The Separation of Subgroup A and J ALV in Soft Tissue Sarcomas of "817" Broiler Hybrids

The jugular soft tissue sarcomas were obtained from "817" broiler hybrids.The tissue and cell lysis solution was prepared and inoculated in CEF cells for 12 days′ culture,as well as "817" Broiler Hybrids and SPF chickens,ALV-A and ALV-J viruses were identified by IFA from the culture and then to be named as SD1005.The duplicate soft tissue sarcomas of "817" Broiler Hybrids were prepared to culture and touch the piece,and then ALV-A and ALV-J were identified by IFA.In the meantime,ALV-A and ALV-J were discovered co-infection in the same cell.ALV-A and ALV-J were identificated with two pairs of primers toward ALV-A and ALV-J by PCR respectively.The result of autogenous variation analysis of env gene(1 710 bp) to ALV-J indicated that the homology between SD1005 and 14 reference strains at home and abroad was 88.5%-94.0%,the highest homology of which with U.S.A strains 0661 and ADOL-Hc1 were 94.0%,93.8%.The present results indicated that there were mixed viruses in the soft tissue sarcomas from "817" broiler hybrids,with acute and high tumorigenicity.However,whether it was caused by ALV-A and ALV-J and the underlying mechanism of its tumorigenicity needs to be investigated further.