Reductive Microbial Dechlorination of Indigenous Polychlorinated Biphenyls in Soil Using a Sediment‐Free Inoculum

In laboratory experiments, unagitated soil slurry bioreactors inoculated with microorganisms extracted from polychlorinated biphenyl‐contaminated (PCBs) sediments from the Hudson River were used to anaerobically dechlorinate PCBs. The onset of dechlorination activity was accelerated by the addition of certain organic acids (pyruvate and maleate) and single congeners (2,3,6‐trichlorobiphenyl). Dechlorination was observed under several working conditions after 19 weeks of incubation with PCB‐contaminated soil and nutrient solution. Best results showed a drop in average chlorine content from 4.3 to 3.6 chlorines per biphenyl due to a loss of m‐chlorines. Soil used for these experiments was obtained from a PCB‐contaminated (weathered Aroclor 1248) site at an electric power substation. Dechlorination was observed with no sediment particles or other matrix being added.

[1]  J F Brown,et al.  Determination of PCB Metabolic, Excretion, and Accumulation Rates for Use as Indicators of Biological Response and Relative Risk. , 1994, Environmental science & technology.

[2]  W. Williams.  Microbial reductive dechlorination of trichlorobiphenyls in anaerobic sediment slurries. , 1994, Environmental science & technology.

[3]  D. Abramowicz,et al.  Factors influencing the rate of polychlorinated biphenyls dechlorination in Hudson River sediments , 1993 .

[4]  J. Tiedje,et al.  Microbial reductive dehalogenation. , 1992, Microbiological reviews.

[5]  D. Bedard,et al.  Reductive ortho and meta Dechlorination of a Polychlorinated Biphenyl Congener by Anaerobic Microorganisms , 1991, Applied and environmental microbiology.

[6]  T. Vogel,et al.  Effects of Organic Substrates on Dechlorination of Aroclor 1242 in Anaerobic Sediments , 1990, Applied and environmental microbiology.

[7]  James M. Tiedje,et al.  Dechlorination of Four Commercial Polychlorinated Biphenyl Mixtures (Aroclors) by Anaerobic Microorganisms from Sediments , 1990, Applied and environmental microbiology.

[8]  J. Duinker,et al.  Complete characterization of polychlorinated biphenyl congeners in commercial Aroclor and Clophen mixtures by multidimensional gas chromatography-electron capture detection , 1989 .

[9]  Ralph J. May,et al.  Environmental dechlorination of PCBs , 1987 .

[10]  K. Klasson,et al.  Sequential anaerobic-aerobic biodegradation of PCBs in soil slurry microcosms , 1996, Applied biochemistry and biotechnology.

[11]  D. Abramowicz.  Aerobic and Anaerobic Biodegradation of PCBs: A Review , 1990 .

[12]  Mitchell D. Erickson,et al.  Analytical Chemistry of PCBs , 1986 .