The effect of methylene blue and diaminodiphenysulfone on red cell reduced glutathione synthesis.

Abstract The feeding of diaminodiphenylsulfone (DDS) or injection of methylene blue (MeBI) into rabbits resulted in rapid increase of red cell GSH (reduced glutathione). An increase in the total glutathione level of rabbit red cells was produced in vitro by incubation with DDS or MeBI. These compounds were shown to stimulate the activity of glutathione synthetase both of rabbit and human red cells, but to have little or no effect on γ glutamylcysteine synthetase. It is suggested that the increase of glutathione levels found in humans and in experimental animals after the administration of such drugs is due to stimulation of glutathione synthesis by activation of red cell glutathione synthetase.

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