Immunosensor , 2016, Scientific Reports.

[22]  B. Bosch,et al.  Specific serology for emerging human coronaviruses by protein microarray. , 2013, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[23]  K. Pritzker,et al.  Serum levels of novel noggin and sclerostin-immune complexes are elevated in ankylosing spondylitis , 2013, Annals of the rheumatic diseases.

[24]  R. Schasfoort,et al.  Biomolecular interaction monitoring of autoantibodies by scanning surface plasmon resonance microarray imaging. , 2007, Journal of the American Chemical Society.

[25]  Chris Grant,et al.  Optimization of immobilized bacterial disaccharides for surface plasmon resonance imaging measurements of antibody binding. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[26]  R. Barresi.  From proteins to genes: immunoanalysis in the diagnosis of muscular dystrophies , 2011, Skeletal Muscle.

[27]  J. González-Buitrago.  Multiplexed testing in the autoimmunity laboratory , 2006, Clinical chemistry and laboratory medicine.

[28]  Concepción González,et al.  Clinical evaluation of a microsphere bead-based flow cytometry assay for the simultaneous determination of anti-thyroid peroxidase and anti-thyroglobulin antibodies. , 2005, Clinical biochemistry.

[29]  M. Mascini,et al.  Surface plasmon resonance imaging for affinity-based biosensors. , 2010, Biosensors & bioelectronics.

[30]  E. Ionica,et al.  MUSCULAR DYSTROPHIES PROTEINS EVALUATION BY WESTERN BLOTT AND IMMUNOFLUORESCENCE , 2008 .

[31]  M. Fritzler,et al.  A clinical approach to autoantibody testing in systemic autoimmune rheumatic disorders. , 2007, Autoimmunity reviews.

[32]  George Notas,et al.  Comparison of a multiplex, bead-based fluorescent assay and immunofluorescence methods for the detection of ANA and ANCA autoantibodies in human serum. , 2006, Journal of immunological methods.

[33]  J. Gu,et al.  HLA-B27 polymorphism in patients with juvenile and adult-onset ankylosing spondylitis in Southern China. , 2010, Tissue antigens.

[34]  Xiahui Bi,et al.  Isoelectric focusing technology quantifies protein signaling in 25 cells , 2006, Proceedings of the National Academy of Sciences.

[35]  L. Bujanda,et al.  Surface plasmon resonance immunoassay for the detection of the TNFα biomarker in human serum. , 2014, Talanta.

[36]  B. Liedberg,et al.  Surface plasmon resonance for gas detection and biosensing , 1983 .

[37]  R. Corn,et al.  Microarray methods for protein biomarker detection. , 2008, The Analyst.

[38]  N. Bizzaro,et al.  Immunoassay of Anti-Thyroid Autoantibodies: High Analytical Variability in Second Generation Methods , 2002, Clinical chemistry and laboratory medicine.

[39]  A. Rasooly,et al.  Miniaturized 96-well ELISA chips for staphylococcal enterotoxin B detection using portable colorimetric detector , 2009, Analytical and bioanalytical chemistry.

[40]  T. Jaskowski,et al.  Screening for antinuclear antibodies by enzyme immunoassay. , 1996, American journal of clinical pathology.

[41]  Raymond E Biagini,et al.  Analytical performance of the AtheNA MultiLyte® ANA II assay in sera from lupus patients with multiple positive ANAs , 2007, Analytical and bioanalytical chemistry.

[42]  Robert G. Cooper,et al.  Polymyositis and Dermatomyositis , 2022 .

[43]  G. Opiteck,et al.  Biomarker discovery in biological fluids. , 2005, Methods.

[44]  Weiwei Kong,et al.  Luminex xMAP combined with Western blot improves HIV diagnostic sensitivity. , 2016, Journal of virological methods.

[45]  M. Manns,et al.  Liver autoimmune serology: a consensus statement from the committee for autoimmune serology of the International Autoimmune Hepatitis Group. , 2004, Journal of hepatology.

[46]  E. Engvall,et al.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. , 1971, Immunochemistry.

