Research on the mechanism of HP mediated PI3K/AKT/GSK3β pathways in gastric cancer.

OBJECTIVE This study aimed to investigate the mechanism of Helicobacter pylori (HP) mediated PI3K/AKT/GSK3β signal pathways in the occurrence of gastric cancer. PATIENTS AND METHODS For this purpose, a total of 90 patients were selected; 30 were suffering from gastric cancer and gastric ulcer (observation group I), 30 were suffering from pure gastric ulcer (observation group II) and 30 were with chronic gastritis (control group), and the lesion tissues were extracted by using endoscopy. Then, rapid urease test, RT-PCR and Western blot tests were conducted to detect the positivity rate of HP infection, the expression levels of PI3K/AKT/GSK3β mRNA and protein respectively. RESULTS The results showed that the positivity rate of HP infection, expression level of the PI3K/AKT/GSK3β mRNA and protein from the samples of the observation group I were significantly higher than other two groups (p-value <0.05). CONCLUSIONS HP may mediate the PI3K/AKT/GSK3β signal pathways in the occurrence of gastric cancer.

[1]  T. Meyer,et al.  Gastric cancer pathogenesis , 2016, Helicobacter.

[2]  C. O'Morain,et al.  Treatment of Helicobacter pylori infection 2016 , 2016, Helicobacter.

[3]  D. Morgan,et al.  Hydrogen Metabolism in Helicobacter pylori Plays a Role in Gastric Carcinogenesis through Facilitating CagA Translocation , 2016, mBio.

[4]  Yu-Jen Wu,et al.  Sinularin Induces Apoptosis through Mitochondria Dysfunction and Inactivation of the pI3K/Akt/mTOR Pathway in Gastric Carcinoma Cells , 2016, Marine drugs.

[5]  J. Bae,et al.  Helicobacter pylori Infection and Risk of Gastric Cancer in Korea: A Quantitative Systematic Review , 2016, Journal of preventive medicine and public health = Yebang Uihakhoe chi.

[6]  Minhu Chen,et al.  Gastrokine-2 suppresses epithelial mesenchymal transition through PI3K/AKT/GSK3β signaling in gastric cancer , 2016, Tumor Biology.

[7]  Z. Xie,et al.  Expression level of microRNA-195 in the serum of patients with gastric cancer and its relationship with the clinicopathological staging of the cancer. , 2016, European review for medical and pharmacological sciences.

[8]  S. Turanli,et al.  The Effect of Helicobacter pylori on Gastric Cancer Treated with Adjuvant Chemotherapy After Curative Resection , 2015, Indian Journal of Surgery.

[9]  Ji Hyun Lee,et al.  Fibulin-3-mediated inhibition of epithelial-to-mesenchymal transition and self-renewal of ALDH+ lung cancer stem cells through IGF1R signaling , 2014, Oncogene.

[10]  Hongli Li,et al.  Nir1 promotes invasion of breast cancer cells by binding to chemokine (C-C motif) ligand 18 through the PI3K/Akt/GSK3β/Snail signalling pathway. , 2013, European journal of cancer.

[11]  Jun Du,et al.  PI3K/Akt-dependent phosphorylation of GSK3β and activation of RhoA regulate Wnt5a-induced gastric cancer cell migration. , 2013, Cellular signalling.

[12]  T. A. Nagy,et al.  Regulation of p53 tumor suppressor by Helicobacter pylori in gastric epithelial cells. , 2010, Gastroenterology.

[13]  Z. Ge,et al.  Role of bile reflux and Helicobacter pylori infection on inflammation of gastric remnant after distal gastrectomy , 2008, Journal of digestive diseases.

[14]  E. Sasaki,et al.  Production of Reactive Oxygen Species in Peripheral Blood is Increased in Individuals with Helicobacter pylori Infection and Decreased after its Eradication , 2006, Helicobacter.

[15]  Y. Matsuda,et al.  Significance of Akt Phosphorylation on Tumor Growth and Vascular Endothelial Growth Factor Expression in Human Gastric Carcinoma , 2006, Pathobiology.

[16]  H-l Chen,et al.  Activation of Akt and ERK signalling pathways induced by etoposide confer chemoresistance in gastric cancer cells. , 2006, Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver.

[17]  F. Angelico,et al.  Gastric Cancer Precursor Lesions and Helicobacter pylori Infection in Patients with Partial Gastrectomy for Peptic Ulcer , 2005, World Journal of Surgery.

[18]  M. Oshimura,et al.  PI3K-Akt pathway: Its functions and alterations in human cancer , 2004, Apoptosis.

[19]  J. Roth,et al.  Clinical evaluation of adenoviral-mediated p53 gene transfer: review of INGN 201 studies. , 2001, Seminars in oncology.

[20]  E. Furth,et al.  Altered gastric epithelial cell kinetics in Helicobacter pylori‐associated intestinal metaplasia: Implications for gastric carcinogenesis , 2000, International journal of cancer.

[21]  S. Nordling,et al.  Does Helicobacter pylori in the gastric stump increase the cancer risk after certain reconstruction types? , 1997, Anticancer research.

[22]  Thomas K. Hunt,et al.  ON THE TREATMENT OF , 1846 .

[23]  K. Dodig-Ćurković,et al.  Helicobacter pylori, zinc and iron in oxidative stress-induced injury of gastric mucosa. , 2009, Mini reviews in medicinal chemistry.

[24]  眞下 勝行.  Production of reactive oxygen species in peripheral blood is increased in individuals with Helicobacter pylori infection and decreased after its eradication , 2007 .

[25]  M. Osaki,et al.  Inhibition of the PI3K-Akt signaling pathway enhances the sensitivity of Fas-mediated apoptosis in human gastric carcinoma cell line, MKN-45 , 2003, Journal of Cancer Research and Clinical Oncology.