New approaches in GMO detection

The steady rate of development and diffusion of genetically modified plants and their increasing diversification of characteristics, genes and genetic control elements poses a challenge in analysis of genetically modified organisms (GMOs). It is expected that in the near future the picture will be even more complex. Traditional approaches, mostly based on the sequential detection of one target at a time, or on a limited multiplexing, allowing only a few targets to be analysed at once, no longer meet the testing requirements. Along with new analytical technologies, new approaches for the detection of GMOs authorized for commercial purposes in various countries have been developed that rely on (1) a smart and accurate strategy for target selection, (2) the use of high-throughput systems or platforms for the detection of multiple targets and (3) algorithms that allow the conversion of analytical results into an indication of the presence of individual GMOs potentially present in an unknown sample. This paper reviews the latest progress made in GMO analysis, taking examples from the most recently developed strategies and tools, and addresses some of the critical aspects related to these approaches.

[1]  Knut G Berdal,et al.  The modular analytical procedure and validation approach and the units of measurement for genetically modified materials in foods and feeds. , 2004, Journal of AOAC International.

[2]  M. Querci,et al.  Innovative Application of Fluorescent Microsphere Based Assay for Multiple GMO Detection , 2008 .

[3]  Yves Bertheau,et al.  Trends in analytical methodology in food safety and quality: monitoring microorganisms and genetically modified organisms , 2007 .

[4]  Myriam Sneyers,et al.  A PCR-microarray method for the screening of genetically modified organisms , 2009 .

[5]  Y. Picó.  Food toxicants analysis : techniques, strategies and developments , 2007 .

[6]  Shuifang Zhu,et al.  Screening genetically modified organisms using multiplex-PCR coupled with oligonucleotide microarray. , 2006, Biosensors & bioelectronics.

[7]  Arne Holst-Jensen,et al.  PCR technology for screening and quantification of genetically modified organisms (GMOs) , 2003, Analytical and bioanalytical chemistry.

[8]  Hans-Ulrich Waiblinger,et al.  Collaborative trial validation studies of real-time PCR-based GMO screening methods for detection of the bar gene and the ctp2-cp4epsps construct. , 2009, Journal of agricultural and food chemistry.

[9]  Marta Hernández,et al.  Interlaboratory transfer of a PCR multiplex method for simultaneous detection of four genetically modified maize lines: Bt11, MON810, T25, and GA21. , 2005, Journal of agricultural and food chemistry.

[10]  Roberto Confalonieri,et al.  Expanding Horizons in the Validation of GMO Analytical Methods: Fuzzy-based Expert Systems , 2008 .

[11]  H. Permingeat,et al.  Detection and quantification of transgenes in grains by multiplex and real-time PCR. , 2002, Journal of agricultural and food chemistry.

[12]  R. Marchelli,et al.  Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. , 2004, Journal of agricultural and food chemistry.

[13]  Marleen M. Voorhuijzen,et al.  Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction , 2008, BMC Genomics.

[14]  Elena Maestri,et al.  Methods for detection of GMOs in food and feed , 2008, Analytical and bioanalytical chemistry.

[15]  H. Broll,et al.  Collaborative Study of a T-nos Real-Time PCR Method for Screening of Genetically Modified Organisms in Food Products , 2007, Journal für Verbraucherschutz und Lebensmittelsicherheit.

[16]  H Schimmel,et al.  Detection and traceability of genetically modified organisms in the food production chain. , 2004, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[17]  Patrick Philipp,et al.  A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients , 2006, Plant Molecular Biology.

[18]  J. Mano,et al.  Real-time PCR array as a universal platform for the detection of genetically modified crops and its application in identifying unapproved genetically modified crops in Japan. , 2009, Journal of agricultural and food chemistry.

[19]  R. Marchelli,et al.  A microarray platform for parallel detection of five transgenic events in foods: a combined polymerase chain reaction-ligation detection reaction-universal array method. , 2005, Journal of agricultural and food chemistry.

[20]  M. Pla,et al.  Multiplex polymerase chain reaction-capillary gel electrophoresis: a promising tool for GMO screening--assay for simultaneous detection of five genetically modified cotton events and species. , 2009, Journal of AOAC International.

[21]  Wei Dong,et al.  GMDD: a database of GMO detection methods , 2008, BMC Bioinformatics.

[22]  José Luis La Paz,et al.  A new PCR‐CGE (size and color) method for simultaneous detection of genetically modified maize events , 2006, Electrophoresis.

[23]  Despina P Kalogianni,et al.  Advances in molecular techniques for the detection and quantification of genetically modified organisms , 2008, Analytical and bioanalytical chemistry.

[24]  R. Twyman,et al.  Trace and traceability—a call for regulatory harmony , 2008, Nature Biotechnology.

[25]  M. Feinberg,et al.  Quantification of the 35S promoter in DNA extracts from genetically modified organisms using real-time polymerase chain reaction and specificity assessment on various genetically modified organisms, part I: operating procedure. , 2005, Journal of AOAC International.

[26]  Yves Bertheau,et al.  Validation of the performance of a GMO multiplex screening assay based on microarray detection , 2008 .

[27]  K. Gruden,et al.  NAIMA: target amplification strategy allowing quantitative on-chip detection of GMOs , 2008, Nucleic acids research.

[28]  Arne Holst-Jensen,et al.  Coherence between legal requirements and approaches for detection of genetically modified organisms (GMOs) and their derived products. , 2006, Journal of agricultural and food chemistry.

[29]  K. Gruden,et al.  Alternative DNA amplification methods to PCR and their application in GMO detection: a review , 2008 .

[30]  Hermann Broll,et al.  Real-Time PCR-Based Ready-to-Use Multi-Target Analytical System for GMO Detection , 2009 .

[31]  Roberto Corradini,et al.  Development of a peptide nucleic acid array platform for the detection of genetically modified organisms in food. , 2005, Journal of agricultural and food chemistry.

[32]  A. Roda,et al.  New trends in bioanalytical tools for the detection of genetically modified organisms: an update , 2008, Analytical and bioanalytical chemistry.

[33]  H. Waiblinger,et al.  Validation and collaborative study of a P35S and T-nos duplex real-time PCR screening method to detect genetically modified organisms in food products , 2008 .

[34]  K. Gruden,et al.  Detection of genetically modified organisms—closing the gaps , 2009, Nature Biotechnology.