The impact of physical based methods screening and their delivery of better quality hits.

The routine application of high-throughput screening (HTS) of compound files has shifted the bottleneck in target-based drug discovery from hit identification to hit qualification because not all hit molecules have the potential to become leads and drug candidates. Biophysical, label-free detection techniques are generic, direct and information-rich, and there is a growing interest in applying these during hit qualification. Some techniques have seen a rapid development toward enhanced sensitivity and throughput and their application to primary screening is within reach.

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