Systemin: a polypeptide signal for plant defensive genes.

Damage to leaves of several plant species by herbivores or by other mechanical wounding induces defense gene activation throughout the plants within hours. An 18-amino acid polypeptide, called systemin, has been isolated from tomato leaves that is a powerful inducer of over 15 defensive genes when supplied to the tomato plants at levels of fmol/plant. Systemin is readily transported from wound sites and is considered to be the primary systemic signal. The polypeptide is processed from a 200-amino acid precursor called prosystemin, analogous to polypeptide hormones in animals. However, the plant prohormone does not possess typical dibasic cleavage sites, nor does it contain a signal sequence or any typical membrane-spanning regions. The signal transduction pathway that mediates systemin signaling involves linolenic acid release from membranes and subsequent conversion to jasmonic acid, a potent activator of defense gene transcription. The pathway exhibits analogies to arachidonic acid/prostaglandin signaling in animals that leads to inflammatory and acute phase responses.

[1]  Ryan,et al.  Wound signaling in tomato plants. Evidence that aba is not a primary signal for defense gene activation , 1998, Plant physiology.

[2]  A. Schaller.  Action of proteolysis-resistant systemin analogues in wound signalling. , 1998, Phytochemistry.

[3]  Y. Matsubayashi,et al.  Phytosulfokine-alpha, a sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high- and low-affinity binding sites. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[4]  T. Jacinto,et al.  Tomato prosystemin promoter confers wound-inducible, vascular bundle-specific expression of the β-glucuronidase gene in transgenic tomato plants , 1997, Planta.

[5]  Kathleen Y. Lee,et al.  Post‐transcriptional gene silencing of ACC synthase in tomato results from cytoplasmic RNA degradation , 1997 .

[6]  M. E. Hoyos,et al.  Rapid and Transient Activation of a Myelin Basic Protein Kinase in Tobacco Leaves Treated with Harpin from Erwinia amylovora , 1997, Plant physiology.

[7]  J. Stratmann,et al.  Myelin basic protein kinase activity in tomato leaves is induced systemically by wounding and increases in response to systemin and oligosaccharide elicitors. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[8]  H. Nam,et al.  Systemic elevation of phosphatidic acid and lysophospholipid levels in wounded plants , 1997 .

[9]  E. Weiler.  Octadecanoid-Mediated Signal Transduction in Higher Plants , 1997, Naturwissenschaften.

[10]  C. Ryan,et al.  A Gene Encoding a Chloroplast-Targeted Lipoxygenase in Tomato Leaves Is Transiently Induced by Wounding, Systemin, and Methyl Jasmonate , 1997, Plant physiology.

[11]  H. Kende,et al.  The Five "Classical" Plant Hormones. , 1997, The Plant cell.

[12]  H. Leyser,et al.  Ethylene as a Signal Mediating the Wound Response of Tomato Plants , 1996, Science.

[13]  G. Howe,et al.  An octadecanoid pathway mutant (JL5) of tomato is compromised in signaling for defense against insect attack. , 1996, The Plant cell.

[14]  G. Howe,et al.  Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[15]  D. McCarty,et al.  CRINKLY4: A TNFR-Like Receptor Kinase Involved in Maize Epidermal Differentiation , 1996, Science.

[16]  Y. Matsubayashi,et al.  Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[17]  T. Bisseling,et al.  Modification of Phytohormone Response by a Peptide Encoded by ENOD40 of Legumes and a Nonlegume , 1996, Science.

[18]  J. Browse,et al.  Intracellular Levels of Free Linolenic and Linoleic Acids Increase in Tomato Leaves in Response to Wounding , 1996, Plant physiology.

[19]  S. Abraham,et al.  Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-α , 1996, Nature.

[20]  E. Johannes,et al.  Systemin transiently depolarizes the tomato mesophyll cell membrane and antagonizes fusicoccin‐induced extracellular acidification of mesophyll tissue , 1996 .

[21]  N. Mitsukawa,et al.  The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats. , 1996, The Plant cell.

[22]  P. Low,et al.  The oxidative burst in plant defense: Function and signal transduction , 1996 .

[23]  D. Braun,et al.  Plant transmembrane receptors: new pieces in the signaling puzzle. , 1996, Trends in biochemical sciences.

[24]  C. Ryan,et al.  Systemin – a polypeptide defense signal in plants , 1996, BioEssays : news and reviews in molecular, cellular and developmental biology.

[25]  H. Yoshioka,et al.  The oxidative burst protects plants against pathogen attack: mechanism and role as an emergency signal for plant bio-defence--a review. , 1996, Gene.

[26]  H. Sano,et al.  Tobacco MAP Kinase: A Possible Mediator in Wound Signal Transduction Pathways , 1995, Science.

[27]  D. Stevens.  Could nonsteroidal antiinflammatory drugs (NSAIDs) enhance the progression of bacterial infections to toxic shock syndrome? , 1995, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[28]  Y. Ito,et al.  Cutting activates a 46-kilodalton protein kinase in plants. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[29]  E. Weiler,et al.  Salicylic Acid Inhibits Synthesis of Proteinase Inhibitors in Tomato Leaves Induced by Systemin and Jasmonic Acid , 1995, Plant physiology.

