Effects of transcranial focal electrical stimulation via tripolar concentric ring electrodes on pentylenetetrazole-induced seizures in rats

PURPOSE To study the effects of noninvasive transcranial focal electrical stimulation (TFS) via tripolar concentric ring electrodes (TCRE) on the electrographic and behavioral activity from pentylenetetrazole (PTZ)-induced seizures in rats. METHODS The TCREs were attached to the rat scalp. PTZ was administered and, after the first myoclonic jerk was observed, TFS was applied to the TFS treated group. The electroencephalogram (EEG) and behavioral activity were recorded and studied. RESULTS In the case of the TFS treated group, after TFS, there was a significant (p=0.001) decrease in power compared to the control group in delta, theta, and alpha frequency bands. The number of myoclonic jerks was significantly different (p=0.002) with median of 22 and 4.5 for the control group and the TFS treated groups, respectively. The duration of myoclonic activity was also significantly different (p=0.031) with median of 17.56 min for the control group versus 8.63 min for the TFS treated group. At the same time there was no significant difference in seizure onset latency and maximal behavioral seizure activity score between control and TFS treated groups. CONCLUSIONS TFS via TCREs interrupted PTZ-induced seizures and electrographic activity was reduced toward the "baseline." The significantly reduced electrographic power, number of myoclonic jerks, and duration of myoclonic activity of PTZ-induced seizures suggests that TFS may have an anticonvulsant effect.

[1]  W. Besio,et al.  Computer simulation and tank experimental verification of concentric ring electrodes , 2004, The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[2]  D. Shewmon,et al.  Trigeminal nerve stimulation for epilepsy , 2003, Neurology.

[3]  Robert S. Fisher,et al.  Animal models of the epilepsies , 1989, Brain Research Reviews.

[4]  W. Besio,et al.  Development of a Tri-polar Concentric Ring Electrode for Acquiring Accurate Laplacian Body Surface Potentials , 2006, Annals of Biomedical Engineering.

[5]  W. Theodore.  Brain stimulation for epilepsy , 2005, Nature Clinical Practice Neurology.

[6]  Nobuoki Eshima,et al.  ranscranial direct current stimulation decreases convulsions and spatial emory deficits following pilocarpine-induced status pilepticus in immature rats , 2010 .

[7]  Lippincott Williams Wilkins,et al.  A randomized controlled trial of chronic vagus nerve stimulation for treatment of medically intractable seizures , 1995, Neurology.

[8]  Mustafa M. Husain,et al.  Vagus nerve stimulation: a new tool for brain research and therapy∗ , 2000, Biological Psychiatry.

[9]  Richard Shiavi,et al.  Introduction to Applied Statistical Signal Analysis: Guide to Biomedical and Electrical Engineering Applications , 2006 .

[10]  Massimo Cincotta,et al.  Transcranial magnetic stimulation and epilepsy , 2003, Clinical Neurophysiology.

[11]  B. Uthman,et al.  Vagus nerve stimulation therapy for partial-onset seizures , 1998, Neurology.

[12]  Kanthaiah Koka,et al.  Effects of Noninvasive Transcutaneous Electrical Stimulation via Concentric Ring Electrodes on Pilocarpine‐induced Status Epilepticus in Rats , 2007, Epilepsia.

[13]  Alvaro Pascual-Leone,et al.  Seizure suppression by EEG-guided repetitive transcranial magnetic stimulation in the rat , 2008, Clinical Neurophysiology.

[14]  Walter G. Besio,et al.  Transcutaneous Focal Electrical Stimulation via Concentric Ring Electrodes Reduces Synchrony Induced by Pentylenetetrazole in beta and Gamma Bands in Rats , 2011, Int. J. Neural Syst..

[15]  Kristl Vonck,et al.  Long‐term amygdalohippocampal stimulation for refractory temporal lobe epilepsy , 2002, Annals of neurology.

[16]  H Buchner,et al.  Current density threshold for the stimulation of neurons in the motor cortex area , 2002, Bioelectromagnetics.

[17]  Philippe Kahane,et al.  Deep brain stimulation in epilepsy with particular reference to the subthalamic nucleus. , 2002, Epileptic disorders : international epilepsy journal with videotape.

[18]  Electroconvulsive therapy for seizure control: preliminary data in a new seizure generation and control model. , 2005, Frontiers in bioscience : a journal and virtual library.

