Dental plaque microcosm biofilm behavior on calcium phosphate nanocomposite with quaternary ammonium.

OBJECTIVES Half of dental restorations fail in 10 years, with secondary caries as the main reason. Calcium phosphate composites could remineralize tooth lesions. The objectives of this study were to: (1) impart antibacterial activity to a composite with nanoparticles of amorphous calcium phosphate (NACP); and (2) investigate the effect of quaternary ammonium dimethacrylate (QADM) on mechanical and dental plaque microcosm biofilm properties for the first time. METHODS The NACP and glass particles were filled into a dental resin that contained bis(2-methacryloyloxy-ethyl) dimethyl-ammonium bromide, the QADM. NACP nanocomposites containing 0%, 7%, 14%, and 17.5% of QADM by mass, respectively, were photo-cured. A commercial composite with no antibacterial activity was used as control. Mechanical properties were measured in three-point flexure. A human saliva microcosm model was used to grow biofilms on composites. Live/dead assay, metabolic activity, colony-forming unit (CFU) counts, and lactic acid production of biofilms on the composites were measured. RESULTS Increasing QADM mass fraction monotonically reduced the biofilm viability, CFU and lactic acid. Biofilms on NACP nanocomposite with 17.5% QADM had metabolic activity that was 30% that on a commercial composite control (p<0.05). Total microorganisms, total streptococci, and mutans streptococci CFU counts (mean ± sd; n = 6) on composite control was 6-fold those on NACP +17.5% QADM nanocomposite. Composite control had long strings of bacterial cells with normal short-rod shapes, while some cells on NACP-QADM nanocomposites disintegrated into pieces. Adding QADM to NACP did not decrease the composite strength and elastic modulus, which matched (p>0.1) those of a commercial composite without Ca-PO(4) or antibacterial activity. SIGNIFICANCE A dental plaque microcosm model was used to evaluate the novel NACP-QADM nanocomposite. The nanocomposite greatly reduced the biofilm viability, metabolic activity and lactic acid, while its mechanical properties matched those of a commercial composite. NACP-QADM nanocomposite with calcium phosphate fillers, good mechanical properties and a strong antibacterial activity may have potential for anti-biofilm and anti-caries restorations.

[1]  Xuedong Zhou,et al.  Effect of Galla Chinensis on Growth and Metabolism of Microcosm Biofilms , 2011, Caries Research.

[2]  M. Peltz,et al.  Nano DCPA-Whisker Composites with High Strength and Ca and PO4 Release , 2006, Journal of dental research.

[3]  D. Watts,et al.  Photo-polymerization shrinkage-stress kinetics in resin-composites: methods development. , 2003, Dental materials : official publication of the Academy of Dental Materials.

[4]  M. Torii,et al.  Incorporation of Bacterial Inhibitor into Resin Composite , 1994, Journal of dental research.

[5]  D. Watts,et al.  Hygroscopic dimensional changes of self-adhering and new resin-matrix composites during water sorption/desorption cycles. , 2011, Dental materials : official publication of the Academy of Dental Materials.

[6]  J. M. Cate.  Biofilms, a new approach to the microbiology of dental plaque , 2006, Odontology.

[7]  D. Sarrett.  Clinical challenges and the relevance of materials testing for posterior composite restorations. , 2005, Dental materials : official publication of the Academy of Dental Materials.

[8]  S. Imazato.  Antibacterial properties of resin composites and dentin bonding systems. , 2003, Dental materials : official publication of the Academy of Dental Materials.

[9]  Roland Frankenberger,et al.  Nanohybrid vs. fine hybrid composite in extended Class II cavities after six years. , 2011, Dental materials : official publication of the Academy of Dental Materials.

[10]  J. Featherstone,et al.  The Continuum of Dental Caries—Evidence for a Dynamic Disease Process , 2004, Journal of dental research.

[11]  Limin Sun,et al.  Nanocomposite containing amorphous calcium phosphate nanoparticles for caries inhibition. , 2011, Dental materials : official publication of the Academy of Dental Materials.

