Effects of three species endophytic fungi on Atractylodes lancea growth and its essential oil composition.

In a tissue culture experiment,this paper studied the effects of inoculating three species endophytic fungi on the growth and essential oil composition of Atractylodes lancea.After four weeks culture,inoculation of AL3(Sclerotium sp.)increased the average root number and dry mass per host plant by 92.7% and 62.9%(P0.01),respectively,while inoculation of AL4(Cunninghamella sp.)and AL12(Gilmaniella sp.)had less effect on host plant growth,compared with the control.Inoculating AL3,AL4,and AL12 increased the leaf PAL activity of A.lancea by 5.8,8.0,and 11.1 times,respectively(P0.01).The leaf POD,CAT and SOD activities were at the same levels as the control in treatment AL3,decreased by 9.1%,37.1%,and 9.7% in treatment AL4,and increased by 10.4%,21.2%,and 9.6% in treatment AL12,respectively.Inoculating AL3,AL4,and AL12 increased the total polyphenol content of A.lancea by 179.77%,86.19%,and 84.08%,respectively,and changed the relative percentage of four important components(atractylone,hinesol,β-eudesmol,and atractylodin)in essential oil.