[19]  Brian Litt,et al.  Electrical Stimulation of the Anterior Nucleus of the Thalamus for the Treatment of Intractable Epilepsy , 2004, Epilepsia.

[20]  M. Sarkisian.  Overview of the Current Animal Models for Human Seizure and Epileptic Disorders , 2001, Epilepsy & Behavior.

[21]  Á. Pascual-Leone,et al.  A Controlled Clinical Trial of Cathodal DC Polarization in Patients with Refractory Epilepsy , 2006, Epilepsia.

[22]  E. Wassermann.  Risk and safety of repetitive transcranial magnetic stimulation: report and suggested guidelines from the International Workshop on the Safety of Repetitive Transcranial Magnetic Stimulation, June 5-7, 1996. , 1998, Electroencephalography and clinical neurophysiology.

[23]  D. Spencer,et al.  Effect of an External Responsive Neurostimulator on Seizures and Electrographic Discharges during Subdural Electrode Monitoring , 2004, Epilepsia.

[24]  Walter G. Besio,et al.  Improvement of spatial selectivity and decrease of mutual information of tri-polar concentric ring electrodes , 2007, Journal of Neuroscience Methods.

[25]  Yasukazu Kajita,et al.  Suppression of secondary generalization of limbic seizures by stimulation of subthalamic nucleus in rats. , 2005, Journal of neurosurgery.

[26]  L. Cohen,et al.  Transcranial magnetic stimulation for the treatment of seizures: A controlled study , 2002, Neurology.

[27]  E. Ben-Menachem,et al.  Vagus Nerve Stimulation for Treatment of Partial Seizures: 1. A Controlled Study of Effect on Seizures , 1994, Epilepsia.

[28]  Steven Kay,et al.  Fundamentals Of Statistical Signal Processing , 2001 .

[29]  Erika E. Fanselow,et al.  Reduction of Pentylenetetrazole-Induced Seizure Activity in Awake Rats by Seizure-Triggered Trigeminal Nerve Stimulation , 2000, The Journal of Neuroscience.

[30]  Naoki Akamatsu,et al.  Decreased susceptibility to pentylenetetrazol-induced seizures after low-frequency transcranial magnetic stimulation in rats , 2001, Neuroscience Letters.

[31]  M. Nitsche,et al.  Anticonvulsant Effects of Transcranial Direct‐current Stimulation (tDCS) in the Rat Cortical Ramp Model of Focal Epilepsy , 2006, Epilepsia.

[32]  Rik Pintelon,et al.  Welch Method Revisited: Nonparametric Power Spectrum Estimation Via Circular Overlap , 2010, IEEE Transactions on Signal Processing.

[33]  Weizhong Dai,et al.  Tri-polar concentric ring electrode development for Laplacian electroencephalography , 2006, IEEE Transactions on Biomedical Engineering.

[34]  G. De Sarro,et al.  Effects of some AMPA receptor antagonists on the development of tolerance in epilepsy-prone rats and in pentylenetetrazole kindled rats. , 1999, European journal of pharmacology.

[35]  J. G. Webster,et al.  Analysis and Control of the Current Distribution under Circular Dispersive Electrodes , 1982, IEEE Transactions on Biomedical Engineering.

[36]  Mark Hallett,et al.  Transcranial magnetic stimulation: a revolution in clinical neurophysiology. , 2002, Journal of clinical neurophysiology : official publication of the American Electroencephalographic Society.

[37]  W. Besio,et al.  The Effects of Concentric Ring Electrode Electrical Stimulation on Rat Skin , 2010, Annals of Biomedical Engineering.

[38]  Robert S Fisher,et al.  Anticonvulsant effect of anterior thalamic high frequency electrical stimulation in the rat , 1997, Epilepsy Research.

[39]  Ana Luisa Velasco,et al.  Double‐blind, Randomized Controlled Pilot Study of Bilateral Cerebellar Stimulation for Treatment of Intractable Motor Seizures , 2005, Epilepsia.

[40]  T. Tcheng,et al.  Preemptive Low‐frequency Stimulation Decreases the Incidence of Amygdala‐kindled Seizures , 2005, Epilepsia.

[41]  E. Bebin,et al.  Efficacy of Vagal Nerve Stimulation in Children with Medically Refractory Epilepsy , 2000, Neurosurgery.