Lab-based x-ray nanoCT imaging

Due to the recent development of transmission X-ray tubes with very small focal spot sizes, laboratory-based CT imaging with sub-micron resolutions is nowadays possible. We recently developed a novel X-ray nanoCT setup featuring a prototype nanofocus X-ray source and a single-photon counting detector. The system is based on mere geometrical magnification and can reach resolutions of 200 nm. To demonstrate the potential of the nanoCT system for biomedical applications we show high resolution nanoCT data of a small piece of human tooth comprising coronal dentin. The reconstructed CT data clearly visualize the dentin tubules within the tooth piece.

[1]  H. Hertz,et al.  High‐resolution compact X‐ray microscopy , 2007, Journal of microscopy.

[2]  Anders Holmberg,et al.  High-resolution computed tomography with a compact soft x-ray microscope. , 2009, Optics express.

[3]  Hongtao Cui,et al.  X-ray computed tomography in Zernike phase contrast mode at 8 keV with 50-nm resolution using Cu rotating anode X-ray source , 2007 .

[4]  D. Weber The distribution of peritubular matrix in human coronal dentin , 1968, Journal of morphology.

[5]  P. Withers,et al.  Comparison and combination of imaging techniques for three dimensional analysis of electrical trees , 2015, IEEE Transactions on Dielectrics and Electrical Insulation.

[6]  S. Zabler,et al.  A laboratory X-ray microscopy setup using a field emission electron source and micro-structured reflection targets , 2014 .

[7]  B. Schmitt,et al.  Performance of single-photon-counting PILATUS detector modules , 2009, Journal of synchrotron radiation.

[8]  O. Bunk,et al.  X-ray imaging with the PILATUS 100k detector. , 2008, Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine.

[9]  E. Anderson,et al.  Soft X-ray microscopy at a spatial resolution better than 15 nm , 2005, Nature.

[10]  A. Baez,et al.  Fresnel Zone Plate for Optical Image Formation Using Extreme Ultraviolet and Soft X Radiation , 1961 .

[11]  Jeff Gelb,et al.  Sub-micron resolution CT for failure analysis and process development , 2008 .

[12]  William H. Richardson,et al.  Bayesian-Based Iterative Method of Image Restoration , 1972 .

[13]  Sub-25-nm laboratory x-ray microscopy using a compound Fresnel zone plate. , 2009, Optics letters.

[14]  G. Zech,et al.  Iterative unfolding with the Richardson-Lucy algorithm , 2012, 1210.5177.

[15]  Roger W. Falcone,et al.  New directions in X-ray microscopy , 2011 .

[16]  L. Lucy An iterative technique for the rectification of observed distributions , 1974 .

[17]  Alexander Sasov,et al.  New Lens‐Free X‐ray Source for Laboratory Nano‐CT with 50‐nm Spatial Resolution , 2011 .

[18]  G. Hounsfield Computerized transverse axial scanning (tomography). 1. Description of system. , 1973, The British journal of radiology.

[19]  R. Hanke,et al.  Realization of a computed tomography setup to achieve resolutions below 1 μm , 2008 .

[20]  M. Firsching,et al.  Development of a Timepix based detector for the NanoXCT project , 2015 .

[21]  Klaus Bergmann,et al.  Compact soft x-ray microscope using a gas-discharge light source. , 2008, Optics letters.

[22]  N. Uhlmann,et al.  Upcoming challenges in high-resolution CT below 1 μm , 2009 .

[23]  A. Mozzanica,et al.  Characterization and Calibration of PILATUS Detectors , 2009, IEEE Transactions on Nuclear Science.

[24]  Chris Jacobsen,et al.  The history and future of X-ray microscopy , 2009 .

[25]  S. Weiner,et al.  Peritubular dentin formation: crystal organization and the macromolecular constituents in human teeth. , 1999, Journal of structural biology.

[26]  Veerle Cnudde,et al.  Recent Micro-CT Scanner Developments at UGCT , 2014 .