[47]  W. Robinson,et al.  Proteomic biomarkers for autoimmune disease , 2006, Proteomics.

[48]  R. Burgos-Vargas,et al.  In Vivo Peripheral Blood Proinflammatory T Cells in Patients with Ankylosing Spondylitis , 2012, The Journal of Rheumatology.

[49]  J. Marrack.  Nature of Antibodies , 1934, Nature.

[50]  R. Inman,et al.  Endoplasmic reticulum aminopeptidase 1 (ERAP1) exhibits functionally significant interaction with HLA-B27 and relates to subtype specificity in ankylosing spondylitis , 2012, Annals of the rheumatic diseases.

[51]  D. Mason,et al.  Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents. , 1978, Journal of clinical pathology.

[52]  G. B. Pierce,et al.  ENZYME-LABELED ANTIBODIES: PREPARATION AND APPLICATION FOR THE LOCALIZATION OF ANTIGENS , 1966, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[53]  I. Peene,et al.  Detection of specific antinuclear reactivities in patients with negative anti-nuclear antibody immunofluorescence screening tests. , 2002, Clinical chemistry.

[54]  A. Bhalla,et al.  Association of cytokine and matrix metalloproteinase profiles with disease activity and function in ankylosing spondylitis , 2012, Arthritis Research & Therapy.

[55]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[56]  Giovanni B. Frisoni,et al.  Consensus report of the working group on: 'Molecular and biochemical markers of Alzheimer's disease' , 1998 .

[57]  Anne-Marie Rouquette,et al.  Evaluation of the new multiplexed immunoassay, FIDIS, for simultaneous quantitative determination of antinuclear antibodies and comparison with conventional methods. , 2003, American journal of clinical pathology.

[58]  A. Uzdensky,et al.  PDT-induced epigenetic changes in the mouse cerebral cortex: a protein microarray study. , 2014, Biochimica et biophysica acta.

[59]  T. Livache,et al.  Polypyrrole-peptide microarray for biomolecular interaction analysis by SPR imaging. , 2009, Methods in molecular biology.

[60]  W. N. Burnette,et al.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. , 1981, Analytical biochemistry.

[61]  P. Schur,et al.  ANA screening: an old test with new recommendations , 2010, Annals of the rheumatic diseases.

[62]  Amy E Herr,et al.  Microfluidic polyacrylamide gel electrophoresis with in situ immunoblotting for native protein analysis. , 2009, Analytical chemistry.

[63]  R. Kennedy,et al.  Western blotting using capillary electrophoresis. , 2011, Analytical chemistry.

[64]  I. Nabiev,et al.  Quantum-dot-based suspension microarray for multiplex detection of lung cancer markers: preclinical validation and comparison with the Luminex xMAP® system , 2017, Scientific Reports.

[65]  H. Seitz,et al.  Up-to-Date Applications of Microarrays and Their Way to Commercialization , 2015, Microarrays.

[66]  Alastair W Wark,et al.  Fabricating RNA microarrays with RNA-DNA surface ligation chemistry. , 2005, Analytical chemistry.

[67]  D. Kemp,et al.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[68]  Marvin J Fritzler,et al.  Analysis of human sera that are polyreactive in an addressable laser bead immunoassay. , 2006, Clinical immunology.

[69]  R. Schasfoort,et al.  Mapping of citrullinated fibrinogen B-cell epitopes in rheumatoid arthritis by imaging surface plasmon resonance , 2010, Arthritis research & therapy.

[70]  C. D. dos Remedios,et al.  An Antibody-Based Leukocyte-Capture Microarray for the Diagnosis of Systemic Lupus Erythematosus , 2013, PloS one.

[71]  Peter B Luppa,et al.  Novel biosensor-based analytic device for the detection of anti-double-stranded DNA antibodies. , 2007, Clinical chemistry.

[72]  L. Anderson,et al.  Multiplex Western blotting system for the analysis of muscular dystrophy proteins. , 1999, The American journal of pathology.

