Evaluation of chitosan nano dressing for wound healing: characterization, in vitro and in vivo studies.

In our present investigation, a ternary nano dressing consists of titanium dioxide nano particle loaded chitosan-pectin was prepared to evaluate biocompatibility, antimicrobial and in vivo wound healing properties. The photoactive property of TiO₂ based materials makes it important candidate for numerous medical applications. Chitosan can be easily processed into membranes, gels, nanofibers, beads, nanoparticles, scaffolds, and sponge forms that can be used in wound healing applications. Pectin acts as a natural prophylactic substance against poisoning with toxic cations and its styptic and curing effects are well documented in healing ointments. The characterizations of prepared nano dressing were made by FTIR, TGA, DSC, SEM and TEM. The physicochemical parameters of nano dressing were evaluated by various techniques, namely, the Whole blood clotting test, haemolysis ratio measurement, cytotoxicity test using NIH3T3 and L929 fibroblast cells. The in vivo open excision-type wound healing efficiency of prepared nano dressing and its comparison with conventional gauze were evaluated by measuring wound contraction and histological examinations in adult male albino rats. The synergistic effects of nano dressing such as good antibacterial ability, high swelling properties, high water vapour transmission rate (WVTR), excellent hydrophilic nature, biocompatibility, wound appearance, wound closure rate and histological study through in vivo test makes it a suitable candidate for wound healing applications.

[1]  S. Nair,et al.  Biomaterials based on chitin and chitosan in wound dressing applications. , 2011, Biotechnology advances.

[2]  Shantikumar V. Nair,et al.  Flexible and microporous chitosan hydrogel/nano ZnO composite bandages for wound dressing: in vitro and in vivo evaluation. , 2012, ACS applied materials & interfaces.

[3]  K Madhumathi,et al.  Wet chemical synthesis of chitosan hydrogel-hydroxyapatite composite membranes for tissue engineering applications. , 2009, International journal of biological macromolecules.

[4]  C. Samyn,et al.  Characterization of colon-specific azo polymers: A study of the swelling propertoes and the permeability of isolated polymer films , 1994 .

[5]  Changyou Gao,et al.  Chitosan–hyaluronic acid hybrid film as a novel wound dressing: in vitro and in vivo studies , 2007 .

[6]  Lawrence E Murr,et al.  Comparative in vitro cytotoxicity assessment of some manufacturednanoparticulate materials characterized by transmissionelectron microscopy , 2005 .

[7]  Yi-Wen Chen,et al.  Composite nano-titanium oxide-chitosan artificial skin exhibits strong wound-healing effect-an approach with anti-inflammatory and bactericidal kinetics. , 2008, Macromolecular bioscience.

[8]  Yongjun Li,et al.  Comparative study on the acute pulmonary toxicity induced by 3 and 20nm TiO(2) primary particles in mice. , 2007, Environmental toxicology and pharmacology.

[9]  J. West,et al.  Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. , 2006, Toxicological sciences : an official journal of the Society of Toxicology.

[10]  Navid B. Saleh,et al.  Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity. , 2006, Environmental science & technology.

[11]  Ting-Yun Kuo,et al.  Novel chitosan-pectin composite membranes with enhanced strength, hydrophilicity and controllable disintegration. , 2010 .

[12]  T. Tan,et al.  The bactericidal and mildew-proof activity of a TiO2–chitosan composite , 2011 .

[13]  Z. Chai,et al.  Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration. , 2007, Toxicology letters.

[14]  Zhaoliang Zhang,et al.  Cytotoxicity and biocompatibility evaluation of N,O-carboxymethyl chitosan/oxidized alginate hydrogel for drug delivery application. , 2012, International journal of biological macromolecules.

[15]  G. Abraham,et al.  Development of new hydroactive dressings based on chitosan membranes: characterization and in vivo behavior. , 2003, Journal of biomedical materials research. Part A.

[16]  M. Amiji.  Permeability and blood compatibility properties of chitosan-poly(ethylene oxide) blend membranes for haemodialysis. , 1995, Biomaterials.

[17]  V. Grassian,et al.  Inhalation Exposure Study of Titanium Dioxide Nanoparticles with a Primary Particle Size of 2 to 5 nm , 2006, Environmental health perspectives.

[18]  J. Evans,et al.  The preclinical evaluation of the water vapour transmission rate through burn wound dressings. , 1987, Biomaterials.

[19]  A. Gedanken,et al.  Enhanced inactivation of bacteria by metal‐oxide nanoparticles combined with visible light irradiation , 2011, Lasers in surgery and medicine.

[20]  L. Murr,et al.  Cytotoxic effects of aggregated nanomaterials. , 2007, Acta biomaterialia.

[21]  R. Dey,et al.  Synthesis, characterization, and blood compatibility of polyamidoamines copolymers. , 2003, Biomaterials.

[22]  K. Chennazhi,et al.  Fabrication of chitin-chitosan/nano TiO2-composite scaffolds for tissue engineering applications. , 2011, International journal of biological macromolecules.

[23]  Tejal A Desai,et al.  The effect of TiO2 nanotubes on endothelial function and smooth muscle proliferation. , 2009, Biomaterials.

[24]  Robert Gurny,et al.  Injectable rhBMP-2-loaded chitosan hydrogel composite: osteoinduction at ectopic site and in segmental long bone defect. , 2011, Journal of biomedical materials research. Part A.

[25]  I. Alsarra.  Chitosan topical gel formulation in the management of burn wounds. , 2009, International journal of biological macromolecules.

[26]  Shantikumar V. Nair,et al.  Biomedical applications of chitin and chitosan based nanomaterials—A short review , 2010 .

[27]  Kazuhiro Yoshida,et al.  Cytotoxicity of Metal and Ceramic Particles in Different Sizes , 2003 .

[28]  B. Helgason,et al.  In vitro bioactivity of different degree of deacetylation chitosan, a potential coating material for titanium implants. , 2012, Journal of Biomedical Materials Research. Part A.

[29]  P. Dutta,et al.  Preparation, circular dichroism induced helical conformation and optical property of chitosan acid salt complexes for biomedical applications. , 2009, International journal of biological macromolecules.

[30]  Alok R. Ray,et al.  Biomedical Applications of Chitin, Chitosan, and Their Derivatives , 2000 .

[31]  H. Fan,et al.  Asymmetric polyurethane membrane with in situ‐generated nano‐TiO2 as wound dressing , 2011 .

[32]  Q. Fu,et al.  Preparation and properties of chitosan nanocomposites with nanofillers of different dimensions , 2009 .

[33]  Dusan Losic,et al.  Biocompatible polymer coating of titania nanotube arrays for improved drug elution and osteoblast adhesion. , 2012, Acta biomaterialia.

[34]  S. Wongnawa,et al.  Mixed amorphous and nanocrystalline TiO2 powders prepared by sol–gel method: Characterization and photocatalytic study , 2008 .

[35]  J. Dutta,et al.  Chitin and chitosan: Chemistry, properties and applications , 2004 .

[36]  P. Aebischer,et al.  Chitosan as a matrix for mammalian cell encapsulation. , 1994, Biomaterials.

[37]  Sungho Jin,et al.  Enhanced cellular mobility guided by TiO2 nanotube surfaces. , 2008, Nano letters.