Brief report Gene expression profiling of peripheral T-cell lymphoma including (cid:1)(cid:2) T-cell lymphoma

The gene expression profile of peripheral (cid:1)(cid:2) T-cell lymphoma ( (cid:1)(cid:2) TCL) has not been investigated. Using oligonucleotide mi-croarrays, we analyzed total RNA from 7 patients with (cid:1)(cid:2) TCL (4 hepatosplenic, 1 cutaneous, 1 intestinal, and 1 thyroidal) and 27 patients with (cid:3)(cid:4) TCL (11 peripheral TCL-unspecified, 15 angioimmunoblastic TCL, and 1 hepatosplenic). Unsupervised microarray analyses classified all hepatosplenic (cid:1)(cid:2) TCLs into a single cluster, whereas other (cid:1)(cid:2) TCLs were scattered within the (cid:3)(cid:4) TCL distribution. We identified a T-cell receptor signature gene set, which accurately classified (cid:1)(cid:2) TCL and (cid:3)(cid:4) TCL. A classifier based on gene expression under supervised analysis correctly identified (cid:1)(cid:2) TCL. One case of hepatosplenic (cid:3)(cid:4) TCL was placed in the (cid:1)(cid:2) TCL grouping. (cid:1)(cid:2) TCL signature genes included genes encoding killer cell immunoglobulin-like receptors and killer cell lectin-like receptors. Our results indicate that hepatosplenic (cid:1)(cid:2) TCL is a distinct form of peripheral TCL and sug-gest that nonhepatosplenic (cid:1)(cid:2) TCLs are heterogeneous in gene expression. (Blood. 2009;113:1071-1074) P value cutoff of .005. Hierarchical clustering of genes was performed using the Pearson correlation, and hierarchical clustering of cases was obtained using an average linkage algorithm. We chose WebGestalt using Gene Ontology (GO) hierarchies 20,21 for categorization of functional gene groups and the Kyoto Encyclopedia of Genes and Genomes (KEGG) 22,23 pathway for identification of signaling pathways. In both analyses, we performed a separate hypergeometric test with a P value cutoff of .001.

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