[73]  G. Hughes,et al.  Anti-52 kDa Ro, anti-60 kDa Ro, and anti-La antibody profiles in neonatal lupus. , 2004, The Journal of rheumatology.

[74]  Emanuel F Petricoin,et al.  Protein microarrays: meeting analytical challenges for clinical applications. , 2003, Cancer cell.

[75]  Rebecca L Rich,et al.  Higher-throughput, label-free, real-time molecular interaction analysis. , 2007, Analytical biochemistry.

[76]  J. Ramos-Vara,et al.  Comparison of two polymer‐based immunohistochemical detection systems: ENVISION+™ and ImmPRESS™ , 2006, Journal of microscopy.

[77]  C. Francí,et al.  Further studies on the ELISA-spot technique. Its application to particulate antigens and a potential improvement in sensitivity. , 1986, Journal of immunological methods.

[78]  I. Illa,et al.  Diagnostic utility of cortactin antibodies in myasthenia gravis , 2018, Annals of the New York Academy of Sciences.

[79]  Martin Eisenacher,et al.  Autoimmune profiling with protein microarrays in clinical applications. , 2014, Biochimica et biophysica acta.

[80]  S M Hanash,et al.  Biomedical applications of two‐dimensional electrophoresis using immobilized pH gradients: Current status , 2000, Electrophoresis.

[81]  D. Peritt,et al.  A novel monoclonal antibody screening method using the Luminex-100 microsphere system. , 2002, Journal of immunological methods.

[82]  R. Jones,et al.  Immunological Properties of an Antibody Containing a Fluorescent Group.∗ , 1941 .

[83]  M. Neagu,et al.  Circulating biomarker panels for targeted therapy in brain tumors. , 2015, Future oncology.

[84]  M. Sticherling,et al.  Comparison of Different Test Systems for Simultaneous Autoantibody Detection in Connective Tissue Diseases , 2005, Annals of the New York Academy of Sciences.

[85]  Concepción González,et al.  Antinuclear antibodies (ANA) screening by enzyme immunoassay with nuclear HEp-2 cell extract and recombinant antigens: analytical and clinical evaluation. , 2002, Clinical biochemistry.

[86]  Peter Nilsson,et al.  Antigen arrays for profiling autoantibody repertoires. , 2016, Bioanalysis.

[87]  W. Robinson,et al.  Multiplexed autoantigen microarrays identify HLA as a key driver of anti-desmoglein and -non-desmoglein reactivities in pemphigus , 2016, Proceedings of the National Academy of Sciences.

[88]  S. Orton,et al.  Practical Evaluation of Methods for Detection and Specificity of Autoantibodies to Extractable Nuclear Antigens , 2004, Clinical Diagnostic Laboratory Immunology.

[89]  J. Sibilia,et al.  Heterogeneous clinical spectrum of anti-SRP myositis and importance of the methods of detection of anti-SRP autoantibodies: a multicentric study , 2016, Immunologic research.

[90]  R. Rizzo,et al.  The dimeric form of HLA-G molecule is associated with the response of early rheumatoid arthritis (ERA) patients to methotrexate , 2017, Clinical Rheumatology.

[91]  Jochen M. Schwenk,et al.  Current applications of antibody microarrays , 2018, Clinical Proteomics.

[92]  D. Kardassis,et al.  Anti-tumor necrosis factor therapy improves insulin resistance, beta cell function and insulin signaling in active rheumatoid arthritis patients with high insulin resistance , 2012, Arthritis Research & Therapy.

[93]  S. Fuggle,et al.  THE DETAILED DISTRIBUTION OF MHC CLASS II ANTIGENS IN NORMAL HUMAN ORGANS , 1984, Transplantation.

[94]  C. Tanase,et al.  Cytokine Patterns in Brain Tumour Progression , 2013, Mediators of inflammation.

[95]  M. Dalakas.  Inflammatory muscle diseases: a critical review on pathogenesis and therapies. , 2010, Current opinion in pharmacology.

[96]  B. van Weemen,et al.  Immunoassay using antigen—enzyme conjugates , 1971, FEBS letters.