[12]  L. Ni,et al.  An in vitro synergetic evaluation of the use of nisin and sodium fluoride or chlorhexidine against Streptococcus mutans , 2011, Peptides.

[13]  L. Wong,et al.  Human dental plaque microcosm biofilms: effect of nutrient variation on calcium phosphate deposition and growth. , 2007, Archives of oral biology.

[14]  M. Sela,et al.  Accumulation of Streptococcus mutans on light-cured composites and amalgam: an in vitro study. , 1998, Journal of esthetic dentistry.

[15]  S. Takashiba,et al.  Antibacterial effect of bactericide immobilized in resin matrix. , 2009, Dental materials : official publication of the Academy of Dental Materials.

[16]  D. Deng,et al.  Demineralization of Dentin by Streptococcus mutans Biofilms Grown in the Constant Depth Film Fermentor , 2003, Caries Research.

[17]  M. Peltz,et al.  Nano Dicalcium Phosphate Anhydrous-Whisker Composites With High Strength and Ca and PO4 Release , 2006 .

[18]  J. Y. Thompson,et al.  Effect of novel filler particles on the mechanical and wear properties of dental composites. , 2002, Dental materials : official publication of the Academy of Dental Materials.

[19]  J. Pratten,et al.  Characterization of in vitro oral bacterial biofilms by traditional and molecular methods. , 2003, Oral microbiology and immunology.

[20]  J. Pratten,et al.  The effect of orthodontic bonding materials on dental plaque accumulation and composition in vitro. , 2003, Biomaterials.

[21]  P. Spencer,et al.  Adhesive phase separation at the dentin interface under wet bonding conditions. , 2002, Journal of biomedical materials research.

[22]  C. Sissons,et al.  Development and characterization of a simple perfused oral microcosm , 2005, Journal of applied microbiology.

[23]  L. Sun,et al.  Strong Nanocomposites with Ca, PO4, and F Release for Caries Inhibition , 2010, Journal of dental research.

[24]  D. Škrtić,et al.  In vitro remineralization of enamel by polymeric amorphous calcium phosphate composite: quantitative microradiographic study. , 2009, Dental materials : official publication of the Academy of Dental Materials.

[25]  Xuedong Zhou,et al.  Antibacterial amorphous calcium phosphate nanocomposite with quaternary ammonium salt and silver nanoparticles , 2012 .

[26]  J. M. ten Cate,et al.  Relationship between Gap Size and Dentine Secondary Caries Formation Assessed in a Microcosm Biofilm Model , 2009, Caries Research.

[27]  K. Whang,et al.  Development of an antimicrobial resin--a pilot study. , 2011, Dental materials : official publication of the Academy of Dental Materials.

[28]  P. Frost.  An Audit on the Placement and Replacement of Restorations in a General Dental Practice , 2002, Primary dental care : journal of the Faculty of General Dental Practitioners.

[29]  B. Lim,et al.  Reduction of polymerization contraction stress for dental composites by two-step light-activation. , 2002, Dental materials : official publication of the Academy of Dental Materials.

[30]  S. H. Dickens,et al.  Mechanical properties and biochemical activity of remineralizing resin-based Ca-PO4 cements. , 2003, Dental materials : official publication of the Academy of Dental Materials.

[31]  J. Antonucci,et al.  Synthesis and characterization of dimethacrylates containing quaternary ammonium functionalities for dental applications. , 2012, Dental materials : official publication of the Academy of Dental Materials.

[32]  L. Rodrigues,et al.  Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries. , 2009, European journal of oral sciences.

[33]  M. Peters,et al.  In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond. , 2006, Dental materials : official publication of the Academy of Dental Materials.

[34]  A. McBain,et al.  Chapter 4: In vitro biofilm models: an overview. , 2009, Advances in applied microbiology.

[35]  J. Stansbury,et al.  Impact of Curing Protocol on Conversion and Shrinkage Stress , 2005, Journal of dental research.

