Biomimetic sensors for dioxins detection in food samples

Abstract In this work, gold based quartz crystals were modified with synthetic oligopeptides, designed as biomimetic traps using computational approach, to obtain piezoelectric sensors selective to the dioxins. After the optimisation of the methodology using standard solutions, as a practical analytical application, three food matrices (poultry, eggs and milk) spiked at three different dioxin levels were analysed. The cross-reactivity of the system was also quantified spiking the samples with commercial polychlorinated biphenyls (PCBs) and a mixture of PCBs and dioxins. The response of the sensors was not influenced by any of matrices tested either before or after the cleanup steps. The negligible matrix-effect could lead to a simplification of the extraction procedure. Basing on this screening method a rapid assignment of the samples to different degrees of toxicity could be possible reducing the use of high-resolution gas chromatography and high-resolution mass spectrometry (HRGC/HRMS).

[1]  R. Paolesse,et al.  Piezoelectric sensors for dioxins: a biomimetic approach. , 2004, Biosensors & bioelectronics.

[2]  C. Bradfield,et al.  A competitive binding assay for 2,3,7,8-tetrachlorodibenzo-p-dioxin and related ligands of the Ah receptor. , 1988, Molecular pharmacology.

[3]  S. Seidel,et al.  Ah receptor-based chemical screening bioassays: application and limitations for the detection of Ah receptor agonists. , 2000, Toxicological sciences : an official journal of the Society of Toxicology.

[4]  Guomin Shan,et al.  Highly sensitive dioxin immunoassay and its application to soil and biota samples , 2001 .

[5]  Hans-Joachim Böhm,et al.  The computer program LUDI: A new method for the de novo design of enzyme inhibitors , 1992, J. Comput. Aided Mol. Des..

[6]  S. Sakai,et al.  Combinatorial bio/chemical analysis of dioxin and dioxin-like compounds in waste recycling, feed/food, humans/wildlife and the environment. , 2001, Environment international.

[7]  G H Eduljee,et al.  Immunochemical analysis for dioxins--progress and prospects. , 1999, The Science of the total environment.

[8]  J. Giesy,et al.  Species-specific recombinant cell lines as bioassay systems for the detection of 2,3,7,8-tetrachlorodibenzo-p-dioxin-like chemicals. , 1996, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[9]  G. Clark,et al.  Trace contamination with dioxin-like chemicals: evaluation of bioassay-based TEQ determination for hazard assessment and regulatory responses , 2001 .

[10]  S. Safe,et al.  Development of bioassays and approaches for the risk assessment of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related compounds. , 1993, Environmental health perspectives.

[11]  S Sakai,et al.  Bioanalytical screening methods for dioxins and dioxin-like compounds a review of bioassay/biomarker technology. , 2001, Environment international.

[12]  A D'Amico,et al.  Technologies and tools for mimicking olfaction: status of the Rome "Tor Vergata" electronic nose. , 1998, Biosensors & bioelectronics.

[13]  M. Denison,et al.  Development and modification of a recombinant cell bioassay to directly detect halogenated and polycyclic aromatic hydrocarbons in serum. , 2000, Toxicological sciences : an official journal of the Society of Toxicology.

[14]  J. Giesy,et al.  Characterization of the H4IIE rat hepatoma cell bioassay as a tool for assessing toxic potency of planar halogenated hydrocarbons in environmental samples , 1991 .

[15]  A. Tanaka,et al.  A theoretical investigation of the conformation changing of dioxins in the binding site of dioxin receptor model; role of absolute hardness–electronegativity activity diagrams for biological activity , 1999 .