Introduction to the physics of molecular imaging with radioactive tracers in small animals

Recent advances have greatly enhanced the three‐dimensional (3D) imaging of radioactive tracers in living animals. this article introduces the physics of imaging behind the imaging methods. The article first discusses the selection of the radiation emitted from the tracer and then the process of tomographic reconstruction or how 3D images are made from imaging around the outside of the animal. The technique of single photon emission computed tomography (SPECT) in which the detection of one X‐ray or gamma ray at a time is employed for image formation is then described. Finally, positron emission tomography (PET) which relies on the simultaneous detection of the pair of gamma‐rays formed when the positron annihilates is presented. J. Cell. Biochem. Suppl. 39: 221–230, 2002. © 2002 Wiley‐Liss, Inc.

[1]  B H Hasegawa,et al.  Absolute quantification of regional myocardial uptake of 99mTc-sestamibi with SPECT: experimental validation in a porcine model. , 2001, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[2]  B.H. Hasegawa,et al.  Use of X-ray CT-defined regions of interest for the determination of SPECT recovery coefficients , 1996, 1996 IEEE Nuclear Science Symposium. Conference Record.

[3]  D. Weber,et al.  Ultra-high-resolution imaging of small animals: Implications for preclinical and research studies , 1999, Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology.

[4]  Simon R. Cherry,et al.  Towards in vivo nuclear microscopy: iodine-125 imaging in mice using micro-pinholes , 2002, European Journal of Nuclear Medicine and Molecular Imaging.

[5]  D. Hnatowich,et al.  Observations on the role of nuclear medicine in molecular imaging , 2002, Journal of cellular biochemistry. Supplement.

[6]  H. Atkins,et al.  Pinhole SPECT: an approach to in vivo high resolution SPECT imaging in small laboratory animals. , 1994, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[7]  J. Booij,et al.  Evaluation of high-resolution pinhole SPECT using a small rotating animal. , 2001, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[8]  Gail D. Stevenson,et al.  Gamma-ray imaging using a CdZnTe pixel array and a high-resolution, parallel-hole collimator , 1999 .

[9]  Donald W. Wilson,et al.  Quantitative analysis of acute myocardial infarct in rat hearts with ischemia-reperfusion using a high-resolution stationary SPECT system. , 2002, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[10]  T G Turkington,et al.  Introduction to PET instrumentation. , 2001, Journal of nuclear medicine technology.

[11]  D. V. Davies,et al.  Techniques of Autoradiography. , 1968 .

[12]  S. Cherry,et al.  Performance evaluation of microPET: a high-resolution lutetium oxyorthosilicate PET scanner for animal imaging. , 1999, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[13]  W C Eckelman,et al.  High resolution PET, SPECT and projection imaging in small animals. , 2001, Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society.

[14]  W. Hendee.  Radioactive isotopes in biological research , 1973 .

[15]  James F. Young,et al.  MicroPET: a high resolution PET scanner for imaging small animals , 1996, IEEE Nuclear Science Symposium Conference Record.

[16]  F. J. Beekman,et al.  Design and simulation of U-SPECT, an ultra-high resolution molecular imaging system , 2002, 2002 IEEE Nuclear Science Symposium Conference Record.

[17]  F. Collins,et al.  Principles of Biochemistry , 1937, The Indian Medical Gazette.

[18]  Reynold F. Brown.  Radioactive Isotopes in Biological Research , 1974 .

[19]  C. Melcher.  Scintillation crystals for PET. , 2000, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[20]  Koon-Pong Wong,et al.  An investigation of coded aperture imaging for small animal SPECT , 2000 .

[21]  Eric C. Frey,et al.  Pinhole SPECT of mice using the LumaGEM gamma camera , 2001 .

[22]  Arion F. Chatziioannou,et al.  Molecular imaging of small animals with dedicated PET tomographs , 2001, European Journal of Nuclear Medicine and Molecular Imaging.

[23]  D. Piwnica-Worms,et al.  Molecular imaging in vivo with PET and SPECT. , 2001, Academic radiology.

[24]  A. W. Rogers.  Techniques of autoradiography , 1967 .

[25]  P. Bruyant.  Analytic and iterative reconstruction algorithms in SPECT. , 2002, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[26]  Grafton D. Chase,et al.  Principles of Radioisotope Methodology , 1967 .

[27]  E. Hoffman,et al.  Performance evaluation of A-SPECT: a high resolution desktop pinhole SPECT system for imaging small animals , 2002 .

[28]  A. Celler,et al.  Dynamic SPECT imaging using a single camera rotation (dSPECT) , 1999 .

[29]  M. Phelps,et al.  PET: the merging of biology and imaging into molecular imaging. , 2000, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[30]  Michael A. King,et al.  CHAPTER 22 – Attenuation, Scatter, and Spatial Resolution Compensation in SPECT , 2004 .

[31]  R. N. Goble,et al.  Performance evaluation of the microPET P4: a PET system dedicated to animal imaging. , 2001, Physics in medicine and biology.

[32]  Christopher L. Hansen,et al.  Digital image processing for clinicians, part III: Spect reconstruction , 2002, Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology.

[33]  A. P. Jeavons,et al.  A 3D HIDAC-PET camera with sub-millimetre resolution for imaging small animals , 1998 .

[34]  S. Cherry,et al.  Physics in Nuclear Medicine , 2004 .

[35]  Y. Yonekura,et al.  Ultra-high resolution SPECT system using four pinhole collimators for small animal studies. , 1995, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[36]  R. Weissleder,et al.  A coded aperture for high-resolution nuclear medicine planar imaging with a conventional Anger camera: experimental results , 2001, 2001 IEEE Nuclear Science Symposium Conference Record (Cat. No.01CH37310).

[37]  R. Jaszczak,et al.  Pinhole collimation for ultra-high-resolution, small-field-of-view SPECT. , 1994, Physics in medicine and biology.