论文信息 - Existence of human DAZLA protein in the cytoplasm of human oocytes.

Existence of human DAZLA protein in the cytoplasm of human oocytes.

The human deleted in azoospermia-like autosomal (DAZLA) gene is thought to be a candidate gene for azoospermia. cDNA encoding the C-terminal 94 amino acid residues of human DAZLA was used to express a bacterial fusion protein which was then used to raise polyclonal antibodies in rabbits. Immunohistochemical analyses with the human DAZLA antiserum showed that the DAZLA protein is expressed at a cytoplasmic location in female germ cells. Available evidence suggests that the DAZLA gene is a participant in human oogenesis.

M. Kasahara | S. Fujimoto | T. Ishibashi | N. Hoshi | S. Nishi

[1] M. Ruggiu,et al. The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis , 1997, Nature.

[2] T. Bourgeron,et al. Gene sequence, localization, and evolutionary conservation of DAZLA, a candidate male sterility gene. , 1997, Genomics.

[3] L. Brown,et al. Mouse autosomal homolog of DAZ, a candidate male sterility gene in humans, is expressed in male germ cells before and after puberty. , 1996, Genomics.

[4] D. Page,et al. Severe oligozoospermia resulting from deletions of azoospermia factor gene on Y chromosome , 1996, The Lancet.

[5] M. Ruggiu,et al. A murine homologue of the human DAZ gene is autosomal and expressed only in male and female gonads. , 1996, Human molecular genetics.

[6] S. Rozen,et al. Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA–binding protein gene , 1995, Nature Genetics.

[7] D. Livingston. Immunoaffinity chromatography of proteins. , 1974, Methods in enzymology.

[8] U. K. Laemmli,et al. Cleavage of structural proteins during , 1970 .