Comparison of some molecular-genetic techniques for identification of Leishmania circulating in natural foci of zoonotic cutaneous leishmaniasis in the central Asia region.

Different molecular-genetic methods were used to identify a cohort of Leishmania strains from natural foci of zoonotic cutaneous leishmaniasis located in Central Asia, on the former USSR territory. The results obtained using isoenzymes, PCR, restriction fragment length polymorphisms of kDNA and molecular hybridization techniques are discussed in terms of their applicability, discrimination power and feasibility for answering questions related to molecular epidemiological research and for detecting mixed Leishmania infections.

[1]  N. Saravia,et al.  Genotypic polymorphisms in experimental metastatic dermal leishmaniasis. , 1995, Molecular and biochemical parasitology.

[2]  W. Degrave,et al.  The Genus Crithidia : Genotypic Diversity amomg Species , 1994 .

[3]  C. Chapman,et al.  A new species of Leishmania isolated from the great gerbil Rhombomys opimus , 1990, Parasitology.

[4]  Strelkova Mv.  [The isoenzyme identification and pathogenic characteristics of clones of Leishmania major, L. sp. nov. and L. gerbilli]. , 1990 .

[5]  U. Peters,et al.  [The isoenzyme identification and pathogenic characteristics of the Leishmania isolated in natural foci of cutaneous leishmaniasis in the USSR]. , 1990, Meditsinskaia parazitologiia i parazitarnye bolezni.

[6]  H. Momen,et al.  Population heterogeneity among clones of New World Leishmania species , 1990, Parasitology.

[7]  S. L. Le Blancq,et al.  Leishmania in the Old World: 1. The geographical and hostal distribution of L. major zymodemes. , 1986, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[8]  S. L. Le Blancq,et al.  Leishmania in the Old World: 4. The distribution of L. donovani sensu lato zymodemes. , 1986, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[9]  K. Mullis,et al.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. , 1985, Science.

[10]  A. Feinberg,et al.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. , 1983, Analytical biochemistry.

[11]  Alekseev An,et al.  Cloning leishmania at the promastigote stage using the Vonbrun micromanipulator , 1977 .

[12]  A. Alekseev,et al.  [Cloning leishmania at the promastigote stage using the Vonbrun micromanipulator]. , 1977, Parazitologiia.

[13]  H. Harris,et al.  Handbook of enzyme electrophoresis in human genetics , 1976 .