Electrophysiological responses of the mouse retina to 12C ions

Phosphenes ("light flashes") have been reported by most astronauts on space missions and by healthy subjects whose eyes were exposed to ionizing radiation in early experiments in particle accelerators. The conditions of occurrence suggested retinal effects of heavy ions. To develop an in vivo animal model, we irradiated the eyes of anesthetized wild-type mice with repeated bursts of 12C ions delivered under controlled conditions in accelerator. 12C ions evoked electrophysiological retinal mass responses and activated the visual system as indicated by responses recorded from the visual cortex. No retinal immunohistological damage was detected. Mice proved a suitable animal model to study radiation-induced phosphenes in vivo and our findings are consistent with an origin of phosphenes in radiation activating the retina.

[1]  Vittorio Porciatti,et al.  Morphological and Functional Abnormalities in the Inner Retina of the rd/rd Mouse , 2002, The Journal of Neuroscience.

[2]  T. Budinger,et al.  Visual Perception of Accelerated Nitrogen Nuclei interacting with the Human Retina , 1972, Nature.

[3]  H. Wässle,et al.  Immunocytochemical analysis of the mouse retina , 2000, The Journal of comparative neurology.

[4]  A. Vacchi,et al.  Space travel: Dual origins of light flashes seen in space , 2003, Nature.

[5]  R A Hoffman,et al.  Light Flashes Observed by Astronauts on Skylab 4 , 1975, Science.

[6]  N. Peachey,et al.  Pharmacological studies of the mouse cone electroretinogram , 2005, Visual Neuroscience.

[7]  Leif E. Peterson,et al.  Uncertainties in estimates of the risks of late effects from space radiation. , 2004, Advances in space research : the official journal of the Committee on Space Research.

[8]  Steven K Fisher,et al.  Morphological characterization of the retinal degeneration in three strains of mice carrying the rd-3 mutation , 2005, Visual Neuroscience.

[9]  J. Robson,et al.  Rod and cone contributions to the a‐wave of the electroretinogram of the macaque , 2003, The Journal of physiology.

[10]  R. Huesman,et al.  Apollo-Soyuz light-flash observations. , 1977, Life sciences and space research.

[11]  P. J. McNulty,et al.  Single-event effects experienced by astronauts and microelectronic circuits flown in space , 1996 .

[12]  L. Pinsky,et al.  Light Flashes Observed by Astronauts on Apollo 11 through Apollo 17 , 1974, Science.

[13]  P. Mcnulty,et al.  Visual phenomena induced by relativistic carbon ions with and without Cerenkov radiation. , 1978, Science.

[14]  Piergiorgio Picozza,et al.  Phosphenes in low earth orbit: survey responses from 59 astronauts. , 2006, Aviation, space, and environmental medicine.

[15]  Neal S. Peachey,et al.  Electrophysiological analysis of visual function in mutant mice , 2003, Documenta Ophthalmologica.

[16]  M. Lavail,et al.  Retinal degeneration in the nervous mutant mouse. III. Electrophysiological studies of the visual pathway. , 2000, Experimental eye research.

[17]  K. Donner,et al.  Light responses and light adaptation in rat retinal rods at different temperatures , 2005, The Journal of physiology.

[18]  P. Picozza,et al.  Positive visual phenomena in space: A scientific case and a safety issue in space travel , 2006, Vision Research.

[19]  R. Barlow,et al.  Anesthesia can cause sustained hyperglycemia in C57/BL6J mice , 2005, Visual Neuroscience.

[20]  E. Gundelfinger,et al.  Molecular dissection of the photoreceptor ribbon synapse , 2005, The Journal of cell biology.