[97]  Yordan Kostov,et al.  An ELISA Lab-on-a-Chip (ELISA-LOC). , 2013, Methods in molecular biology.

[98]  H. Mischak,et al.  Urinary peptidomics in kidney disease and drug research , 2018, Expert opinion on drug discovery.

[99]  A. Ray,et al.  Surface plasmon resonance imaging for biosensing. , 2009, IET nanobiotechnology.

[100]  J. P. McCoy,et al.  Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA. , 2006, Methods.

[101]  N. Szewczyk,et al.  An overview of technical considerations for Western blotting applications to physiological research , 2017, Scandinavian journal of medicine & science in sports.

[102]  R. Hohlfeld,et al.  The immunobiology of muscle. , 1994, Immunology today.

[103]  D. Figarella-Branger,et al.  Class I MHC detection as a diagnostic tool in noninformative muscle biopsies of patients suffering from dermatomyositis (DM) , 2003, Neuropathology and applied neurobiology.

[104]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[105]  Allan Wiik,et al.  Detection of antinuclear antibodies by solid-phase immunoassays and immunofluorescence analysis. , 2004, Clinical chemistry.

[106]  G. Hu,et al.  Parallel detection of autoantibodies with microarrays in rheumatoid diseases. , 2004, Clinical chemistry.

[107]  Shaohui Hu,et al.  Severe acute respiratory syndrome diagnostics using a coronavirus protein microarray. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[108]  R. H. Scofield,et al.  Western Blotting , 2015, Methods in Molecular Biology.

[109]  Ian Todd,et al.  ELISA in the multiplex era: Potentials and pitfalls , 2015, Proteomics. Clinical applications.

[110]  Michael Schlosser,et al.  Diabetes Antibody Standardization Program: evaluation of assays for autoantibodies to glutamic acid decarboxylase and islet antigen-2 , 2008, Diabetologia.

[111]  G. Pruijn,et al.  ACPA fine-specificity profiles in early rheumatoid arthritis patients do not correlate with clinical features at baseline or with disease progression , 2013, Arthritis Research & Therapy.

[112]  S. Bellon,et al.  Hyphenation of surface plasmon resonance imaging to matrix-assisted laser desorption ionization mass spectrometry by on-chip mass spectrometry and tandem mass spectrometry analysis. , 2009, Analytical chemistry.

[113]  T. Huizinga,et al.  Autoantibodies recognizing carbamylated proteins are present in sera of patients with rheumatoid arthritis and predict joint damage , 2011, Proceedings of the National Academy of Sciences.

[114]  Jennifer E. Gilda,et al.  The necessity of and strategies for improving confidence in the accuracy of western blots , 2014, Expert review of proteomics.

[115]  I. Auger,et al.  New autoantibodies in early rheumatoid arthritis , 2013, Arthritis Research & Therapy.

[116]  S. Hsu,et al.  Protein A, avidin, and biotin in immunohistochemistry. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[117]  M J Fritzler,et al.  Advances and Applications of Multiplexed Diagnostic Technologies in Autoimmune Diseases , 2006, Lupus.

[118]  Anders A. Bengtsson,et al.  Serum Protein Profiling of Systemic Lupus Erythematosus and Systemic Sclerosis Using Recombinant Antibody Microarrays* , 2011, Molecular & Cellular Proteomics.

[119]  Lawrence Steinman,et al.  Proteomics technologies for the study of autoimmune disease. , 2002, Arthritis and rheumatism.

[120]  J. Coorssen,et al.  Quantitative femto- to attomole immunodetection of regulated secretory vesicle proteins critical to exocytosis. , 2002, Analytical biochemistry.

[121]  V. Limaye,et al.  Major Histocompatibility Complex Class I and II Expression in Idiopathic Inflammatory Myopathy , 2013, Applied immunohistochemistry & molecular morphology : AIMM.

[122]  S. Haeghebaert,et al.  BULLETIN EUROPÉEN SUR LES MALADIES TRANSMISSIBLES / EUROPEAN COMMUNICABLE DISEASE BULLETIN , 2003 .