[30]  W. C. Johnson,et al.  Systemin has the characteristics of a poly(L-proline) II type helix , 1995 .

[31]  H. Kalbitzer,et al.  Mechanism of the activation of proteinase inhibitor synthesis by systemin involves beta-sheet structure, a specific DNA-binding protein domain. , 1995, Journal of structural biology.

[32]  E. Weiler,et al.  Oligogalacturonides and chitosan activate plant defensive genes through the octadecanoid pathway. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[33]  T. Boller,et al.  Systemin induces rapid ion fluxes and ethylene biosynthesis in Lycopersicon peruvianum cells , 1995 .

[34]  C. Ryan,et al.  Identification of a 50-kDa systemin-binding protein in tomato plasma membranes having Kex2p-like properties. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[35]  G. Pearce,et al.  Overexpression of the prosystemin gene in transgenic tomato plants generates a systemic signal that constitutively induces proteinase inhibitor synthesis. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[36]  E. Farmer,et al.  Diethyldithiocarbamic Acid Inhibits the Octadecanoid Signaling Pathway for the Wound Induction of Proteinase Inhibitors in Tomato Leaves , 1994 .

[37]  K. Ferrell,et al.  Molecular cloning and expression of a gamma-interferon-inducible activator of the multicatalytic protease. , 1994, The Journal of biological chemistry.

[38]  Claudio Realini,et al.  KEKE motifs , 1994, FEBS letters.

[39]  C. Ryan,et al.  A Sulfhydryl Reagent Modulates Systemic Signaling for Wound-Induced and Systemin-Induced Proteinase Inhibitor Synthesis , 1994, Plant physiology.

[40]  J. Gauldie,et al.  The acute phase response. , 1994, Immunology today.

[41]  A. Whitehead,et al.  The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A protein. , 1994, Immunology today.

[42]  N. Seidah,et al.  The family of subtilisin/kexin like pro-protein and pro-hormone convertases: divergent or shared functions. , 1994, Biochimie.

[43]  D. Boulter,et al.  Insect pest control by copying nature using genetically engineered crops. , 1993, Phytochemistry.

[44]  C. Bolter.  Methyl Jasmonate Induces Papain Inhibitor(s) in Tomato Leaves , 1993, Plant physiology.

[45]  L. Walling,et al.  Leucine aminopeptidase: an inducible component of the defense response in Lycopersicon esculentum (tomato). , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[46]  P. Low,et al.  Characterization of the Oligogalacturonide-Induced Oxidative Burst in Cultured Soybean (Glycine max) Cells , 1993, Plant physiology.

[47]  Roger J. Davis,et al.  cPLA2 is phosphorylated and activated by MAP kinase , 1993, Cell.

[48]  G. Pearce,et al.  Structure-activity of deleted and substituted systemin, an 18-amino acid polypeptide inducer of plant defensive genes. , 1993, The Journal of biological chemistry.

[49]  L. Willmitzer,et al.  General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding. , 1992, The Plant cell.

[50]  G. Pearce,et al.  Proton NMR assignments of systemin , 1992, Journal of protein chemistry.

[51]  G. Pearce,et al.  Structure, expression, and antisense inhibition of the systemin precursor gene. , 1992, Science.

[52]  M. Zenk,et al.  Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[53]  E. Farmer,et al.  Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-Inducible Proteinase Inhibitors. , 1992, The Plant cell.

[54]  L. Willmitzer,et al.  Abscisic Acid Mediates Wound Induction but Not Developmental-Specific Expression of the Proteinase Inhibitor II Gene Family. , 1991, The Plant cell.

[55]  G. Pearce,et al.  A Polypeptide from Tomato Leaves Induces Wound-Inducible Proteinase Inhibitor Proteins , 1991, Science.

[56]  C. Ryan.  Protease Inhibitors in Plants: Genes for Improving Defenses Against Insects and Pathogens , 1990 .

[57]  L. Willmitzer,et al.  Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[58]  R. Thornburg,et al.  Auxin Levels Regulate the Expression of a Wound-Inducible Proteinase Inhibitor II-Chloramphenicol Acetyl Transferase Gene Fusion in Vitro and in Vivo. , 1989, Plant physiology.

[59]  J. Sipe,et al.  The acute phase response in gout. , 1987, The Journal of rheumatology.

[60]  C. Ryan,et al.  Isolation and characterization of a wound-induced trypsin inhibitor from alfalfa leaves. , 1984, Biochemistry.

[61]  B. Vick,et al.  Biosynthesis of jasmonic Acid by several plant species. , 1984, Plant physiology.

[62]  C. Ryan,et al.  Isolation and properties of carboxypeptidase from leaves of wounded tomato plants , 1980 .

[63]  M. Caton,et al.  Prostaglandins and thromboxanes. , 1979, Progress in medicinal chemistry.

[64]  B. Samuelsson.  Prostaglandins and Thromboxanes , 1977, NATO Advanced Study Institutes Series.

[65]  C. Ryan.  Assay and Biochemical Properties of the Proteinase Inhibitor-inducing Factor, a Wound Hormone. , 1974, Plant physiology.

[66]  Michael D Greenfield,et al.  Wound-Induced Proteinase Inhibitor in Plant Leaves: A Possible Defense Mechanism against Insects , 1972, Science.