[36]  R. Sakaguchi,et al.  Review of the current status and challenges for dental posterior restorative composites: clinical, chemistry, and physical behavior considerations. Summary of discussion from the Portland Composites Symposium (POCOS) June 17-19, 2004, Oregon Health and Science University, Portland, Oregon. , 2005, Dental materials : official publication of the Academy of Dental Materials.

[37]  T. Maeda,et al.  Syntheses and antimicrobial activities of a series of new bis-quaternary ammonium compounds. , 2006, European journal of medicinal chemistry.

[38]  K. Agee,et al.  The Inhibitory Effects of Quaternary Ammonium Methacrylates on Soluble and Matrix-bound MMPs , 2011, Journal of dental research.

[39]  Satoshi Imazato,et al.  Bio-active restorative materials with antibacterial effects: new dimension of innovation in restorative dentistry. , 2009, Dental materials journal.

[40]  Xiaoming Xu,et al.  Formulation and characterization of a novel fluoride-releasing dental composite. , 2006, Dental materials : official publication of the Academy of Dental Materials.

[41]  D. Zero,et al.  A New in vitro Model to Study the Relationship of Gap Size and Secondary Caries , 2007, Caries Research.

[42]  I A Mjör,et al.  An Overview of Reasons for the Placement and Replacement of Restorations , 2001, Primary dental care : journal of the Faculty of General Dental Practitioners.

[43]  U Blunck,et al.  Quality of dental restorations. FDI Commission Project 2-95. , 2001, International dental journal.

[44]  B. Nyvad,et al.  Caries Ecology Revisited: Microbial Dynamics and the Caries Process , 2008, Caries Research.

[45]  A. Domb,et al.  Antibacterial activity of dental composites containing quaternary ammonium polyethylenimine nanoparticles against Streptococcus mutans. , 2006, Biomaterials.

[46]  M. Torii,et al.  Incorporation of Antibacterial Monomer MDPB into Dentin Primer , 1997, Journal of dental research.

[47]  Limin Sun,et al.  Mechanical and acid neutralizing properties and bacteria inhibition of amorphous calcium phosphate dental nanocomposite. , 2011, Journal of biomedical materials research. Part B, Applied biomaterials.

[48]  S. Socransky,et al.  Identification of early microbial colonizers in human dental biofilm , 2004, Journal of applied microbiology.

[49]  Sheng Lin-Gibson,et al.  Antibacterial amorphous calcium phosphate nanocomposites with a quaternary ammonium dimethacrylate and silver nanoparticles. , 2012, Dental materials : official publication of the Academy of Dental Materials.

[50]  J. Y. Thompson,et al.  A characterization of first-generation flowable composites. , 1998, Journal of the American Dental Association.

[51]  D. Škrtić,et al.  Physicochemical evaluation of bioactive polymeric composites based on hybrid amorphous calcium phosphates. , 2000, Journal of biomedical materials research.

[52]  Jun Zhao,et al.  Preparation and evaluation of a novel glass-ionomer cement with antibacterial functions. , 2011, Dental materials : official publication of the Academy of Dental Materials.

[53]  Jack L Ferracane,et al.  Resin composite--state of the art. , 2011, Dental materials : official publication of the Academy of Dental Materials.

[54]  Abraham J Domb,et al.  An in vitro quantitative antibacterial analysis of amalgam and composite resins. , 2007, Journal of dentistry.

[55]  J. M. ten Cate,et al.  The effect of triclosan toothpaste on enamel demineralization in a bacterial demineralization model. , 2000, The Journal of antimicrobial chemotherapy.

[56]  J. Drummond,et al.  Degradation, Fatigue, and Failure of Resin Dental Composite Materials , 2008, Journal of dental research.

[57]  J. Drummond,et al.  Cyclic loading of notched dental composite specimens. , 2010, Dental Materials.

[58]  Sai Ma,et al.  Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans. , 2009, Journal of dentistry.

[59]  I. Sideridou,et al.  Physical properties of current dental nanohybrid and nanofill light-cured resin composites. , 2011, Dental materials : official publication of the Academy of Dental Materials.