Long non-coding RNA CASC15 promotes HCC progression via modulation of Six1 targeted by miR-2355-5p

Background: Long-chain non-coding RNA (LncRNA) plays a key role in the biological processes of tumors. LncRNA CASC15 has been shown to be involved in the development of a variety of tumors. The study aimed to elucidate the mechanism of lncRNA CASC15 in the progression of hepatocellular carcinomas (HCC).Methods: qRT-PCR was used to detect the expression levels of CASC15, miR-2355-5p and Six1 mRNA in HCC tissues and cells. Six1 protein expression levels were detected by Western Blot. CCK-8 experiment, colony formation experiment, Edu staining and Transwell experiment analysis were used to analyze the effects of CASC15, miR-2355-5p and Six1 on cell proliferation, cell invasion and migration. The relationship between CASC15, miR-2355-5p and Six1 was analyzed using bioinformatics analysis and Luciferase.Result: CASC15 was raised in HCC tissues and HCC cells. Down-regulation of CASC15 inhibited the growth, migration, invasion and tumor growth of HCC cells. The expression level of miR-2355-5p was reduced in HCC tissues. In addition, miR-2355-5p inhibitor induced the growth, migration and invasion of HCC cells. MiR-2355-5p was predicted to be a downstream target of CASC15. The expression level of miR-2355-5p was negatively correlated with CASC15 in HCC tumor tissues. Six1 was predicted to be a downstream target of miR-30a-5p. In vitro and in vivo results showed that CASC15/miR-2355-5p can regulate Six1.Conclusion: LncCASC15 regulated the proliferation and invasion of Six1 by binding with miR-2355-5p in HCC, suggesting that CASC15 may be a potential target for HCC.

[1]  F. Gao,et al.  Silencing of Long Non-coding RNA GAS5 Suppresses Neuron Cell Apoptosis and Nerve Injury in Ischemic Stroke Through Inhibiting DNMT3B-Dependent MAP4K4 Methylation , 2020, Translational Stroke Research.

[2]  Hua Yu,et al.  Long noncoding RNA MALAT1 enhances the apoptosis of cardiomyocytes through autophagy modulation. , 2020, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[3]  Lili Li,et al.  SP1 induced long non-coding RNA LINC00958 overexpression facilitate cell proliferation, migration and invasion in lung adenocarcinoma via mediating miR-625-5p/CPSF7 axis , 2020, Cancer Cell International.

[4]  E. Pikarsky,et al.  Are we ready for targeted therapy combinations in HCC? , 2020, Gut.

[5]  Ming-liang Yang,et al.  Pathological significance of tRNA-derived small RNAs in neurological disorders , 2019, Neural regeneration research.

[6]  Shuaimei Xu,et al.  Differential expression of lncRNA/miRNA/mRNA and their related functional networks during the osteogenic/odontogenic differentiation of dental pulp stem cells , 2020, Journal of cellular physiology.

[7]  H. Ueno,et al.  Risk factors for nonalcoholic fatty liver disease after gastrectomy for gastric cancer , 2019, Gastric Cancer.

[8]  Chen Huang,et al.  Circular RNA circNHSL1 promotes gastric cancer progression through the miR-1306-3p/SIX1/vimentin axis , 2019, Molecular Cancer.

[9]  L. Tian,et al.  ERRFI1 Inhibits Proliferation and Inflammation of Nucleus Pulposus and is Negatively Regulated by miR-2355-5p in Intervertebral Disc Degeneration. , 2019, Spine.

[10]  Z. Lu,et al.  Noncoding RNAs Serve as Diagnosis and Prognosis Biomarkers for Hepatocellular Carcinoma. , 2019, Clinical chemistry.

[11]  Ying Zhang,et al.  LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway , 2019, Biological Research.

[12]  Yajing Chu,et al.  Six1 regulates leukemia stem cell maintenance in acute myeloid leukemia , 2019, Cancer science.

