Cell Division in the Yeast Saccharomyces cerevisiae Growing at Different Rates

Flow microfluorometry was employed to examine the cell cycle of Saccharomyces cerevisiae growing at different rates governed by different nitrogen sources. In general, the initiation of budding was indistinguishable from the onset of S phase regardless of growth rate. Although with longer generation times S phase showed some increase in length, most of the increase in generation time resulted from increases in Gl.