Silicon-based microfilters for whole blood cell separation

This paper reports on the comparison analysis of four main types of silicon-based microfilter for isolation of white blood cells (WBCs) from red blood cells (RBCs) in a given whole blood. The microfilter designs, namely, weir, pillar, crossflow, and membrane, all impose the same cut-off size of 3.5 μm to selectively trap WBCs. Using human whole blood, the microfilters have been characterized and compared for their blood handling capacity, WBCs trapping efficiency and RBCs passing efficiency. Based on the experimental results, the crossflow microfilter is superior and can be integrated with downstream components for on-chip genomic analysis.

[1]  H. Carlson,et al.  Sorting of White Blood Cells in a Lattice , 1997 .

[2]  R. Astumian.  Thermodynamics and kinetics of a Brownian motor. , 1997, Science.

[3]  Mann A. Shoffner,et al.  Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers. , 1998, Analytical biochemistry.

[4]  Y. Tai,et al.  Micromachined membrane particle filters , 1999 .

[5]  J. Voldman,et al.  Holding forces of single-particle dielectrophoretic traps. , 2001, Biophysical journal.

[6]  J. Sturm,et al.  Continuous Particle Separation Through Deterministic Lateral Displacement , 2004, Science.

[7]  Thomas Laurell,et al.  Acoustic control of suspended particles in micro fluidic chips. , 2004, Lab on a chip.

[8]  T. Crowley,et al.  Isolation of plasma from whole blood using planar microfilters for lab-on-a-chip applications. , 2005, Lab on a chip.

[9]  Luke P. Lee,et al.  Mammalian electrophysiology on a microfluidic platform. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Precise profile control of 3D lateral junction traps by 2D mask layout and isotropic etching , 2005 .

[11]  Larry J Kricka,et al.  Micropillar array chip for integrated white blood cell isolation and PCR. , 2005, Biomolecular engineering.

[12]  Levent Yobas,et al.  Buried microfluidic channel for integrated patch-clamping assay , 2006 .

[13]  A. Undar,et al.  A microfluidic device for continuous, real time blood plasma separation. , 2006, Lab on a chip.

[14]  Luke P. Lee,et al.  Single-cell enzyme concentrations, kinetics, and inhibition analysis using high-density hydrodynamic cell isolation arrays. , 2006, Analytical chemistry.

[15]  J. Sturm,et al.  Deterministic hydrodynamics: Taking blood apart , 2006, Proceedings of the National Academy of Sciences.

[16]  David W Inglis,et al.  Critical particle size for fractionation by deterministic lateral displacement. , 2006, Lab on a chip.

[17]  V. Vandelinder,et al.  Separation of plasma from whole human blood in a continuous cross-flow in a molded microfluidic device. , 2006, Analytical chemistry.

[18]  Shoji Takeuchi,et al.  A trap-and-release integrated microfluidic system for dynamic microarray applications , 2007, Proceedings of the National Academy of Sciences.

[19]  Jing Li,et al.  Microfluidic systems for extracting nucleic acids for DNA and RNA analysis , 2007 .