[13]  Hai-Feng Xu,et al.  Mechanism of exosomal microRNA-224 in development of hepatocellular carcinoma and its diagnostic and prognostic value , 2019, World journal of gastroenterology.

[14]  Ping Huang,et al.  LINC01296 promotes proliferation, migration, and invasion of HCC cells by targeting miR-122-5P and modulating EMT activity , 2019, OncoTargets and therapy.

[15]  Haiyang Xie,et al.  lncRNA DRHC inhibits proliferation and invasion in hepatocellular carcinoma via c‐Myb‐regulated MEK/ERK signaling , 2018, Molecular carcinogenesis.

[16]  A. Ke,et al.  LncRNA SNHG3 induces EMT and sorafenib resistance by modulating the miR‐128/CD151 pathway in hepatocellular carcinoma , 2018, Journal of cellular physiology.

[17]  Qing Luo,et al.  Low-level shear stress promotes migration of liver cancer stem cells via the FAK-ERK1/2 signalling pathway. , 2018, Cancer letters.

[18]  Nian-cai Jing,et al.  LncRNA CASC15 promotes colon cancer cell proliferation and metastasis by regulating the miR‑4310/LGR5/Wnt/β‑catenin signaling pathway. , 2018, Molecular medicine reports.

[19]  Tianyu Liu,et al.  Visfatin increases miR-21 to promote migration in HCC. , 2018, Cellular and molecular biology.

[20]  J. Zheng,et al.  [Role of microRNA and lncRNA in lens development and cataract formation]. , 2018, [Zhonghua yan ke za zhi] Chinese journal of ophthalmology.

[21]  Bin Xia Yang,et al.  LncRNA HULC promotes epithelial and smooth-muscle-like differentiation of adipose-derived stem cells by upregulation of BMP9. , 2018, Die Pharmazie.

[22]  Shou-Ling Wu,et al.  Medical expenditure for liver cancer in urban China: A 10-year multicenter retrospective survey (2002–2011) , 2018, Journal of cancer research and therapeutics.

[23]  Xiaoshun He,et al.  Exosome-related lncRNAs as predictors of HCC patient survival: a prognostic model. , 2018, American journal of translational research.

[24]  G. Tian,et al.  MiR-429 regulates the metastasis and EMT of HCC cells through targeting RAB23. , 2018, Archives of biochemistry and biophysics.

[25]  J-H Tang,et al.  High expression of LncRNA CASC15 is a risk factor for gastric cancer prognosis and promote the proliferation of gastric cancer. , 2017, European review for medical and pharmacological sciences.

[26]  G. Kristiansen,et al.  Inhibition of Six1 affects tumour invasion and the expression of cancer stem cell markers in pancreatic cancer , 2017, BMC Cancer.

[27]  K. Creek,et al.  Six1 promotes colorectal cancer growth and metastasis by stimulating angiogenesis and recruiting tumor-associated macrophages , 2017, Carcinogenesis.

[28]  J. Clements,et al.  MicroRNA Theranostics in Prostate Cancer Precision Medicine. , 2016, Clinical chemistry.

[29]  T. Xia,et al.  Effective Codelivery of lncRNA and pDNA by Pullulan‐Based Nanovectors for Promising Therapy of Hepatocellular Carcinoma , 2016 .

[30]  W. Feng,et al.  Serum MicroRNA-370 as a potential diagnostic and prognostic biomarker for pediatric acute myeloid leukemia. , 2015, International journal of clinical and experimental pathology.

[31]  T. Mok,et al.  Quantification and utility of monosialylated alpha-fetoprotein in the diagnosis of hepatocellular carcinoma with nondiagnostic serum total alpha-fetoprotein. , 2002, Clinical chemistry.

[32]  S. Eissa,et al.  Quantitation of bcl-2 protein in bladder cancer tissue by enzyme immunoassay: comparison with Western blot and immunohistochemistry. , 1998, Clinical chemistry.